| NO.1AESTHETICS-SKIN-LASER Ltd | Other services | 2026 |
| Noodles & Nimbus Limited | Wholesale & retail | 2025 |
| North END Nurseries Limited | Administrative & support | 2015 |
| North West Commercial Finance Ltd | Financial services | 2009 |
| Northern Power Services Limited | Construction | 2019 |
| Northstar Sports Management Ltd | Professional & scientific | 2020 |
| Northweave Advisory Ltd | Professional & scientific | 2026 |
| Northwich Plastics Limited | Wholesale & retail | 2018 |
| Notebox Limited | Financial services | 1998 |
| Nova Leisure Ltd | Arts & recreation | 2026 |
| Nudgme Ltd | Information & communication | 2026 |
| Nuthatch Construction Limited | Construction | 2022 |
| Nuthurst Gardens Management Co. Limited | Households | 1993 |
| Nutrition and Performance Coaching Ltd | Health & social care | 2025 |
| Nutsoft Consultants Limited | Information & communication | 2021 |
| Nuttall Property Group Ltd | Construction | 2019 |
| O Chantler Share Portfolio Limited | Financial services | 2025 |
| O Moody Property Investments Limited | Real estate | 2024 |
| Oak Tree Court (Poole) Management Company Limited | Households | 2006 |
| Oakes Corner Distillery Ltd | Manufacturing | 2024 |
| Oakes Millers Limited | Manufacturing | 1937 |
| Oaklands Equestrian Services Ltd | Agriculture & fishing | 2022 |
| Oasis Hair Nantwich Limited | Other services | 2015 |
| OAT Market Holdings Ltd | Real estate | 2013 |
| OCC Computer Personnel (Northern) Limited | Information & communication | 1978 |
| Oden Lounge Limited | Hospitality | 2023 |
| Oden Medical Limited | Health & social care | 2021 |
| OFF Piste Investments Ltd | Real estate | 2016 |
| Office Furniture Place Stoke Limited | Wholesale & retail | 2022 |
| OH My Glow Training Academy Ltd | Other services | 2023 |
| Ohara Accountants Limited | Professional & scientific | 2007 |
| Oikos Family Research Limited | Administrative & support | 2011 |
| Okapi Ventures Limited | Administrative & support | 2024 |
| Old Hall Storage Limited | Other services | 2021 |
| Old School Bodybuilding Prep Ltd | Education | 2025 |
| Olde Wyche Holdings Ltd | Financial services | 2025 |
| Oldskool Records UK Ltd | Information & communication | 2026 |
| Oliver Miles Chantler Investments Limited | Financial services | 2016 |
| Oliver Renewables Limited | Administrative & support | 2016 |
| Oliver Rose Property Ltd | Real estate | 2025 |
| OM Locum GP Ltd | Health & social care | 2022 |
| Omega Eyes Ltd | Health & social care | 2017 |
| Omni Stores Ltd | Wholesale & retail | 2022 |
| Omnia Technical Services Ltd | Construction | 2020 |
| ON the Grid Properties Ltd | Hospitality | 2015 |
| One Big Step Limited | Professional & scientific | 2011 |
| Onefic Limited | Real estate | 2024 |
| Only Scrans Group Ltd | Hospitality | 2025 |
| Ontek Systems Limited | Information & communication | 2026 |
| Ooddles Kitchen Ltd | Manufacturing | 2018 |
| Oops a Daisy Florist Limited | Wholesale & retail | 2014 |
| Opero Limited | Professional & scientific | 2014 |
| Optical 3D Limited | Professional & scientific | 2002 |
| Optimae Consulting Limited | Information & communication | 2018 |
| Optimagents Ltd | Wholesale & retail | 2019 |
| Optiprime Ltd | Health & social care | 2025 |
| Orbis Outdoor Adventures Ltd | Education | 2018 |
| ORE Brickwork Ltd | Construction | 2019 |
| Organixx Limited | Education | 2023 |
| Oriel Planning Ltd | Professional & scientific | 2021 |
| Origins Coffee Company Limited | Manufacturing | 2025 |
| Orion Instruments Limited | Manufacturing | 2019 |
| Orion Park (Crewe) Management Limited | Households | 2007 |
| Orka Scientific Ltd | Professional & scientific | 2018 |
| ORL Cheshire Limited | Health & social care | 2016 |
| Ornua Dairy Supply UK Limited | Wholesale & retail | 2020 |
| Ornua Ingredients Europe (UK) Limited | Manufacturing | 1985 |
| Ornua Ingredients UK Limited | Manufacturing | 1996 |
| Ornua Nantwich Limited | Manufacturing | 2016 |
| Ornua Nutrition Ingredients UK Limited | Wholesale & retail | 1989 |
| Osborn Knight Limited | Professional & scientific | 2013 |
| OSO FAB Ltd | Financial services | 2021 |
| Ostlers Nurseries Limited | Agriculture & fishing | 2003 |
| OUR Time Candles of Nantwich Limited | Wholesale & retail | 2021 |
| Outdoor FUN Limited | Health & social care | 2019 |
| Outperforming Limited | Real estate | 2025 |
| Oval Motorworks Limited | Wholesale & retail | 2024 |
| Overwater Marina Limited | Transport & logistics | 2009 |
| OZ Leisure Management Advisors Limited | Professional & scientific | 2025 |
| O’Hara Engineering Limited | Professional & scientific | 2024 |
| P & G Kelly Limited | Construction | 2002 |
| P and N Homes (Shavington) Limited | Construction | 2025 |
| P and R Homes (Cheshire) Ltd | Real estate | 2023 |
| P B Aerial Photography Ltd | Professional & scientific | 2019 |
| P Fabrics Limited | Wholesale & retail | 2024 |
| P M Powell Limited | Wholesale & retail | 2002 |
| P Turnock Limited | Agriculture & fishing | 2016 |
| P&N Homes (NW) Limited | Real estate | 2020 |
| P&N Investments (NW) Limited | Households | 2023 |
| P&S Papermill Services Limited | Manufacturing | 2013 |
| PA Mortgage Solutions Ltd | Financial services | 2022 |
| Pace Contract Flooring Limited | Construction | 2011 |
| Packaging One Holdings Limited | Administrative & support | 2025 |
| Packaging One Limited | Wholesale & retail | 2007 |
| Packsaddle Properties Ltd | Real estate | 2021 |
| Padel Boss UK Limited | Wholesale & retail | 2025 |
| Padel Marketing Limited | Professional & scientific | 2023 |
| Padelnorthwest Ltd | Arts & recreation | 2024 |
| PAG Cleaning Services (UK) Limited | Administrative & support | 2008 |
| Paje Consultancy Ltd | Professional & scientific | 2014 |