| Enlightened Holdings Limited | Financial services | 2026 |
| Ennismore Residential Limited | Construction | 2013 |
| Enoteca Worldwide Limited | Wholesale & retail | 2025 |
| Enoz Construction | | 1995 |
| Enriching Minds Group Ltd | Health & social care | 2025 |
| Ensemble Rugby Limited | Arts & recreation | 2016 |
| Entas Limited | Wholesale & retail | 2019 |
| Enterprise Buy and LET Limited | Real estate | 2020 |
| Enterprise D&G Ltd | Construction | 2022 |
| Entertainments NPT Ltd | Financial services | 2022 |
| Entryextra Limited | Hospitality | 2002 |
| Environmental Waste Services Limited | Water & waste | 2017 |
| Envirosavers Limited | Construction | 2003 |
| Envolve Technology Limited | Information & communication | 2013 |
| Eoin Fullerton Podiatry Ltd | Health & social care | 2021 |
| EP Planning Ltd | Professional & scientific | 2026 |
| Epac-Hughes Limited | Professional & scientific | 2016 |
| Epc-Tec Limited | Professional & scientific | 2013 |
| Epco Electronics Limited | Transport & logistics | 1973 |
| Epec Engineered Technologies Limited | Manufacturing | 2022 |
| EPG Properties Limited | Real estate | 2025 |
| Ephesus Pizzeria Ltd | Hospitality | 2023 |
| Epic Insulation Limited | Construction | 2011 |
| Epigen Business Group Ltd | Professional & scientific | 2019 |
| EPP Consultancy Ltd | Financial services | 2021 |
| EPS Commissioning Limited | Construction | 2005 |
| Equalizer Productions Ltd | Administrative & support | 2022 |
| Equianalytix Limited | Information & communication | 2023 |
| Equilibrium Project Limited | Health & social care | 2013 |
| Equinvest Limited | Real estate | 2024 |
| Equipment Parts Solutions Limited | Wholesale & retail | 2021 |
| Ercall Holdings Limited | Financial services | 2024 |
| Erflower Psychotherapy Ltd | Health & social care | 2024 |
| Ergability Limited | Health & social care | 2015 |
| Ergo Investments Ltd | Real estate | 2017 |
| Ergonautic Boats Limited | Manufacturing | 2020 |
| Eriyen Properties Ltd | Real estate | 2023 |
| Ermc Limited | Real estate | 2002 |
| Erwlon C & C Park Limited | Hospitality | 2008 |
| ES Lounge Ltd | Hospitality | 2019 |
| ES20 Consulting Ltd | Professional & scientific | 2026 |
| ES28 Ltd | Real estate | 2013 |
| Escape Festivals Ltd | Administrative & support | 2024 |
| Escape Fire Services Ltd | Public administration | 2015 |
| Escapism Unlocked CIC | Arts & recreation | 2025 |
| Escudero Auto Ltd | Manufacturing | 2021 |
| ESF Media Ltd | Arts & recreation | 2021 |
| Esid Limited | Administrative & support | 2013 |
| ESP South Wales Limited | Extraterritorial | 2025 |
| Espo Transport Ltd | Transport & logistics | 2017 |
| Esquare (Bristol) Limited | Professional & scientific | 1990 |
| Esquare (Holdings) Ltd | Financial services | 2026 |
| Esquare (Properties) Ltd | Real estate | 2025 |
| Esquare Limited | Professional & scientific | 2004 |
| Esquare Management Ltd | Professional & scientific | 2024 |
| Esquare Services Ltd | Professional & scientific | 2025 |
| Esquared Commissioning Services Ltd | Construction | 2025 |
| Esrms Ltd | Professional & scientific | 2018 |
| Ess-Express Courier Limited | Transport & logistics | 2017 |
| Essential 4 Care Ltd | Health & social care | 2021 |
| Essereale Ltd | Manufacturing | 2024 |
| Estavros Trading Limited | Administrative & support | 2010 |
| Esto Goods Ltd | Transport & logistics | 2025 |
| Estrada Holdings Ltd | Real estate | 2024 |
| Estrada Lettings & Management Ltd | Real estate | 2024 |
| Eternal Anugraha Ltd | Wholesale & retail | 2022 |
| Eternal Faith Group Ltd | Health & social care | 2024 |
| Ethical Digital Limited | Manufacturing | 2016 |
| Ethos Financial Management Ltd | Financial services | 2019 |
| Etlp Limited | Professional & scientific | 2014 |
| ETO Contracting Limited | Construction | 2015 |
| EU Logistics Ltd | Professional & scientific | 2019 |
| Eunibeam Healthcare Ltd | Health & social care | 2026 |
| Eureka Business Savings Limited | Administrative & support | 2023 |
| Eureka Capital Allowances Limited | Professional & scientific | 2021 |
| Euro PROPERTIES(2025) Limited | Real estate | 2025 |
| Euro WB Ltd | Manufacturing | 2018 |
| Euro Wonder Warehouse Ltd | Wholesale & retail | 2025 |
| Eurogate Projects Limited | Professional & scientific | 1992 |
| Eurograph Computer Supplies Ltd | Wholesale & retail | 2007 |
| Europe Home Security Installations Ltd | Information & communication | 2009 |
| Europe Home Security Limited | Administrative & support | 2006 |
| European Cathedrals Exhibition Trust | Arts & recreation | 1991 |
| European Devices & IVD Associates Ltd | Health & social care | 2016 |
| European Recruitment Services Ltd | Administrative & support | 2024 |
| Eurosec Limited | Construction | 2004 |
| Eurosec Properties Limited | Real estate | 2025 |
| Euse Resources Ltd | Administrative & support | 2019 |
| Eutivo Ltd | Information & communication | 2026 |
| EV Mobiliti Limited | Professional & scientific | 2015 |
| Ev-Entz.Co.Uk Limited | Wholesale & retail | 2008 |
| EVA Tech Auto Ltd | Wholesale & retail | 2022 |
| Evaframe Ltd | Manufacturing | 2013 |
| Evan Gregg Sales Ltd | Wholesale & retail | 2025 |
| Evan Haezer Limited | Extraterritorial | 2011 |
| Evans Partners (Cardiff) Ltd | Hospitality | 2024 |
| Evans Partners Holdings Ltd | Financial services | 2025 |
| Evans Partners HS Ltd | Hospitality | 2025 |
| Evans Partners JFS Ltd | Hospitality | 2025 |
| Evans Partners Ltd | Hospitality | 2019 |