| Houghton Weavers (Management) Ltd. | Arts & recreation | 1984 |
| Houghton Weavers Leisure Limited | Arts & recreation | 2017 |
| House of Bambooo Limited | Real estate | 2025 |
| Houston Advisory Limited | Professional & scientific | 2014 |
| Howell Griffiths Ltd | Professional & scientific | 2022 |
| HRD Strategy Ltd | Professional & scientific | 2021 |
| HRL Partnership Ltd | Transport & logistics | 2025 |
| HRS Cladding Limited | Construction | 2024 |
| HRS Hire Limited | Administrative & support | 2025 |
| HRS Invest Limited | Real estate | 2024 |
| Hudson Consultants Limited | Professional & scientific | 2005 |
| Hudson Consulting Ltd | Professional & scientific | 2020 |
| Hughes Accounting Services Ltd | Professional & scientific | 2017 |
| Hughes Fabrications Limited | Manufacturing | 2016 |
| Hughes Moss Business Solutions Limited | Administrative & support | 2016 |
| Hunter Films Ltd | Professional & scientific | 2025 |
| Hunter Scaffolding Services Ltd | Construction | 2026 |
| Hurrell Homes Limited | Real estate | 2013 |
| Hurst Nuclear Safety Limited | Professional & scientific | 2025 |
| Hush Brewing Co. Ltd | Manufacturing | 2018 |
| Hush TAP Ltd | Hospitality | 2021 |
| Hybrid Venture Surveys Limited | Construction | 2013 |
| Hynes Accounting Limited | Professional & scientific | 2019 |
| Hynes Brothers Limited | Professional & scientific | 2010 |
| Hynes Group Limited | Administrative & support | 2018 |
| Hynescom Ltd | Other services | 2021 |
| Hypersonic Wholesale Ltd | Wholesale & retail | 2021 |
| I G Electrical Commercial Limited | Construction | 2016 |
| I Travels Limited | Administrative & support | 2024 |
| I. G. Ashbrook Fluids Handling Limited | Administrative & support | 2002 |
| I.D. Glass & Glazing Limited | Construction | 2006 |
| Iagent Homes Limited | Real estate | 2015 |
| Iagent Lettings (Northwich) Limited | Real estate | 2025 |
| Iagent Lettings Limited | Real estate | 2015 |
| IAN Howarth Consultancy Services Limited | Administrative & support | 2003 |
| IAN Steele Ltd | Construction | 2023 |
| Ibis Court Management Company Limited | Households | 1991 |
| ICA Sports Ltd | Professional & scientific | 2023 |
| Ice Cream Parlour (Chester) Ltd | Hospitality | 2025 |
| Icode.Net Limited | Information & communication | 2007 |
| Iconic Salons Ltd | Other services | 2011 |
| IDE Furniture and Decoration Ltd | Manufacturing | 2020 |
| Ideascape Ltd | Information & communication | 2003 |
| Iden Industries Ltd | Construction | 2020 |
| Identipay Limited | Information & communication | 2026 |
| IDO Ltd | Wholesale & retail | 2022 |
| IE & TM Speed Limited | Agriculture & fishing | 2008 |
| Ifps Consulting Ltd | Professional & scientific | 2019 |
| Igbineweka Mental Health Care Ltd | Health & social care | 2026 |
| Ignetica Limited | Professional & scientific | 2003 |
| Ignitus Partners Limited | Professional & scientific | 2017 |
| Iguana Holdings Ltd | Real estate | 2023 |
| IJ Bell Limited | Professional & scientific | 2013 |
| IJ Mech Ltd | Professional & scientific | 2024 |
| Illoom Balloon Limited | Wholesale & retail | 2006 |
| Imerys PCC UK Limited | Manufacturing | 1998 |
| Immersion Group UK Ltd | Information & communication | 2019 |
| IMP Property Holdings Ltd | Financial services | 2022 |
| Impact Ellesmere Port CIC | Education | 2011 |
| Impact Justified Limited | Professional & scientific | 2025 |
| Impactus Data Solutions Limited | Real estate | 2011 |
| Impey Improvements Ltd | Construction | 2026 |
| Incamesh Filtration Limited | Manufacturing | 1978 |
| Incline IT Limited | Information & communication | 2019 |
| Indian Northwich Ltd | Hospitality | 2018 |
| Infin-It Limited | Information & communication | 2025 |
| Inforlogic Limited | Information & communication | 2013 |
| Information Catalyst for Enterprise Ltd | Information & communication | 2011 |
| Infosec Zone Ltd | Information & communication | 2013 |
| Infracast Concrete Ltd | Manufacturing | 2026 |
| Infrastructure Management Services Limited | Professional & scientific | 2023 |
| Inhabit Property Management Limited | Real estate | 2020 |
| Initiatives (Design) Limited | Professional & scientific | 2011 |
| INNOV8ACTIVE Limited | Professional & scientific | 2023 |
| Innovatearth Ltd | Wholesale & retail | 2023 |
| Innovo Interim Solutions Limited | Administrative & support | 2024 |
| Inox Group Ltd | Construction | 2024 |
| Insight L&D Ltd | Education | 2024 |
| Insight to Onsite Consulting Ltd | Professional & scientific | 2024 |
| Inspire to Outstand Ltd | Public administration | 2019 |
| Inspired Art & Decorating Ltd. | Construction | 2015 |
| Inspirit Retreat Limited | Arts & recreation | 2016 |
| Instant Logic Limited | Information & communication | 2010 |
| Insync Matchmaking Ltd | Administrative & support | 2017 |
| Integrated MEP Limited | Construction | 2011 |
| Integrity Compliance Solutions Ltd | Information & communication | 2020 |
| Integrity Supplies UK Limited | Wholesale & retail | 2006 |
| Integromd Limited | Professional & scientific | 2017 |
| Intelcoms Limited | Information & communication | 2025 |
| Intelligent Homes of Cheshire Ltd | Construction | 2017 |
| Interchemie Limited | Professional & scientific | 2001 |
| Interchemie Werken "DE Adelaar" BV Limited | Professional & scientific | 2001 |
| Interior Concepts Online Ltd | Wholesale & retail | 2024 |
| International Carbon Alliance Limited | Professional & scientific | 2022 |
| International Fasteners and Fixings Limited | Other services | 2015 |
| International FUN & Team Athletics | Arts & recreation | 2000 |
| Intrinsic Accounting Support Ltd | Professional & scientific | 2022 |
| Intuitive Light Coaching Limited | Education | 2024 |
| Invent Energy Ltd | Professional & scientific | 2022 |
| Inventus IT Ltd | Wholesale & retail | 2017 |