| Dwell Well - Short Time Ltd | Hospitality | 2026 |
| Dynamic Hydraulics Limited | Professional & scientific | 2019 |
| E.H. Worlee & Co. (U.K.) Limited | Manufacturing | 1989 |
| E.P.S. Products Limited | Manufacturing | 1995 |
| Easy Living Retail Newcastle Ltd | Wholesale & retail | 2001 |
| Eccleshall Plumbing & Heating Limited | Construction | 2024 |
| Eco Smart Waste Group Limited | Water & waste | 2016 |
| Edenfield Property Developments Limited | Construction | 2022 |
| Edge Science Limited | Professional & scientific | 2019 |
| Eeshaanya Estates Limited | Real estate | 2023 |
| Eeshaanya Property Investments Limited | Real estate | 2022 |
| Effectus Construction Services Ltd | Construction | 2011 |
| Eggettes Limited | Hospitality | 2022 |
| EKA by Glowluxury Limited | Wholesale & retail | 2024 |
| EL Hend Limited | Real estate | 2022 |
| Elementum Management Limited | Other services | 2014 |
| Elena Healthcare Ltd | Health & social care | 2025 |
| Eleven Avenue Ltd | Wholesale & retail | 2015 |
| Elicio Global Limited | Professional & scientific | 2018 |
| Eljay Risk Management Limited | Professional & scientific | 2009 |
| Embroidery Training Limited | Information & communication | 2014 |
| EMS Industries Limited | Manufacturing | 1995 |
| Encompass Dance Academy Ltd | Education | 2024 |
| Endocrinology UK Ltd | Health & social care | 2017 |
| Endodiabe Physician Limited | Health & social care | 2017 |
| Enoch Care Ltd | Health & social care | 2020 |
| Entire Services Limited | Construction | 2016 |
| Envisage Properties Staffordshire Limited | Construction | 2024 |
| Equity Wealth Builders Limited | Real estate | 2017 |
| Euphonious Music Promotions Ltd | Information & communication | 2009 |
| Euro Printer Store Limited | Extraterritorial | 2019 |
| Eurostar Distribution Limited | Administrative & support | 2006 |
| Eurostar Global Electronics Limited | Information & communication | 2007 |
| Eurostar Global Limited | Information & communication | 2016 |
| Eurostar Group Holdings Limited | Financial services | 2018 |
| EV (Realisations) Limited | Arts & recreation | 1982 |
| Ev-Connected Ltd | Extraterritorial | 2020 |
| Evans and Lewis Ltd. | Financial services | 2011 |
| Evans Engine Services Limited | Wholesale & retail | 2024 |
| EX Astra Ltd | Professional & scientific | 2023 |
| F & Bailey Co Limited | Administrative & support | 2016 |
| F.A.B Systems (UK) Limited | Extraterritorial | 2002 |
| F.Mcguinness & Sons Limited | Wholesale & retail | 2018 |
| Fabhex Ltd | Manufacturing | 2025 |
| Factorytalk Ltd | Information & communication | 2020 |
| Fairfax Manor Care Ltd | Health & social care | 2021 |
| Farestone Limited | Hospitality | 2011 |
| Farrell Construction (Midlands) Limited | Construction | 2018 |
| Fastdeck Resins Limited | Construction | 2024 |
| Fenster Hardware Ltd | Wholesale & retail | 2007 |
| Fenster Property Services Limited | Real estate | 2019 |
| Fintech Digital Platforms Limited | Manufacturing | 2018 |
| Fishpix Limited | Arts & recreation | 2005 |
| Fitzgerald Properties Ltd | Real estate | 2014 |
| Five Fields Property Limited | Real estate | 2026 |
| Flemming Properties Limited | Real estate | 2022 |
| Flwstate Media Limited | Information & communication | 2025 |
| Focus Education Network Limited | Administrative & support | 2004 |
| Folkespeare Limited | Manufacturing | 2023 |
| Follwell Farming Limited | Administrative & support | 2006 |
| Forty Nine Investments Ltd | Real estate | 2026 |
| Foxtail Barns Ltd | Other services | 2021 |
| Foyer Leisure Limited | Administrative & support | 2019 |
| Francesca Mills Limited | Arts & recreation | 2021 |
| Freightspeed Limited | Transport & logistics | 2025 |
| Freshcomms UK Limited | Manufacturing | 2009 |
| Freshwater Properties Limited | Real estate | 2004 |
| Front Line Pumps & Systems Limited | Manufacturing | 2017 |
| Front ROW Audio Ltd | Information & communication | 2021 |
| Fruitcake Marketing Limited | Administrative & support | 2025 |
| Fuel Solutions (UK) Limited | Administrative & support | 2009 |
| Fuel Solutions Limited | Extraterritorial | 2010 |
| Funding Wall Limited | Information & communication | 2018 |
| Fyffes PEN Holdings Limited | Financial services | 2017 |
| G and B. Mccready Limited | Transport & logistics | 1969 |
| G E Shenton & Co Ltd | Professional & scientific | 2017 |
| G R B Holdings Ltd | Financial services | 2020 |
| G&E Fires, Stoves & Bathrooms Limited | Wholesale & retail | 2013 |
| G.C. Dewey & SON Limited | Manufacturing | 1964 |
| Gazzas Meal Preps Limited | Hospitality | 2020 |
| Geiger Handling (UK) Limited | Professional & scientific | 1998 |
| Gemini Asset Holdings Ltd | Financial services | 2021 |
| GF Gym Ltd | Wholesale & retail | 2023 |
| GG Building Contractors Limited | Construction | 2023 |
| Ghaesthetics Academy Ltd | Other services | 2024 |
| Ghaestheticsuk Ltd | Other services | 2022 |
| Gifted DJ Workshops Ltd | Education | 2010 |
| Gifted Workshops Community Interest Company | Education | 2009 |
| Gina Marie Hair and Beauty Limited | Other services | 2022 |
| Ginger People Europe Limited | Wholesale & retail | 2012 |
| Gisbourne Consultants Limited | Information & communication | 2012 |
| Global Purveyors Limited | Wholesale & retail | 2021 |
| Go Medical UK Limited | Wholesale & retail | 2012 |
| Golden OWL PUB Group Ltd | Hospitality | 2025 |
| Golf Marketing Hub Ltd | Professional & scientific | 2020 |
| Goodland Estate Limited | Real estate | 2025 |
| Goodwinuk Ltd | Construction | 2021 |
| Gorgeous Invites Limited | Arts & recreation | 2015 |
| Goto Live Limited | Other services | 2015 |
| Gough & Co (Engineering) Limited | Manufacturing | 2002 |