| Edinburgh Car & Classic Online Ltd | Wholesale & retail | 2024 |
| Edinburgh Cupola Company Ltd | Construction | 2021 |
| Edinburgh Hackney CAB 633 Ltd. | Professional & scientific | 2006 |
| Edinburgh Innovation Park Hub Development Company Limited | Construction | 2021 |
| Edinburgh Innovation Park Hub Holdco Limited | Financial services | 2022 |
| Edinburgh Innovation Park Hub Operating Company Limited | Construction | 2022 |
| Edinburgh Innovation Park Joint Venture Company Limited | Professional & scientific | 2022 |
| Edinburgh Kitchen Install Ltd | Manufacturing | 2019 |
| Edinburgh Letting Limited | Real estate | 2024 |
| Edinburgh Modern Paving Limited | Construction | 2017 |
| Edinburgh Recycling Ltd | Water & waste | 2026 |
| Edinburgh Short Film Festival Ltd | Arts & recreation | 2013 |
| Edinburgh Waste Management EWM Limited | Water & waste | 2023 |
| EH1 Enterprise Ltd | Transport & logistics | 2025 |
| Eivissa Boutique Ltd | Other services | 2021 |
| EK Cleaning Services Ltd | Administrative & support | 2020 |
| EKI Limited | Construction | 2022 |
| Ekmn Investments Ltd | Real estate | 2017 |
| EKT Selection Ltd. | Information & communication | 2004 |
| Elcap | Health & social care | 1989 |
| Elevate Multisport Limited | Arts & recreation | 2024 |
| Elevenelevenglobal Ltd | Wholesale & retail | 2023 |
| Elite Access Ltd | Construction | 2026 |
| Elite Electrical Edinburgh Ltd | Construction | 2023 |
| Emerald Green Accountancy Services Ltd | Professional & scientific | 2020 |
| Enchanted Forest Nursery (Bishopbriggs) Ltd | Health & social care | 2013 |
| Enchanted Forest Nursery (Inverkip) Ltd | Health & social care | 2011 |
| Enchanted Forest Nursery Limited | Health & social care | 2005 |
| Enjoy East Lothian Limited | Arts & recreation | 2009 |
| Enjoy East Lothian Trading Limited | Arts & recreation | 2013 |
| Environmental Waste Systems Limited | Professional & scientific | 2002 |
| Equine Qualifications UK CIC | Professional & scientific | 2023 |
| Eriskay Ltd | Wholesale & retail | 2021 |
| Escara Hairdressing Ltd | Other services | 2022 |
| Eskmills Events Limited | Hospitality | 2025 |
| Eskmills Limited | Real estate | 2016 |
| Eskmouth Services Ltd | Arts & recreation | 2024 |
| Eskside Limited | Wholesale & retail | 2023 |
| Essonesson Limited | Professional & scientific | 2024 |
| ETI Lothian Ltd. | Construction | 1996 |
| Evante Web Ltd | Information & communication | 2025 |
| Everly Sports Ltd | Wholesale & retail | 2025 |
| EWM Recycling Ltd | Water & waste | 2024 |
| Exchange Bureau Limited | Financial services | 2017 |
| Expert Employment Law Limited | Professional & scientific | 2024 |
| Expert Radiology Limited | Health & social care | 2010 |
| Express Bread Ltd | Manufacturing | 2019 |
| F & W Wallyford Limited | Hospitality | 2017 |
| FAB Joinery & Maintenance Limited | Construction | 2020 |
| Fabulash Hair & Beauty Ltd | Other services | 2017 |
| Face 2 Face (Scotland) Limited | Administrative & support | 2014 |
| Fair Logistics Ltd | Transport & logistics | 2026 |
| Fairnbell Services Ltd | Professional & scientific | 2026 |
| Fanjango UK Limited | Information & communication | 2024 |
| FAT Tony's Limited | Hospitality | 2017 |
| Feachd Nàdair Ltd | Mining & quarrying | 2025 |
| Feline Devine Ltd | Other services | 2023 |
| Festival City Private Hire Cars Ltd | Transport & logistics | 2025 |
| Fidra Travel Ltd | Administrative & support | 2017 |
| Fine Finish Joinery Edinburgh Ltd | Construction | 2025 |
| Firechef Ltd | Wholesale & retail | 2015 |
| Firthgreen Limited | Transport & logistics | 2002 |
| Fisherrow Ltd | Arts & recreation | 2012 |
| Fisherrow PVT Limited | Wholesale & retail | 2025 |
| Fisherrow Stores Limited | Wholesale & retail | 2022 |
| Fishers Wynd Ltd | Hospitality | 2013 |
| Fitlicidad Ltd | Arts & recreation | 2024 |
| Five J Trading Ltd | Agriculture & fishing | 2026 |
| Fiùran Garden & Grounds Ltd | Administrative & support | 2026 |
| Fjdminvestments Ltd | Wholesale & retail | 2025 |
| Flag Hunter Services Ltd | Arts & recreation | 2025 |
| Fleming Smith Hairdressers Ltd | Other services | 2020 |
| Flip Cleaning Service Ltd | Administrative & support | 2025 |
| Floorworx Limited | Construction | 2025 |
| Flowercomb Ltd | Wholesale & retail | 2024 |
| FLR BIS Limited | Information & communication | 2018 |
| FM Fire Safety Solutions Limited | Professional & scientific | 2024 |
| Forge Minerals Limited | Professional & scientific | 2024 |
| Forrest Hepburn & Mcdonald Signs Ltd. | Manufacturing | 2000 |
| Forth Cleaning Services Ltd | Administrative & support | 2015 |
| Forth Lettings Limited | Households | 2025 |
| Fortuna IT Ltd | Information & communication | 2008 |
| Four Forty Consulting LLP | | 2014 |
| Four Seasons Health Care (Scotland) Ltd | Extraterritorial | 2019 |
| Fractal Factory Limited | Information & communication | 2026 |
| Francacia & Co Ltd | Hospitality | 2025 |
| Freelance Euro Services (Mdcxv) Limited | Professional & scientific | 2005 |
| G & G Wealth Limited | Financial services | 2014 |
| G D Plumbing & Heating Solutions Ltd | Professional & scientific | 2022 |
| G-Mak Refrigeration Services Limited | Construction | 2018 |
| G-Mak Services Scotland Ltd | Other services | 2025 |
| G.M.J Plumbing&Heating Ltd | Construction | 2024 |
| G1 Bodyshop Ltd | Wholesale & retail | 2023 |
| Galkot Ghumti Ltd | Real estate | 2018 |
| Game DR Limited | Information & communication | 2015 |
| Gellifique Nail Bar Ltd | Other services | 2024 |
| Gensource Group Ltd | Financial services | 2025 |
| Gensource Ltd | Professional & scientific | 2022 |
| Gentoo Project Management Ltd | Professional & scientific | 2025 |
| Geometry Projects Ltd | Construction | 2025 |