| Dream Play Ltd | Hospitality | 2026 |
| Drivekey Ltd | Information & communication | 2025 |
| DS Construction and Services Ltd | Construction | 2022 |
| Dstny Communication Solutions UK Ltd | Information & communication | 2022 |
| Dstny Global Services UK Ltd | Information & communication | 2013 |
| Dudu Consultants Ltd | Professional & scientific | 2018 |
| Dunford Roofing Solutions Ltd | Construction | 2025 |
| Dwellix Tech Services Ltd | Information & communication | 2026 |
| DX2 Design Ltd | Information & communication | 2012 |
| Dyalog 2002 Limited | Professional & scientific | 2003 |
| Dyalog APL Limited | Professional & scientific | 2003 |
| Dyalog Limited | Information & communication | 2004 |
| Dyalog SIP Trustees Limited | Professional & scientific | 2014 |
| Dzmml Ltd | Wholesale & retail | 2025 |
| D’Oro Newport Pagnell Ltd | Hospitality | 2024 |
| E a Thorn Ltd | Construction | 2017 |
| E&K Muratis Pizza Ltd | Hospitality | 2017 |
| E.C.R. Service Limited | Manufacturing | 1984 |
| E.J.K. Building Contractors Limited | Construction | 1993 |
| Eakley Stables Air BNB Ltd | Hospitality | 2021 |
| EC7 Limited | Real estate | 2016 |
| ED Gasworx Ltd | Construction | 2020 |
| Edgenlp Limited | Administrative & support | 2009 |
| Edwards Property Investing Ltd | Real estate | 2021 |
| Eezy Rental Ltd | Administrative & support | 2018 |
| Ehra Talent Solutions Limited | Information & communication | 2013 |
| Ekklesia Solution Limited | Information & communication | 2020 |
| Elearningate Ltd | Information & communication | 2000 |
| Elevate IT Services Ltd | Information & communication | 2021 |
| Elle & FEE Ltd | Hospitality | 2022 |
| Ellievisionav Limited | Administrative & support | 2018 |
| Elliot House Loughton Management Company Limited | Real estate | 2005 |
| Elliott Electrical Supplies Limited | Wholesale & retail | 2004 |
| Ellis Mediation Limited | Professional & scientific | 2021 |
| Ellis Samuel Associates Limited | Administrative & support | 2020 |
| Elmac Electrical Limited | Administrative & support | 2009 |
| Eltk Properties Limited | Real estate | 2024 |
| Empowered Business and Training Consulting Limited | Professional & scientific | 2012 |
| Empowered Business Services Ltd | Education | 2025 |
| EN Route Sameday Transport Limited | Administrative & support | 2003 |
| En-Projects Ltd | Professional & scientific | 2007 |
| Energies UK Ltd | Construction | 2021 |
| Engaged Ltd | Information & communication | 2015 |
| Enhance Global Solutions Ltd | Information & communication | 2020 |
| Enid's Services Limited | Health & social care | 2019 |
| Entexcellence LLP | | 2025 |
| Envibra Limited | Extraterritorial | 2011 |
| Equitynest Property Ltd | Real estate | 2026 |
| ES Hanson Ltd | Professional & scientific | 2024 |
| ESK Decorating Contractors Limited | Construction | 2006 |
| Essence Oasis Ltd | Education | 2017 |
| ETA Security Limited | Construction | 2010 |
| Ethos Clinic Limited | Health & social care | 2023 |
| Euroshop Development Limited | Real estate | 2025 |
| Evans Electrical (MK) Ltd | Construction | 2022 |
| Evatron Limited | Extraterritorial | 2005 |
| EVC Investments Ltd | Real estate | 2017 |
| Event & Media Structures Ltd | Construction | 2019 |
| Evergreens (Beaufort Park) Management Company Limited | Households | 2022 |
| Evolveesm Ltd | Information & communication | 2025 |
| Eway Trading Limited | Real estate | 2017 |
| Expert Witness Services Limited | Administrative & support | 2021 |
| Eyo's Laser Clinic Ltd | Other services | 2025 |
| F&P Property Northampton Limited | Real estate | 2021 |
| Facade Information Management Sytems Limited | Information & communication | 2023 |
| Face Haus Limited | Other services | 2022 |
| Factor NOW Ltd | Information & communication | 2026 |
| Fairview Care MK Limited | Health & social care | 2024 |
| Falcon Bond International Ltd | Professional & scientific | 2015 |
| Fallon Alternatives Limited | Professional & scientific | 2022 |
| Family Law Support Ltd | Administrative & support | 2025 |
| Fatz N Skillz Limited | Professional & scientific | 2015 |
| FC Barrister Limited | Professional & scientific | 2022 |
| FC Unity Ltd | Arts & recreation | 2024 |
| Feast Land Ltd | Hospitality | 2026 |
| Featherstone Legal Consulting Limited | Professional & scientific | 2026 |
| Femix Care Services Limited | Health & social care | 2025 |
| Fenco-Aldridge (Barton) Limited | Manufacturing | 1968 |
| Fenland Auto Sales Team Limited | Wholesale & retail | 2019 |
| Fido and Felix Limited | Wholesale & retail | 2010 |
| FIFTY2 Consultancy Limited | Professional & scientific | 2017 |
| Findyourbrand Ltd | Wholesale & retail | 2024 |
| Fine Property Investments Ltd | Real estate | 2019 |
| Firesand Fire and Security Ltd | Administrative & support | 2025 |
| Firesand Limited | Information & communication | 2016 |
| Firesand Strategic Partnerships Limited | Information & communication | 2024 |
| First Choice Wholesaler Ltd | Wholesale & retail | 2024 |
| First Circle Packaging Limited | Manufacturing | 1945 |
| First County Finance (UK) Limited | Financial services | 1990 |
| Fitmk Ltd | Health & social care | 2010 |
| Flagel Compliance Services Limited | Professional & scientific | 2023 |
| Flanagan Consultants Ltd | Information & communication | 2025 |
| Floom Digital Limited | Information & communication | 2025 |
| Flying Object Ltd | Professional & scientific | 2013 |
| FM96 Services Ltd | Information & communication | 2018 |
| FMO Global Limited | Professional & scientific | 2015 |
| Fobdef Care Limited | Health & social care | 2023 |
| Food Technical Solutions Limited | Professional & scientific | 2014 |
| Fools on Horses Limited | Arts & recreation | 2009 |
| Forever Green Homes Limited | Construction | 2016 |