| Raspberry Leaf Interiors Ltd | Wholesale & retail | 2023 |
| RBM Building Services Ltd | Construction | 2013 |
| RBM Building Solutions Ltd | Construction | 2019 |
| Rebel Scot Limited | Manufacturing | 2012 |
| Recruitwork Solutions Limited | Information & communication | 2010 |
| Red Review Ltd | Other services | 2006 |
| Reef Optical Ltd | Professional & scientific | 2024 |
| Reghc Ltd | Professional & scientific | 2026 |
| Reidirect Limited | Information & communication | 2016 |
| Reilly Structures Limited | Professional & scientific | 2025 |
| RG Developments Ltd | Construction | 2018 |
| Rhanna Investments Limited | Financial services | 2024 |
| Rhona Meikle Dog Training Ltd | Agriculture & fishing | 2016 |
| Richard Donnelly Limited | Information & communication | 2019 |
| Robertson Miller Limited | Administrative & support | 2023 |
| Robin Woods Catering Ltd | Hospitality | 2023 |
| Rock & Castle Limited | Hospitality | 2021 |
| Rock White Limited | Professional & scientific | 2021 |
| Rogue Village CIC | Arts & recreation | 2016 |
| Rogue Village Productions Ltd | Arts & recreation | 2019 |
| Rohallion Limited | Information & communication | 2016 |
| Rolhill Limited | Real estate | 2010 |
| ROOM8 Studio Ltd | Information & communication | 2014 |
| Royal Scottish Highland Games Association Limited | Arts & recreation | 1997 |
| Rufaro Limited | Real estate | 2021 |
| Rynord Ltd | Professional & scientific | 1983 |
| Save the Oceans Limited | Professional & scientific | 2025 |
| Saxonfield Partners Limited | Professional & scientific | 2024 |
| SB Quarry (Airdrie) Ltd | Mining & quarrying | 2012 |
| Scott Properties (Edinburgh) Limited | Construction | 1980 |
| Scottish Seabird Centre | Arts & recreation | 1997 |
| Scottish Seabird Centre Trading Limited | Wholesale & retail | 1998 |
| Seacliff Events Ltd | Arts & recreation | 2021 |
| Seacliff Investment Company | Professional & scientific | 2017 |
| Seonamara Properties Ltd | Real estate | 2026 |
| Shields Site Services Ltd | Construction | 2025 |
| SI Brands Limited | Wholesale & retail | 2023 |
| Simon Bailey Consulting Ltd | Professional & scientific | 2023 |
| Size Nine ELM Limited | Information & communication | 2024 |
| Skye High Ltd | Hospitality | 2021 |
| Solja Limited | Professional & scientific | 2012 |
| Somner Macdonald Lothian Limited | Professional & scientific | 2009 |
| Sonamara Limited | Information & communication | 2021 |
| Southern Transitions Platform Limited | Professional & scientific | 2024 |
| Space Cadet Developments Ltd | Construction | 2024 |
| Sporting Memories Network (Scotland) C.I.C. | Health & social care | 2011 |
| Sprayfix Limited | Construction | 2006 |
| Sprinkler Installations (UK) Limited | Construction | 2002 |
| SRS Scot Ltd | Real estate | 2020 |
| Steampunk Coffee Limited | Hospitality | 2019 |
| Stonehillsalt PR Ltd | Professional & scientific | 2015 |
| Stora Scotland Limited | Professional & scientific | 2023 |
| Superfry Potatoes Ltd. | Wholesale & retail | 1959 |
| Susie Lang Management Ltd. | Professional & scientific | 2003 |
| Sutherland & Co Architects Limited | Professional & scientific | 2012 |
| Swanned Ltd | Information & communication | 2024 |
| Swell Investing Ltd | Financial services | 2026 |
| Syncai Ltd | Information & communication | 2025 |
| Tantallon Projects Ltd | Construction | 2026 |
| Tantallon Seismic Limited | Professional & scientific | 2011 |
| Tartan Commercial Limited | Real estate | 2016 |
| Tepee Investments (UK) Limited | Financial services | 2024 |
| Terraforma MX Circuit Design Limited | Administrative & support | 2009 |
| Terraforma Plant Hire Ltd | Administrative & support | 2017 |
| That Rosie Glow North Berwick Ltd | Other services | 2020 |
| THE Buckled Bull Bars Limited | Hospitality | 2025 |
| THE Crafty KIT Company Ltd | Arts & recreation | 2013 |
| THE Edge Coaching and Consulting Ltd | Professional & scientific | 2018 |
| THE Glen Private Hire Ltd | Other services | 2020 |
| THE Global Concerns Trust | Education | 1996 |
| THE Hand & Chisel Limited | Manufacturing | 2025 |
| THE Hunter Physiotherapy and Sports Injury Clinic Limited | Other services | 2005 |
| THE Leprosy Mission Scotland | Health & social care | 2009 |
| THE Little Phonics Company Ltd | Education | 2026 |
| THE Makers LAB Ltd | Information & communication | 2017 |
| THE Modern Mainframe Ltd | Information & communication | 2024 |
| THE North Berwick Flower Company Ltd | Agriculture & fishing | 2024 |
| THE North Berwick Trust Ltd | Administrative & support | 2018 |
| THE Physiotherapy Place Limited | Health & social care | 2016 |
| THE Renaissance Club at Archerfield, LLP | | 2005 |
| THE South African Butcher Ltd | Wholesale & retail | 2026 |
| THE Traditional Golf Company Ltd | Arts & recreation | 2025 |
| THE Whins Game Farm Ltd | Agriculture & fishing | 2021 |
| THE Wise Folly Ltd | Hospitality | 2018 |
| Thirlestane Properties Ltd | Real estate | 2026 |
| Thomson Dental Care Limited | Health & social care | 2021 |
| Tobermory Oysters Limited | Agriculture & fishing | 2014 |
| TRG Hair Limited | Other services | 2023 |
| Tutto Bene Brands Ltd | Administrative & support | 2018 |
| Tyninghame Gardens Limited | Households | 1989 |
| UK Property Professionals Limited | Information & communication | 2010 |
| UP NB Limited | Professional & scientific | 2013 |
| Upper Class Travel Limited | Administrative & support | 2013 |
| Vagabond Documentaries Limited | Arts & recreation | 2024 |
| Varss Limited | Education | 2022 |
| Volt 4X4 Ltd | Manufacturing | 2024 |
| Vorin Build Studio Ltd | Manufacturing | 2026 |
| W'Est Solutions (Edinburgh) Limited | Other services | 2014 |
| Wamphray Ltd | Agriculture & fishing | 2012 |
| Ware Marine Limited | Financial services | 2000 |