| Neuk Coffee Company Ltd | Wholesale & retail | 2025 |
| Neurosurgery Services Ltd | Health & social care | 2017 |
| Parkhill Construction Services Limited | Construction | 2010 |
| PC LAB Ltd | Wholesale & retail | 2019 |
| Peacefit Limited | Other services | 2018 |
| Peacehill Farming Limited | Agriculture & fishing | 1993 |
| Peterselectricalservices Limited | Construction | 2022 |
| Podiatry Scotland Ltd | Health & social care | 2016 |
| Prospect Terrace Ltd | Professional & scientific | 2018 |
| Pushpin Play Ltd | Information & communication | 2026 |
| Robertson Collaborate Limited | Professional & scientific | 2013 |
| Rose Wood Events & Construction Ltd | Construction | 2021 |
| Ryan Poole Transport Limited | Transport & logistics | 2018 |
| Ryden Rope Access Limited | Construction | 2023 |
| Scooply St.Andrews Ltd | Hospitality | 2025 |
| Scooply TAY Bridgehead Ltd | Manufacturing | 2024 |
| SKP Sound Design Ltd | Information & communication | 2018 |
| Social Juice CIC | Agriculture & fishing | 2022 |
| St.Andrews Private GP Clinic Ltd | Public administration | 2023 |
| Stanford Motors (Wormit) Limited | Manufacturing | 1982 |
| Station Works Properties Ltd. | Real estate | 1983 |
| TAY Bridge Taverns Limited | Hospitality | 2011 |
| TAY Collective Ltd | Information & communication | 2026 |
| TAY River Taxis Limited | Transport & logistics | 2024 |
| TAY Tree Surgery Ltd | Administrative & support | 2020 |
| Taymara | Transport & logistics | 2007 |
| Tayside Real Estate Limited | Real estate | 2004 |
| Tayside Tradesmen Ltd | Construction | 2024 |
| Tending Gardens Limited | Professional & scientific | 2025 |
| THE Electrical Company Services Limited | Construction | 2021 |
| THE Fifie (Newport on TAY) Limited | Hospitality | 2003 |
| THE Grekin Clinic Ltd | Health & social care | 2009 |
| THE Gynae-Oncology Clinic Ltd | Health & social care | 2023 |
| THE Natural Energy Company (Orkney) Limited | Extraterritorial | 2009 |
| THE Natural Energy Company (Scotland) Limited | Construction | 2009 |
| THE Newport Inn Limited | Hospitality | 2025 |
| THE Reformer Studio Newport Ltd | Arts & recreation | 2025 |
| THE Social Edit Ltd | Professional & scientific | 2019 |
| Thepropertypirate Ltd | Extraterritorial | 2025 |
| Trifecta Lifestyle Services Ltd | Other services | 2025 |
| V Office Spaces Ltd | Financial services | 2022 |
| VE Smart Systems Ltd | Professional & scientific | 2022 |
| Vital Hike Limited | Information & communication | 2010 |
| Wbcs (ST Andrews) Ltd | Wholesale & retail | 2011 |
| Wildspire Ltd | Financial services | 2025 |
| Witty Wombat Ltd | Professional & scientific | 2022 |
| Wormiteers Limited | Financial services | 2024 |