| 3 Counties Car Sales Ltd | Wholesale & retail | 2016 |
| 35 Osier Way Limited | Households | 2025 |
| 3E Capital Partners LLP | | 2017 |
| 8 & 9 Printworks Close Management Company Limited | Real estate | 2017 |
| 90 Entertainment Limited | Information & communication | 2014 |
| A Atherton Consulting Ltd | Professional & scientific | 2017 |
| A C Concierge X Events Limited | Hospitality | 2024 |
| A J Myers Ltd | Professional & scientific | 2013 |
| A Life Inspired Ltd | Health & social care | 2026 |
| A.G.Morgan,Limited | Real estate | 1946 |
| Abbeymill Developments Holdings Limited | Real estate | 2019 |
| Abbeymill Estates Limited | Construction | 2019 |
| Abbeymill Holdings Limited | Financial services | 2018 |
| Abbeymill Homes Limited | Construction | 1991 |
| Abbots Heating and Plumbing Ltd | Construction | 2021 |
| Accelerate Automation Limited | Information & communication | 2023 |
| Ace Flyers Ltd | Other services | 2014 |
| Acsj Limited | Hospitality | 2008 |
| Adept Asset Solutions Ltd | Administrative & support | 2015 |
| Advance Micro Computers Limited | Wholesale & retail | 1984 |
| Advantage Africa | Public administration | 2002 |
| Aeiou Consulting Limited | Information & communication | 1999 |
| Aerospace Investments Co. Ltd | Administrative & support | 2005 |
| AI Lotus Ltd | Professional & scientific | 2026 |
| AJM Management Consulting Ltd | Professional & scientific | 2026 |
| AJT Accountants Limited | Professional & scientific | 2013 |
| ALB Carpentry & Construction Ltd | Construction | 2024 |
| Allens of Olney Limited | Wholesale & retail | 1946 |
| ALM Healthcare Management Ltd | Professional & scientific | 2023 |
| Almax Limited | Professional & scientific | 1991 |
| Amphawan Limited | Hospitality | 2015 |
| Andrew Davidson Consulting Ltd | Health & social care | 2021 |
| Andsco Ltd | Information & communication | 2017 |
| Andy Sharpe Metrology Limited | Professional & scientific | 2023 |
| Andy's Artisan Ventures Ltd | Administrative & support | 2020 |
| Aneris Limited | Professional & scientific | 2009 |
| Anna Gradman Ltd | Information & communication | 2020 |
| Annox Limited | Extraterritorial | 2024 |
| Anorak Boutique Ltd | Wholesale & retail | 2019 |
| Another Vanity Project Ltd | Administrative & support | 2025 |
| Anthony R Property Limited | Real estate | 2025 |
| Antoinette Oglethorpe Limited | Professional & scientific | 2012 |
| Anzen Technologies Limited | Professional & scientific | 2023 |
| Appliance Hire Ltd | Administrative & support | 2024 |
| Ascendia Consulting Partners Ltd | Professional & scientific | 2025 |
| Aspire Technical Services Ltd | Administrative & support | 2018 |
| Auburn Properties Limited | Real estate | 1998 |
| Awakening Films Ltd | Information & communication | 2026 |
| Axis Properties Limited | Real estate | 1985 |
| Axis Three Limited | Construction | 2022 |
| Axis Two Limited | Construction | 2002 |
| Azolla Biotech Limited | Professional & scientific | 2024 |
| B&G Consultants Ltd | Information & communication | 2019 |
| BAD and Boojie Coffee Ltd | Manufacturing | 2023 |
| Baka Tree Collective Ltd | Administrative & support | 2025 |
| Balance Systems Innovations Ltd | Wholesale & retail | 2013 |
| Bannerman Consulting Ltd | Professional & scientific | 2020 |
| Barefoot MS Ltd | Professional & scientific | 2018 |
| Batbox Limited | Manufacturing | 2003 |
| Batchelor Air Conditioning & Refrigeration Limited | Energy | 2001 |
| BDP (Hightown) Ltd | Construction | 2017 |
| BDS Flooring Solutions Ltd | Construction | 2014 |
| Beccy Gillatt School of Jewellery Ltd | Manufacturing | 2022 |
| Becky Wemyss Consulting Limited | Professional & scientific | 2025 |
| Bedford Opto-Technology Limited | Real estate | 1981 |
| Bedford Painting Ltd | Construction | 2013 |
| Beehive HR Limited | Extraterritorial | 2021 |
| Beehive Outsourcing Limited | Professional & scientific | 2020 |
| Belgrade Holdings Limited | Real estate | 1997 |
| Belgrade Investments Limited | Professional & scientific | 2009 |
| Bell and Bear Limited | Hospitality | 2021 |
| BEN & BEN Productions Ltd. | Manufacturing | 2023 |
| Benartie Limited | Wholesale & retail | 2018 |
| Bepg Ltd | Financial services | 2023 |
| Bessemer Fields Residents Management Company Limited | Households | 2022 |
| Better Vape Than Never Ltd | Wholesale & retail | 2023 |
| Beyond the Break Coaching Ltd | Other services | 2025 |
| Bezalel Cornerstone Construction Ltd | Construction | 2026 |
| Bezares UK Limited | Wholesale & retail | 2018 |
| Big Brands Direct LLP | | 2016 |
| Big Brands Storage Limited | Other services | 2020 |
| Bill Hoten Education Training & Consultancy Limited | Education | 2016 |
| Billing Properties NO 2 Limited | Real estate | 2005 |
| Billy Ruffian Limited | Manufacturing | 2018 |
| BK Tyres Limited | Wholesale & retail | 2013 |
| Bloom Outsourcing Limited | Professional & scientific | 2021 |
| Blue Cat Associates Limited | Construction | 2024 |
| Bluebell Strategy Limited | Administrative & support | 2025 |
| Bluerock Secured Finance Limited | Financial services | 2009 |
| Bluestone Technologies Limited | Information & communication | 2017 |
| Bluewave GAS Heating Systems Limited | Construction | 2018 |
| Bodhispace Limited | Health & social care | 2019 |
| Bodylines Specialist Panel Beaters Ltd | Wholesale & retail | 1991 |
| Bond Window Cleaning Limited | Administrative & support | 2024 |
| BOO Gallery Ltd | Wholesale & retail | 2012 |
| Bouche Consultancy Limited | Professional & scientific | 2024 |
| Brands Dispense Limited | Professional & scientific | 2022 |
| Bray Partners Ltd | Professional & scientific | 2023 |
| Brayfield Farms Limited | Agriculture & fishing | 2011 |
| Bridgehurst Property Ltd | Real estate | 2019 |