| DLL Flooring Ltd | Wholesale & retail | 2010 |
| DM Carpentry (ELY) Limited | Manufacturing | 2022 |
| DMG Capital Ltd | Financial services | 2018 |
| Donaldson Perring Limited | Construction | 2020 |
| DOT UU Limited | Wholesale & retail | 2022 |
| Downing Pints Ltd | Hospitality | 2024 |
| DP Barber Ltd | Other services | 2024 |
| DPM Bathroom Services Limited | Construction | 2020 |
| DR Tijssen Coaching and Consultancy Ltd | Administrative & support | 2018 |
| Drago Haulage Ltd | Transport & logistics | 2025 |
| Drayson Property Holdings Limited | Real estate | 1973 |
| DS Mortgages Limited | Professional & scientific | 2023 |
| Dsjb Electrical Limited | Construction | 2015 |
| DSL Marketing Limited | Other services | 1994 |
| DT Estate Agents Ltd | Real estate | 2021 |
| DT Holdings ( NKT ) Ltd | Financial services | 2022 |
| DT Ltd | Extraterritorial | 2005 |
| Duane Plant Training Ltd | Education | 2011 |
| Dullingham Estate Farms Limited | Agriculture & fishing | 1960 |
| Dunelm Accountants Limited | Professional & scientific | 2015 |
| Dunlop & Holden Ltd | Real estate | 2017 |
| Durcan Bloodstock Limited | Professional & scientific | 2021 |
| Duverse Holdings Limited | Administrative & support | 2022 |
| DWA Advisory and Consulting Ltd | Agriculture & fishing | 2016 |
| Dylan Cunha Racing Ltd | Arts & recreation | 2021 |
| Dynagroup Ltd | Extraterritorial | 2018 |
| Dysaplyn Limited | Wholesale & retail | 2026 |
| E B S Barbering Limited | Other services | 2024 |
| E C Barbering Limited | Other services | 2022 |
| E J Stocker Construction Ltd | Construction | 2013 |
| E3 Consultancy Ltd | Professional & scientific | 2018 |
| EAG Consultancy Limited | Professional & scientific | 2025 |
| Eagle and Hind Ltd | Hospitality | 2013 |
| Eagle Lane Farm Limited | Agriculture & fishing | 1986 |
| Eara Holdings Ltd | Real estate | 2023 |
| Earthikes Ltd | Information & communication | 2016 |
| East Anglia Bloodstock International LP | | 2012 |
| East Anglia Farm Holdings Ltd | Real estate | 2023 |
| Easy Solutions Services Ltd | Transport & logistics | 2021 |
| EAT Like Home Ltd | Hospitality | 2023 |
| Ebonos Limited | Professional & scientific | 2012 |
| Eclipse Analytics Limited | Professional & scientific | 2020 |
| Eclipse Veterinary Software Limited | Information & communication | 2010 |
| Ecocartonline Ltd | Wholesale & retail | 2025 |
| Ecsec Holdings Limited | Professional & scientific | 2006 |
| Ecsec Limited | Construction | 1979 |
| ED Dunlop Racing Ltd | Arts & recreation | 2006 |
| Edenbally (1) Limited | Real estate | 2023 |
| Edgcombe Construction Services Limited | Construction | 2019 |
| Edward F. Saltmarsh (Produce) Limited | Financial services | 1977 |
| Edward Prosser Limited | Information & communication | 2014 |
| Edward Stephens Physiotherapy Ltd | Health & social care | 2017 |
| Eezeemovers Ltd | Transport & logistics | 2023 |
| EF Consult Limited | Professional & scientific | 2016 |
| Egerton Propco 1 Limited | Real estate | 2025 |
| EIC Racing Ltd | Arts & recreation | 2022 |
| EJC Equine Services Ltd | Agriculture & fishing | 2026 |
| EK Ltd | Extraterritorial | 2023 |
| EKS GAS Services Ltd | Manufacturing | 2019 |
| EL Leisure Ltd | Wholesale & retail | 2018 |
| ELB Bloodstock Limited | Agriculture & fishing | 2024 |
| Electec Cambridgeshire Ltd | Administrative & support | 2016 |
| Electric Smiles Limited | Administrative & support | 2018 |
| Electrical Installation (Cambridge) Limited | Construction | 1961 |
| Elegance Nail & Beauty Ltd | Other services | 2024 |
| ELI G Construction Limited | Manufacturing | 2025 |
| Elite Caravans Limited | Wholesale & retail | 2001 |
| Elite Furniture Designs Limited | Manufacturing | 2009 |
| Elizabeth R Limited | Real estate | 2023 |
| Ellis House Limited | Real estate | 2021 |
| Elona Solutions Ltd | Wholesale & retail | 2023 |
| ELY Investors Ltd | Hospitality | 2020 |
| Elysian Charters Limited | Administrative & support | 1988 |
| Emberax Ltd | Wholesale & retail | 2026 |
| Emily Grass Limited | Other services | 2018 |
| Emma O'Gorman Limited | Arts & recreation | 2012 |
| Emma Spencer Limited | Information & communication | 2012 |
| Emporium Interiors Ltd | Construction | 2014 |
| Emvi Racing Ltd | Professional & scientific | 2023 |
| EMW Equine Ltd | Agriculture & fishing | 2025 |
| Energetic Butterfly Ltd | Extraterritorial | 2025 |
| Engaging Sport Limited | Professional & scientific | 2016 |
| Enhance the Face and Body Clinic Limited | Other services | 2015 |
| Entopy Limited | Professional & scientific | 2014 |
| Envalyx Ltd | Professional & scientific | 2026 |
| EO Limited | Extraterritorial | 2008 |
| EPB Consultancy Limited | Professional & scientific | 2020 |
| EPF Properties Limited | Construction | 2016 |
| Ephemeral Studio Limited | Wholesale & retail | 2021 |
| Epic Alva 1 Limited | Real estate | 2023 |
| Epic Alva 2 Limited | Real estate | 2024 |
| Epic Alva 3 Limited | Real estate | 2025 |
| Epic Alva 4 Limited | Real estate | 2025 |
| Epic Alva 5 Limited | Real estate | 2025 |
| Epic Staunton LLP | | 2012 |
| Eqmedic Ltd | Professional & scientific | 2017 |
| Equi-Ping Limited | Manufacturing | 2018 |
| Equiclean Ltd | Other services | 2025 |
| Equidi - T Limited | Professional & scientific | 2025 |
| Equilibre VET Ltd | Professional & scientific | 2022 |