| John Butler Contracts Limited | Construction | 1995 |
| John Cawley (Properties) Limited | Real estate | 1976 |
| John Clarke Sports Nutrition Limited | Wholesale & retail | 2021 |
| John Courier Ltd | Transport & logistics | 2020 |
| John Darby (1959) Limited | Agriculture & fishing | 1959 |
| John Dory Properties Limited | Real estate | 2007 |
| John E. Reeve Limited | | 1984 |
| John E. Wright & Co. Limited | Manufacturing | 1940 |
| John Exley Ltd | Extraterritorial | 2013 |
| John Fowkes Architects Limited | Professional & scientific | 2011 |
| John Fuchs Business Solutions Limited | Financial services | 2017 |
| John G Peck Limited | Administrative & support | 1999 |
| John G. Hogg Funeral Directors Ltd | Extraterritorial | 2008 |
| John Hallam Carpets Limited | Wholesale & retail | 1984 |
| John Harrison (Lowdham) Limited | Wholesale & retail | 1957 |
| John Head Limited | | 1978 |
| John Hoffman Consultancy Limited | Professional & scientific | 2018 |
| John Hooper & Co. Limited | Professional & scientific | 2009 |
| John Howard Consulting Ltd | Professional & scientific | 2023 |
| John Hughes and Co. (Heating) Limited | | 1950 |
| John Jennings Builders Limited | Construction | 2013 |
| John K Ltd | Professional & scientific | 2010 |
| John Kerr Associates Limited | Administrative & support | 2013 |
| John LEA Limited | Professional & scientific | 2013 |
| John M K Surveying Ltd | Construction | 2025 |
| John M Rowles Limited | Real estate | 2011 |
| John Macnab Services Limited | Manufacturing | 1963 |
| John Mcgovern Limited | Arts & recreation | 2002 |
| John Megálos Ltd | Wholesale & retail | 2025 |
| John Mills Engineering Ltd | Wholesale & retail | 2003 |
| John Morris Architects Ltd | Professional & scientific | 2010 |
| John Nicholls Consultants Ltd | Professional & scientific | 2004 |
| John Osborne Landscape Services Ltd. | Administrative & support | 2000 |
| John Owen Financial Limited | Financial services | 2013 |
| John Paul Furniture Ltd | Manufacturing | 2020 |
| John Pears Limited | Professional & scientific | 2013 |
| John Proctor Limited | Arts & recreation | 2007 |
| John PYE & Sons Holdings Limited | Financial services | 2004 |
| John PYE & Sons Limited | Wholesale & retail | 1990 |
| John PYE Auctions Limited | Extraterritorial | 2022 |
| John PYE Surveyors Limited | Wholesale & retail | 2024 |
| John Rose Jewellers Limited | Wholesale & retail | 2009 |
| John Rourke Creative Limited | Professional & scientific | 2011 |
| John Ryan Group Limited | Construction | 2013 |
| John Servicing Limited | Wholesale & retail | 2018 |
| John Shepherd Electrical Contractors Limited | Construction | 2015 |
| John Sherwin VW & Audi Specialist Limited | Wholesale & retail | 2003 |
| John Shippey Ltd | Professional & scientific | 2008 |
| John Stott Limited | Wholesale & retail | 2013 |
| John Townsend Limited | Administrative & support | 2019 |
| John Vaulkhard (Printing) Limited | Real estate | 1974 |
| John William Kirby Limited | Financial services | 2020 |
| John Winstanley Properties Limited | Construction | 2004 |
| John Woolley Boilersmiths Limited | Other services | 2011 |
| John-Mac and Company International Ltd | | 2008 |
| Johnams Plumbing & Fixing Limited | Manufacturing | 2024 |
| Johnnos Global Ltd | Wholesale & retail | 2024 |
| Johnnuel Limited | Health & social care | 2013 |
| Johnny CHI WHA Limited | Hospitality | 2007 |
| Johnny Fingers Home Appliances Ltd | Professional & scientific | 2022 |
| Johnsmith Kitchens & Interiors Limited | Manufacturing | 2015 |
| Johnson and Lubbock Partners Limited | Professional & scientific | 2019 |
| Johnson and White Ltd | Wholesale & retail | 2017 |
| Johnson Business Properties Limited | Real estate | 2016 |
| Johnson Computer Consulting Limited | Information & communication | 1995 |
| Johnson Enterprise & Consultancy Ltd | Professional & scientific | 2026 |
| Johnson Lets Limited | Real estate | 2020 |
| Johnson Refrigeration Ltd. | Manufacturing | 2018 |
| Johnson&Sonpubsolutions Ltd | Hospitality | 2025 |
| Johnsonholidays Limited | Administrative & support | 2022 |
| Johnsons and Partners Lettings Limited | Real estate | 2023 |
| Johnsons Catering Equipment Ltd | Wholesale & retail | 2002 |
| Johnsons News Retail Ltd | Wholesale & retail | 2022 |
| Johnsons of Keyworth Limited | Hospitality | 2013 |
| Johnsons Vending Services Limited | Administrative & support | 2006 |
| Johnston Accommodation Group Limited | Hospitality | 2026 |
| Johnston Brown Associates Limited | Construction | 1998 |
| Johnston Consultancy Limited | Professional & scientific | 2003 |
| Johnston Plumbing Heating & Joinery Limited | Construction | 2008 |
| Johnston Properties (Nottingham) Limited | Real estate | 2022 |
| Johny Recovery Limited | Transport & logistics | 2025 |
| Joico Ltd | Wholesale & retail | 2022 |
| Joinery by Design (Nottingham) Limited | Manufacturing | 2003 |
| Joint Approach Rehab Ltd | Health & social care | 2021 |
| Joint Effort Fitness Ltd | Health & social care | 2002 |
| Joint Premium Finance Limited | Financial services | 2006 |
| Joint Venture Contracts Ltd | Construction | 2002 |
| Joints & Spine Rehab Ltd | Health & social care | 2026 |
| Joita Ltd | Transport & logistics | 2016 |
| Jojo Auto Limited | Wholesale & retail | 2024 |
| Jojo Divine Services Limited | Administrative & support | 2025 |
| Jojo Garage and Services Limited | Wholesale & retail | 2017 |
| Jojoautogroup Ltd | Wholesale & retail | 2025 |
| JOK Property Ltd | Real estate | 2022 |
| Jokhio Medical Limited | Health & social care | 2021 |
| Jolipe Limited | Construction | 2013 |
| Jolly Collection Ltd | Extraterritorial | 2012 |
| Jolly Griffin Ltd | Information & communication | 2021 |
| Jollytech Solutions Ltd | Information & communication | 2024 |
| Jolo Capital Limited | Financial services | 2019 |