| A Todd & Co Limited | Construction | 2015 |
| A&S Holdings 1 (Ayrshire) Ltd | Hospitality | 2024 |
| A&S Holdings 2 (Ayrshire) Ltd | Hospitality | 2024 |
| AA Newmilns Limited | Wholesale & retail | 2018 |
| Adrian Casey Electrical Services Ltd | Construction | 2015 |
| AIS Roofing & Roughcasting Ltd | Construction | 2024 |
| AKO Tyres Ltd | Wholesale & retail | 2026 |
| Amor Holiday Lodge Ltd | Real estate | 2021 |
| AMT Bodyshop Ltd | Construction | 2026 |
| Andeye Ltd | Information & communication | 2020 |
| Aqueum Ltd | Information & communication | 2017 |
| Arda Fish & Chips Ltd | Hospitality | 2017 |
| Arkelstone Ltd | Information & communication | 2026 |
| Asgard Dental Laboratory Ltd | Health & social care | 2016 |
| Authentic Spice Ltd | Hospitality | 2019 |
| Ayrshire Gardening Services Ltd | Administrative & support | 2026 |
| Ayrshire Timber Merchants Limited | Wholesale & retail | 2026 |
| B Devere Limited | Construction | 2025 |
| Burns Trading 2025 Ltd | Hospitality | 2025 |
| BURNS2024 Ltd | Hospitality | 2024 |
| Carrie on Coaching and Advisory Ltd | Health & social care | 2025 |
| CHIPPY2024 Limited | Hospitality | 2024 |
| Commando Factory Gym Ltd | Arts & recreation | 2025 |
| Cros Country Projects Ltd | Construction | 2020 |
| D & T Commercial Ltd | Real estate | 2026 |
| D H Moses Limited | Real estate | 2023 |
| D Harvie Builders Ltd | Construction | 2025 |
| Dalhousie & Richmond Ltd | Real estate | 2025 |
| Dustacco Group Limited | Financial services | 2024 |
| EA Smiles (Scotland) Ltd | Professional & scientific | 2014 |
| Eagle Private Hire Limited | Transport & logistics | 2001 |
| Elite Spray Coatings Ltd | Construction | 2025 |
| Elysium Dynamics Ltd | Information & communication | 2023 |
| Fleetpoint (Ayrshire) Ltd | Professional & scientific | 2025 |
| Fleetpoint Limited | Manufacturing | 2016 |
| Franklin Clark Media Limited | Manufacturing | 2017 |
| G Mcnicol Ltd | Information & communication | 2019 |
| Gills Newmilns Ltd | Wholesale & retail | 2018 |
| GJM Printing Ltd | Manufacturing | 2016 |
| Granite Paving Specialist Limited | Construction | 2008 |
| GVG Forestry Limited | Agriculture & fishing | 2011 |
| Honeybea Farm Therapies CIC | Agriculture & fishing | 2022 |
| Ivade Limited | Education | 2025 |
| J & S Manufacturing Limited | Manufacturing | 2010 |
| Jake Wilson Electrical Ltd. | Construction | 2013 |
| KK Pet Services Ltd | Other services | 2026 |
| Laura Florals Ltd | Agriculture & fishing | 2020 |
| Little Prints Scotland Ltd | Arts & recreation | 2024 |
| Loudoun Mains Country Club CIC | Arts & recreation | 1995 |
| Loudoun Mains Luxury Lodge 2 Limited | Hospitality | 2024 |
| Loudoun Mains Management Limited | Real estate | 1998 |
| Maintainit Scotland Limited | Other services | 2011 |
| Mair Matheson Holdings Ltd | Financial services | 2022 |
| Mair Matheson Solicitors Ltd | Professional & scientific | 2010 |
| Mansaro Limited | Information & communication | 2026 |
| Mary Mcclung Coaching Limited | Health & social care | 2019 |
| Mason Quinn Group Ltd | Construction | 2024 |
| Mckellars of Ayrshire Limited | Agriculture & fishing | 2021 |
| Moran Eyecare Limited | Wholesale & retail | 2008 |
| MOT Point Limited | Wholesale & retail | 2014 |
| Muirhead Farm (Scotland) Ltd | Agriculture & fishing | 2015 |
| Nelson Renovations Ltd | Construction | 2026 |
| New Chippy Ltd | Hospitality | 2024 |
| Newmilns Barber Ltd | Other services | 2025 |
| Newmilns Enterprise Company Limited | Administrative & support | 2015 |
| Newmilns Snow and Sports Complex | Arts & recreation | 2003 |
| Oliveandoscar Ltd | Wholesale & retail | 2023 |
| PN2024 Ltd | Hospitality | 2024 |
| Prime Performance Fitness and Nutrition Ltd | Arts & recreation | 2023 |
| Red Sable Consulting Limited | Professional & scientific | 2026 |
| RGM Access Ltd. | Construction | 2013 |
| Richardton Farm | | 1980 |
| Rizaf Limited | Wholesale & retail | 2025 |
| Sabai Coffee House Ltd | Hospitality | 2025 |
| Saxen Ltd | Wholesale & retail | 2005 |
| SCM Services Ltd | Administrative & support | 2024 |
| Sejm Trading Limited | Hospitality | 2025 |
| Setup Locations Ltd | Administrative & support | 2020 |
| Shark Shop Limited | Manufacturing | 2024 |
| Shawhill Farm Foods Limited | Agriculture & fishing | 2015 |
| Shawhill Organic Farm Ltd | Agriculture & fishing | 2026 |
| Smith Contracts Scotland Limited | Construction | 2014 |
| SP Chippy Ltd | Hospitality | 2025 |
| Stage Flameproofing Limited | Extraterritorial | 2013 |
| Sylvia Beauty Ltd | Other services | 2025 |
| THE Humble Table Ltd | Hospitality | 2023 |
| THE Pilatees Studio Ltd | Arts & recreation | 2026 |
| Today OR Tomorrow Limited | Administrative & support | 2024 |
| True Blue Radio Ltd | Arts & recreation | 2025 |
| TT Events Catering Ltd | Hospitality | 2019 |
| V5 Auto Services Ltd | Wholesale & retail | 2025 |
| Vesuvius UK (2002) Limited | Manufacturing | 1905 |
| Watermiser Newmilns Limited | Extraterritorial | 2025 |
| West Siphonics Limited | Construction | 2004 |
| West Siphonics Systems Limited | Construction | 2010 |
| Winnocklands Ltd | Hospitality | 2022 |