| ACP Solutions Limited | Manufacturing | 1996 |
| ADD Some Lemon Limited | Arts & recreation | 2020 |
| Agricultural and Mobile Airconditioning Limited | Energy | 1990 |
| Alan Clark Engineering 1991 Ltd | Manufacturing | 2019 |
| Allen Accountants (Nailsworth) Limited | Professional & scientific | 2024 |
| Amalfi Palace Ltd | Real estate | 2023 |
| AMC Skills Ltd | Professional & scientific | 2026 |
| Ashdown Garden School | Education | 2024 |
| Bhavani Wellness Limited | Health & social care | 2022 |
| Binary Electronics Limited | Other services | 1982 |
| Blackstone Ecology Ltd | Administrative & support | 2017 |
| Blandon Power Limited | Manufacturing | 2017 |
| Brookthorpe Engineering Limited | Manufacturing | 1999 |
| Built Labs Ltd | Information & communication | 2020 |
| C R Signs (Stroud) Limited | Manufacturing | 2012 |
| Cafe 53 Limited | Hospitality | 2005 |
| Capel 26 Holdings Limited | Financial services | 2025 |
| Creative Tech Consultant Ltd | Information & communication | 2025 |
| Cricklade Osteopaths 2020 Ltd | Health & social care | 2020 |
| David Denton FD Consultancy Limited | Professional & scientific | 2016 |
| Dermatopia Limited | Other services | 2020 |
| DR Jessica Prior Ltd | Other services | 2021 |
| E. a. Chamberlain LLP | | 2006 |
| Eagle Flight Training Ltd | Professional & scientific | 2015 |
| EBB & Flow Limited | Construction | 1997 |
| Ebley Coaches Limited | Transport & logistics | 2002 |
| Equator Natural Resources Ltd | Manufacturing | 2019 |
| Evie Software Limited | Information & communication | 2023 |
| Flocorn Ltd | Manufacturing | 2007 |
| Friends' Foreign Mission Association Trust Corporation(The) | Extraterritorial | 1903 |
| Gloucestershire Group Homes Limited | Health & social care | 1993 |
| Gouldings of Nailsworth Limited | Transport & logistics | 1984 |
| Greystar Developments Limited | Construction | 2014 |
| Growth Consultancy Ltd | Professional & scientific | 2006 |
| Heroh Energy Limited | Professional & scientific | 2016 |
| Heroh Enterprises Ltd | Construction | 2012 |
| Holy Spirits Ltd | Wholesale & retail | 2021 |
| Hoverkey Limited | Information & communication | 2012 |
| Kaas Distribution Ltd | Wholesale & retail | 2024 |
| KAC Cleaning Services Limited | Administrative & support | 2002 |
| Lady's Wood Shooting School Limited | Arts & recreation | 2013 |
| Laenus Ltd | Administrative & support | 2018 |
| LE Sommet Interiors Limited | Wholesale & retail | 2024 |
| Locks Mill Properties Limited | Real estate | 1942 |
| London House Management Company Limited | Real estate | 2005 |
| LW2013 (Retail) Limited | Wholesale & retail | 2016 |
| M Crowe Consulting Ltd | Professional & scientific | 2022 |
| Mayfly Developments Ltd | Construction | 2022 |
| Metsu Capital Limited | Financial services | 2025 |
| Monostudio Limited | Professional & scientific | 2004 |
| Mumford Mill Ltd | Real estate | 2022 |
| Nailsworth Accountancy & Bookkeeping Ltd. | Professional & scientific | 2000 |
| Nailsworth Lawn Tennis Club | Arts & recreation | 2010 |
| Ninja Hub Limited | Professional & scientific | 2014 |
| P,M.P.D. Builders Limited | Wholesale & retail | 1965 |
| Paradise Safaris Limited | Administrative & support | 2025 |
| Pearson Homes Limited | Construction | 2015 |
| PL James Ltd | Construction | 2011 |
| Playschool for Dogs Ltd | Other services | 2018 |
| Prestige Services (Cotswold) Ltd | Administrative & support | 2020 |
| Prospect Mews Residents Limited | Administrative & support | 2014 |
| Q L S Services Limited | Administrative & support | 2008 |
| Q S Holdings Limited | Manufacturing | 2017 |
| QMS Skills Limited | Professional & scientific | 2015 |
| Quaker International Educational Trust | Education | 1998 |
| Read for Good | Education | 2009 |
| Romaunce Ltd | Information & communication | 2017 |
| Room Business (Albion Chambers) Ltd | Construction | 2019 |
| Room Business Ltd | Construction | 2016 |
| Rowan Tree Centre for Health | Health & social care | 2024 |
| RT GP Services Ltd | Health & social care | 2019 |
| Ruskin Mill Centre for Practice | Education | 2020 |
| Ruskin Mill Land Trust Limited | Real estate | 1996 |
| Ruskin Mill Limited | Administrative & support | 1995 |
| Ruskin Mill Trust Limited | Education | 2010 |
| S.O.E. Consultants Limited | Financial services | 2007 |
| School of Larks CIC | Education | 2023 |
| Signetware Ltd | Information & communication | 2016 |
| Sky High (Oldstone) Ltd | Real estate | 2022 |
| Sky High Futures Limited | Construction | 2004 |
| Spring Mill Holdings Limited | Real estate | 1956 |
| Stroud Carpets Limited | Wholesale & retail | 2008 |
| Swift Storage Systems Ltd | Wholesale & retail | 2013 |
| Sylv's Engineering Ltd | Professional & scientific | 2024 |
| THE Acorn School Educational Trust Limited | Education | 2023 |
| THE Eco Accountancy Practice Limited | Professional & scientific | 2014 |
| THE Kitchen Angels (Glos) Ltd | Administrative & support | 2011 |
| THE Living Earth Land Trust | Administrative & support | 2009 |
| TIM Farmer Ltd | Professional & scientific | 2024 |
| Town & Country South West Ltd | Construction | 2023 |
| Tradeplas Conservatories Limited | Wholesale & retail | 2008 |
| Transform Residential Limited | Health & social care | 2010 |
| Trigonos Trading Limited | Hospitality | 2022 |
| Trinity Antiques Limited | Wholesale & retail | 2018 |
| Truturn Precision Engineering (Charfield) Limited | Manufacturing | 1986 |
| V4 Design Ltd | Information & communication | 2016 |
| Vinotopia Ltd | Wholesale & retail | 2011 |
| Vinotopia Storage Ltd | Wholesale & retail | 2024 |
| Woodford Publishing Ltd | Information & communication | 2020 |