| Duffy Developments Limited | Real estate | 2021 |
| Dumbeck Cottage Limited | Other services | 2026 |
| Dumbeck Farmhouse Limited | Other services | 2026 |
| Dump IT Scotland Recycling Ltd | Water & waste | 2024 |
| Dunbar Haulage Ltd | Transport & logistics | 2004 |
| Dunbear EV Limited | Energy | 2026 |
| Dunlop Decorators Ltd | Construction | 2011 |
| Dunpath CIC | Health & social care | 2026 |
| Dunure Limited | Hospitality | 2021 |
| DUO Tech Recruitment Ltd | Administrative & support | 2025 |
| Dutu Spares Ltd | Wholesale & retail | 2025 |
| DWA Landscape Architects Limited | Professional & scientific | 2012 |
| DWD Developments (Scotland) Limited | Other services | 2025 |
| Dynamic Gymnastics | Arts & recreation | 2014 |
| Dynamo Essence Ltd | Wholesale & retail | 2026 |
| E & E Conglomerates Ltd | Professional & scientific | 2020 |
| E and D Scaffolding (Scotland) Limited | Construction | 2022 |
| E Mccormack Ltd | Administrative & support | 2026 |
| E P Bunce International Recovery Limited | Transport & logistics | 2022 |
| E&Y Property Development & Lettings Limited | Construction | 2025 |
| E.M.I Fibre Solutions Limited | Construction | 2026 |
| E.P.S.O.D Limited | Arts & recreation | 2020 |
| E02M Ltd | Wholesale & retail | 2026 |
| Easdale Healthcare Limited | Administrative & support | 2023 |
| East Deloraine Energy Limited | Energy | 2022 |
| Eastbrae Properties Limited | Real estate | 2005 |
| Eastend (Scotland) Limited | Hospitality | 2024 |
| Easterfield Investments Ltd | Financial services | 2020 |
| Easy Agent Ltd | Wholesale & retail | 2020 |
| Easycoda Ltd | Wholesale & retail | 2025 |
| EAV Installations Ltd | Professional & scientific | 2016 |
| EC Specialist Contractors Limited | Other services | 2021 |
| ECI Design Ltd | Professional & scientific | 2018 |
| Ecigarus NL Ltd | Wholesale & retail | 2025 |
| Eclipsed Ltd | Other services | 2023 |
| Eco Bazaar Ltd | Wholesale & retail | 2025 |
| Eco Electrics Scotland Ltd | Construction | 2018 |
| Eco Line Windows Ltd | Manufacturing | 2014 |
| Eco Renewables (Scotland) Ltd | Construction | 2024 |
| Ecomast Limited | Professional & scientific | 2019 |
| Ecomotive Renewables Ltd. | Construction | 2019 |
| Economic Development Solutions Limited | Professional & scientific | 2013 |
| Ecosse Inns Limited | Hospitality | 2017 |
| Edureka Consulting Ltd | Administrative & support | 2014 |
| Edwardson Digital Ltd | Professional & scientific | 2026 |
| Edzell Ltd | Administrative & support | 2026 |
| Eesa Traders Ltd | Wholesale & retail | 2020 |
| EG Murray Financial Planning Ltd | Financial services | 2012 |
| Egan Contracts Ltd | Construction | 2024 |
| Egcinspection Limited | Mining & quarrying | 2022 |
| Eisa Grocers Ltd | Wholesale & retail | 2025 |
| EJB18 Limited | Professional & scientific | 2020 |
| Elecnrg Ltd | Construction | 2025 |
| Electric Custom Gates Limited | Construction | 2023 |
| Elegant C&D Ltd | Other services | 2026 |
| Element 24-7 Limited | Construction | 2011 |
| Elimpus Limited | Professional & scientific | 2007 |
| Elite Beauty and Hair Ltd | Other services | 2014 |
| Elite Gears Driving School Ltd | Education | 2023 |
| Elite Gears Partners Limited | Education | 2025 |
| Elite Psychiatry Limited | Health & social care | 2021 |
| Elite Psychiatry Real Estate Limited | Real estate | 2025 |
| Elite Sports Analysis Limited | Arts & recreation | 2000 |
| Elite Transfer Group Ltd | Transport & logistics | 2026 |
| Elite Wellness Co Supplements Limited | Other services | 2024 |
| Eliteaura Limited | Wholesale & retail | 2024 |
| Elle Hair Studio Ltd | Other services | 2021 |
| Ellies Boutique [Scotland] Ltd | Wholesale & retail | 2022 |
| Ellum Joinery Ltd | Construction | 2026 |
| ELM Blue Ltd | Administrative & support | 2020 |
| Elmhurst Limited | Real estate | 1997 |
| Elvanfoot Energy Storage 2 Limited | Energy | 2022 |
| Elvanfoot Energy Storage 3 Limited | Energy | 2022 |
| Elvanfoot Energy Storage Limited | Energy | 2020 |
| Emerge Events Limited | Other services | 2026 |
| Emmanuel Best Care Ltd | Health & social care | 2018 |
| Empire State Limited | Real estate | 2008 |
| Empowered Employee Ltd | Arts & recreation | 2023 |
| Emtecs Scotland Ltd | Construction | 2021 |
| Endo 2 Limited | Hospitality | 2026 |
| Energy Assessors Holdings Ltd | Construction | 2025 |
| Energy Elite Ltd | Construction | 2024 |
| Energy HOW Ltd | Professional & scientific | 2018 |
| Energy Merchants (Wellington) Ltd | Energy | 2010 |
| Energy Plumbing and Heating Limited | Construction | 2025 |
| Enhance Properties (Rosebank) Limited | Other services | 2022 |
| Enigmatio Ltd | Information & communication | 2024 |
| Enviro Safe Scotland Ltd | Professional & scientific | 2025 |
| Environmental Key Fund Limited | Administrative & support | 2004 |
| Envy Beauty Lounge & Cosmetics Ltd | Other services | 2017 |
| Equals Advocacy Ltd | Health & social care | 2020 |
| Equans Specialist Contracting UK Limited | Construction | 1984 |
| Equi's Ice Cream (Motherwell) Ltd | Wholesale & retail | 2013 |
| Equilibrio Consulting Ltd | Administrative & support | 2025 |
| Equip Services Limited | Administrative & support | 2008 |
| ER Investment Solutions Ltd | Real estate | 2026 |
| ERL Classics Limited | Administrative & support | 2024 |
| ES100 Limited | Professional & scientific | 2026 |
| Esii Barbers Limited | Other services | 2021 |
| ESS ENN Travels Limited | Transport & logistics | 2013 |