| 1ST Serve Limited | Administrative & support | 2021 |
| 4 the Future Limited | Information & communication | 1998 |
| A Bird Consulting Limited | Information & communication | 2009 |
| A C Stevens Engineering Ltd | Professional & scientific | 2016 |
| AAL Transport Ltd | Transport & logistics | 2019 |
| AC Electrical Solutions (Commercial) Ltd | Construction | 2019 |
| AC Electrical Solutions (Gloucestershire) Limited | Construction | 2016 |
| Acacia Management Ltd | Professional & scientific | 2021 |
| Adoddle Ltd | Administrative & support | 2021 |
| Adoddle Management Ltd | Real estate | 2023 |
| Agriplan Cymru Limited | Professional & scientific | 2003 |
| Albero DI Olivo Limited | Wholesale & retail | 2010 |
| ALL About Doors Limited | Extraterritorial | 2020 |
| Alma House and Home Farm Management Limited | Households | 1989 |
| ALR Brokers Ltd | Financial services | 2021 |
| ALT ARB Ltd | Administrative & support | 2018 |
| Always Shine Limited | Administrative & support | 2022 |
| Amazingfindzuk Ltd | Wholesale & retail | 2020 |
| Areamanor Services Limited | Administrative & support | 1994 |
| ARI Trans Ltd | Transport & logistics | 2024 |
| ARP Group Services Ltd | Manufacturing | 2026 |
| ARP Installations Limited | Administrative & support | 2005 |
| Aviator Enterprises Ltd | Hospitality | 2018 |
| B & B Services Ltd | Construction | 2009 |
| Bagol Technologies Limited | Information & communication | 2025 |
| Barr Parks Limited | Hospitality | 2010 |
| Bentleys Castle Fruit Farm Ltd | Agriculture & fishing | 2013 |
| Best Logistic Solutions Ltd | Transport & logistics | 2024 |
| Bettee's CIC | Arts & recreation | 2026 |
| BG Plumbing & Heating Services Ltd | Construction | 2021 |
| BIZ Stationery Limited | Extraterritorial | 2019 |
| BIZ+ Stationery Superstores Limited | Wholesale & retail | 2012 |
| Bm&T European Restructuring Solutions Limited | Administrative & support | 2018 |
| Bockmer Investments Limited | Real estate | 1996 |
| Botanica Plant Centre Limited | Wholesale & retail | 2026 |
| Bower Power (Wyevale) Limited | Energy | 2014 |
| Bridgnorth Chimney Sweep Ltd | Administrative & support | 2016 |
| Broady's Hair & Beauty Ltd | Other services | 2021 |
| Brookside Caravan Servicing Ltd | Professional & scientific | 2022 |
| BUSSES4US CIC | Transport & logistics | 2023 |
| C & G Financial Consulting Ltd | Professional & scientific | 2017 |
| C & R Edwards Haulage Ltd | Transport & logistics | 2026 |
| C & R Rees Property Ltd | Real estate | 2021 |
| C & W Motors Ltd | Wholesale & retail | 2014 |
| C Rees Property Ltd | Real estate | 2021 |
| C.Prout Engineering Ltd | Professional & scientific | 2019 |
| Cape Agencies Limited | Wholesale & retail | 2020 |
| Carl Hester Limited | Agriculture & fishing | 2015 |
| Carlas Captivating Content Ltd | Professional & scientific | 2020 |
| Caterpeople Limited | Administrative & support | 2015 |
| Cgmppharma Ltd | Professional & scientific | 2018 |
| CH & GDS Properties Ltd | Real estate | 2020 |
| CHAMBER22 Ltd | Hospitality | 2024 |
| Charlotte Cafe Ltd | Hospitality | 2020 |
| Cheltenham Car Boot & Market Limited | Wholesale & retail | 2025 |
| Cheltenham DJS Ltd | Arts & recreation | 2022 |
| Churchill Associates (UK) Limited | Information & communication | 1990 |
| Cleeve Mill Business Park Ltd | Real estate | 2018 |
| Climatec Equipment Services Ltd. | Manufacturing | 2019 |
| Coalharbouruk Ltd | Wholesale & retail | 2025 |
| Cobblestones Carpentry Limited | Manufacturing | 2003 |
| Codecasket Ltd | Information & communication | 2019 |
| Collett Export Solutions Ltd | Wholesale & retail | 2020 |
| Collins Environmental Consultancy Limited | Professional & scientific | 2006 |
| Colour Catch Liquid Wraps Ltd | Manufacturing | 2016 |
| Compton Green Business Park LLP | | 2025 |
| Cool Beans Camper Van Hire Ltd | Administrative & support | 2021 |
| Core Incare Services Limited | Health & social care | 2017 |
| Cotswold Credit Limited | Financial services | 1985 |
| Court Consulting Services Limited | Real estate | 2002 |
| Couture Catering Limited | Wholesale & retail | 2025 |
| Coxmore Farm Ltd | Agriculture & fishing | 2022 |
| Create Newent CIC | Administrative & support | 2025 |
| D.H Carpentry and General Building Ltd | Construction | 2022 |
| David Gregory Design Ltd | Professional & scientific | 2008 |
| Debs Dusters Limited | Other services | 2016 |
| Delta Consulting London Ltd | Other services | 2020 |
| DIA Vision Limited | Other services | 2025 |
| Direct Autos Services Ltd | Wholesale & retail | 2024 |
| Direct Autos Servicing (Holdings) Limited | Professional & scientific | 2002 |
| Distruptive Art Ltd | Wholesale & retail | 2024 |
| Dlrf Limited | Construction | 2012 |
| Doctors Paycheck Ltd | Information & communication | 2023 |
| Drone Applications Ltd | Professional & scientific | 2023 |
| DS Designs Europe Ltd | Manufacturing | 2004 |
| Dudley Fencing & Landscaping Services Limited | Construction | 2024 |
| Easy Living Solutions Ltd | Other services | 2012 |
| Easyway Logistics Limited | Transport & logistics | 2020 |
| EBF Carpentry Limited | Construction | 2025 |
| Eckley Global Community School Ltd | Education | 2023 |
| Edge UK Developments Ltd | Agriculture & fishing | 2019 |
| Egis Consultancy Limited | Administrative & support | 2010 |
| Ehuk Enterprises Ltd | Hospitality | 2015 |
| Elmground Limited | Real estate | 2024 |
| Empowered Canine Physio Limited | Professional & scientific | 2019 |
| Enterprising IT Ltd | Information & communication | 2020 |
| Envoke Limited | Professional & scientific | 2011 |
| Erol's Sandwich Bar Ltd | Hospitality | 2025 |
| Essentially Cyber Limited | Information & communication | 2022 |
| Euroquip Newent Ltd | Manufacturing | 2007 |