| Eaglet Management Ltd | Professional & scientific | 2019 |
| Easy Storage Technologies UK Limited | Information & communication | 2019 |
| Ebenezer Limited | Hospitality | 2021 |
| Ebenezer Trading Limited | Hospitality | 2017 |
| Eco Motors Limited | Wholesale & retail | 2013 |
| Edart Property Limited | Real estate | 2008 |
| Eddleston Investments Limited | Real estate | 2025 |
| Edge Landscapes Ltd | Administrative & support | 2025 |
| Edge Protection Federation | Other services | 2018 |
| Education for Life CIC | Education | 2022 |
| Edwards of Nantwich Limited | Hospitality | 2024 |
| Edwards Power Cleaning Limited | Water & waste | 2003 |
| Efficiency Holdings Limited | Financial services | 2024 |
| Egerton Northwest Ltd | Hospitality | 2019 |
| EGG Lane Development Company Limited | Construction | 2019 |
| EGW Holdings Ltd | Financial services | 2026 |
| EJ Collective Holdings Limited | Financial services | 2024 |
| EJ Munch Limited | Hospitality | 2024 |
| Ej's Cars Limited | Wholesale & retail | 2022 |
| EJB Floristry Limited | Wholesale & retail | 2023 |
| Eldon Place (Patricroft) Management Co. Limited | Extraterritorial | 2004 |
| Elevare Wellbeing Ltd | Health & social care | 2025 |
| Eleven Residential Limited | Professional & scientific | 2022 |
| Elismuir Limited | Wholesale & retail | 2020 |
| Elite Brand Solutions Ltd | Professional & scientific | 2025 |
| Elite Coachworks & Horseboxes Ltd | Manufacturing | 2013 |
| Elite Fitness (Cheshire) Limited | Arts & recreation | 2025 |
| Elite Home Entertainment Ltd | Wholesale & retail | 2001 |
| Elite Logos 4U Limited | Wholesale & retail | 2011 |
| Eljmc Ltd | Professional & scientific | 2024 |
| Ellie Louise Medical Aesthetics Ltd | Other services | 2025 |
| Ellumin Aesthetics Ltd | Health & social care | 2026 |
| Embertec Ltd | Construction | 2025 |
| Emerton Holdings Limited | Financial services | 2023 |
| Emerton Roofing (Western) Limited | Construction | 1962 |
| Emlych Limited | Professional & scientific | 2013 |
| Emma Brown Ltd | Other services | 2017 |
| Emray Industrial Services Limited | Wholesale & retail | 2020 |
| Encasita Limited | Hospitality | 2022 |
| Encompass Properties Limited | Construction | 2020 |
| Energyfit Ltd | Professional & scientific | 2013 |
| Engineering and Quality Services Ltd. | Construction | 2011 |
| ENH Services Limited | Professional & scientific | 2021 |
| Ensure Safety and Training Limited | Public administration | 2013 |
| Entira Ltd | Wholesale & retail | 2026 |
| Envirocare (NW) Limited | Water & waste | 2016 |
| Envirocraft Waste Management Ltd | Water & waste | 2019 |
| Envirocraft Waste Solutions Limited | Water & waste | 2011 |
| Envision IT Group Limited | Information & communication | 2026 |
| Envoy (Stoke) Ltd | Transport & logistics | 2010 |
| Enzo Commercial Solutions Limited | Hospitality | 2008 |
| Epos Crewe Limited | Other services | 2020 |
| EQ Matches Limited | Wholesale & retail | 2024 |
| Equilibrium Collective Limited | Real estate | 2021 |
| Equilibrium Financial Services Limited | Financial services | 2009 |
| Equimark Ltd | Administrative & support | 2003 |
| Equinordic PVT Ltd | Professional & scientific | 2018 |
| Escupudantay OV Ltd | Wholesale & retail | 2020 |
| Eskdale Road Ltd | Real estate | 2025 |
| Espa Engineering Limited | Professional & scientific | 2020 |
| European Spectrometry Services Limited | Manufacturing | 2013 |
| Euryan Limited | Information & communication | 2025 |
| Evaluate Supplier Management Portal Limited | Professional & scientific | 2016 |
| Ever Glorious Limited | Other services | 2025 |
| Evergreen Environmental Services Limited | Water & waste | 2015 |
| Evolve Animal Health Limited | Agriculture & fishing | 2025 |
| Evolve Together Limited | Professional & scientific | 2020 |
| Express 365 Ltd | Transport & logistics | 2025 |
| Extrashare Limited | Professional & scientific | 2002 |
| F & D Catering Ltd | Hospitality | 2012 |
| F E Mctigue Ltd | Administrative & support | 2023 |
| F-Tec Solutions Ltd | Wholesale & retail | 2014 |
| F.E.O. Shone and Sons Limited | Wholesale & retail | 2020 |
| F.J. Need (Foods) Limited | Hospitality | 2011 |
| FAB Apartments Ltd | Real estate | 2022 |
| Fabsal Ltd | Transport & logistics | 2016 |
| Faculty 55 Ltd | Professional & scientific | 2025 |
| Faira Limited | Construction | 2024 |
| Fall Arrest Safety Equipment Training | Other services | 2003 |
| Farmers PUB Co Ltd | Hospitality | 2025 |
| Farrington Contractors Limited | Construction | 2022 |
| Faset Training and Certification Ltd | Administrative & support | 2020 |
| Fedenergy Group Limited | Construction | 2025 |
| Fedenergy Limited | Extraterritorial | 2025 |
| Feetinneed Ltd | Health & social care | 2021 |
| Fellbrook Construction Ltd | Construction | 2007 |
| Ferguson Group Ltd | Wholesale & retail | 2021 |
| Fetita Limited | Health & social care | 2023 |
| Ffabriga Ltd | Construction | 2025 |
| FGA Limited | Administrative & support | 2002 |
| Filler Warehouse Ltd | Other services | 2022 |
| FINGERNAILS4CASH Ltd | Other services | 2025 |
| Finneys Landscaping Contractors Ltd | Administrative & support | 2024 |
| Fire Earth Alchemy Ltd | Other services | 2025 |
| Firmane Ltd | Transport & logistics | 2011 |
| First Class BID Services Limited | Professional & scientific | 2018 |
| Fisher Environmental Limited | Construction | 2004 |
| Fisher Environmental Sales Limited | Professional & scientific | 2014 |
| FIT4CULTURE Ltd | Arts & recreation | 2021 |
| Fixed Point Consulting Limited | Information & communication | 2015 |