| Spiritual Sojourn School Ltd | Information & communication | 2012 |
| Sports and Play Consulting Limited | Administrative & support | 2017 |
| Spotfood Moreton Ltd | Hospitality | 2025 |
| Spring Partners Ltd | Professional & scientific | 2020 |
| ST Peter's Church Daylesford Charitable Trust | Arts & recreation | 2014 |
| Stanleys Quarry Limited | Administrative & support | 2020 |
| Stanmoo Ltd | Professional & scientific | 2014 |
| Steve Laws Operations Ltd | Professional & scientific | 2021 |
| Stone Strength Weightlifting Limited | Arts & recreation | 2026 |
| Stonefern Court Management Limited | Households | 1990 |
| Storage Tank Hire Limited | Extraterritorial | 2008 |
| Storage Tank Sales Limited | Extraterritorial | 2008 |
| Storage Tank Services Limited | Other services | 2008 |
| Stowag Construction Limited | Construction | 2021 |
| Stride Logistics Ltd | Manufacturing | 2026 |
| Stuart Pell Opticians Limited | Wholesale & retail | 2004 |
| Studio by Tierney Ltd | Arts & recreation | 2025 |
| Studio Enemy Ltd | Professional & scientific | 2023 |
| Stuttgart Classica Limited | Wholesale & retail | 2019 |
| Subadra Limited | Construction | 1993 |
| Syngen Global Ltd | Professional & scientific | 2025 |
| S’Porter Management Limited | Wholesale & retail | 2014 |
| T&T Spittle Holdings Limited | Administrative & support | 2024 |
| Tabitha JK Limited | Wholesale & retail | 2011 |
| Tactical Services Limited | Construction | 2008 |
| Talent World Consulting Limited | Professional & scientific | 2022 |
| Tamaca Consulting Limited | Other services | 2019 |
| Tankcare (UK) Limited | Water & waste | 2011 |
| Tanser and Company Consulting Limited | Professional & scientific | 2006 |
| TAP Consultancy Ltd | Professional & scientific | 2023 |
| Tapani Ltd | Professional & scientific | 2024 |
| Tax Without Tears Ltd | Extraterritorial | 2004 |
| Tayabali Tomlin Cotswolds Limited | Extraterritorial | 2018 |
| Tayabali Tomlin Holdings Limited | Financial services | 2024 |
| Tayabali Tomlin Limited | Extraterritorial | 2018 |
| Tayabali Tomlin Probate Limited | Professional & scientific | 2020 |
| Taylor Direct Ltd | Wholesale & retail | 2022 |
| Tbos Global Limited | Extraterritorial | 2017 |
| Teach Back Ltd | Real estate | 2014 |
| Teacherbase Limited | Administrative & support | 2018 |
| Tengu Sake Limited | Wholesale & retail | 2012 |
| THE Authentic Consultancy Ltd | Information & communication | 2024 |
| THE Clerkenwell Brothers Limited | Professional & scientific | 2015 |
| THE Coordination Game Limited | Information & communication | 2024 |
| THE Cotswold Burger Company (CN) Ltd | Hospitality | 2024 |
| THE Cotswold Leisure Group Limited | Wholesale & retail | 2021 |
| THE Country Mouse Nursery Ltd | Education | 2016 |
| THE Crock Limited | Wholesale & retail | 2002 |
| THE Fairliving Movement Limited | Extraterritorial | 2022 |
| THE Fieldwork Collective Ltd | Professional & scientific | 2026 |
| THE Fosseway Practice Limited | Professional & scientific | 2018 |
| THE Hungry Griddle Ltd | Hospitality | 2026 |
| THE Last Company Limited | Administrative & support | 1986 |
| THE OH Collective Ltd | Wholesale & retail | 2020 |
| THE Old Bake House Totnes CIC | Hospitality | 2024 |
| THE Old Stores Toddington Limited | Real estate | 2025 |
| THE Perry Lithgow Partnership Limited | Administrative & support | 2003 |
| THE PR SET Limited | Professional & scientific | 2025 |
| THE PUB on the Hill Limited | Hospitality | 2024 |
| THE Shakespeare Brewing Company Limited | Extraterritorial | 2014 |
| THE Shakespeare Cider Company Limited | Extraterritorial | 2015 |
| THE Trollied PUB Company Limited | Hospitality | 2017 |
| Theraspace Services Ltd | Health & social care | 2025 |
| Thermal Consultancy Services Ltd | Professional & scientific | 2025 |
| Thrip Enterprises LLP | | 2012 |
| Tiggylicious Ltd | Wholesale & retail | 2021 |
| Time to Bake Limited | Other services | 2019 |
| TJK IP Limited | Professional & scientific | 2015 |
| Toast Group Ltd | Professional & scientific | 2015 |
| Toddlers Inn Ltd | Education | 2012 |
| TOO Many Things Ltd | Wholesale & retail | 2026 |
| Topaz Travel Limited | Administrative & support | 2025 |
| Total Home Environment Limited | Manufacturing | 2001 |
| Townsend Design Projects Ltd | Professional & scientific | 2025 |
| Townsend Global Ltd | Financial services | 2018 |
| Troopers Lodge Garage Limited | Wholesale & retail | 2019 |
| TW Woodall LLP | | 2010 |
| Twells Holdings Ltd | Real estate | 2022 |
| Twentythreeytl Limited | Other services | 2025 |
| Two Way Films Ltd | Arts & recreation | 2021 |
| Tyypo.App Ltd | Information & communication | 2025 |
| UK Business and Biodiversity Forum CIC | Other services | 2022 |
| Valhalla (U.K.) Ltd | Construction | 1971 |
| Vasco Maritime Services Ltd | Professional & scientific | 2015 |
| Victor Ludorum Ltd | Professional & scientific | 2017 |
| Victoria House Stow Limited | Real estate | 2017 |
| Vigilate Legal Limited | Professional & scientific | 2025 |
| Villa Plantae Ltd | Administrative & support | 2021 |
| VT Tower Limited | Extraterritorial | 2013 |
| VTP Development Management (London) Limited | Extraterritorial | 2017 |
| VTP London Limited | Extraterritorial | 2012 |
| W B Warwickshire Ltd. | Education | 2015 |
| W T Advertising Limited | Information & communication | 2019 |
| WA Cleaning Solutions Limited | Other services | 2016 |
| Warr & Mills Limited | Professional & scientific | 2019 |
| Warren Consultancy Ltd | Professional & scientific | 2025 |
| Watsonian Sidecars Limited | Manufacturing | 1967 |
| Watsonian Squire Limited | Manufacturing | 1988 |
| Waxology & Co Candles Limited | Wholesale & retail | 2025 |
| WD Developments (Cotswolds) Ltd | Construction | 2020 |