| DM Bear Ltd | Professional & scientific | 2019 |
| DMC Capital Limited | Professional & scientific | 2018 |
| Dmxp Productions Ltd | Information & communication | 2024 |
| Doorstep Milk Ltd | Manufacturing | 2019 |
| DOT on Technologies Limited | Information & communication | 2008 |
| Downie Drywall Systems Limited | Construction | 2000 |
| DR Kenneth Dawson Neurological Services Ltd | Health & social care | 2015 |
| Drivewithvovo Ltd | Health & social care | 2015 |
| Drybridge Garage Limited | Wholesale & retail | 2002 |
| Drybridge Vets Ltd | Professional & scientific | 2024 |
| DS Davies Ltd | Construction | 2024 |
| DS School of Music Limited | Education | 2016 |
| DSG Productions Limited | Arts & recreation | 2025 |
| Duke Street Investments Limited | Construction | 2004 |
| Duncan Mitchell Developments Ltd | Construction | 2012 |
| Duncan Mitchell Holdings Ltd | Financial services | 2023 |
| Dynamic K9 Ltd | Wholesale & retail | 2021 |
| E.J. Watkins Forest & ARB Ltd | Administrative & support | 2025 |
| Eagle Wharf Limited | Real estate | 2005 |
| Earley Engineering Limited | Manufacturing | 1993 |
| Earth to Action Ltd | Information & communication | 2024 |
| Eastofethan Limited | Information & communication | 2009 |
| Eathomas Ltd | Health & social care | 2020 |
| Ecology Practice Ltd | Professional & scientific | 2013 |
| Eddis Stores Limited | Wholesale & retail | 1964 |
| EDITION74 Consultancy Ltd | Professional & scientific | 2013 |
| EDS Forestry Ltd | Agriculture & fishing | 2013 |
| Edwards of Monmouth Limited | Construction | 1985 |
| Eezyhitch Limited | Manufacturing | 2010 |
| Eldrix Ltd | Information & communication | 2014 |
| Electrical Maintenance Services Ltd | Manufacturing | 2021 |
| Element Urbanism LLP | | 2015 |
| Elif Atug Ltd | Hospitality | 2017 |
| Elite Leasing Solutions Ltd | Administrative & support | 2025 |
| Elizogen Properties Limited | Real estate | 2023 |
| Ellaton Properties Limited | Construction | 2022 |
| Elliott Pardington Architecture & Design Limited | Professional & scientific | 2013 |
| Elro Properties (Caerwent) Limited | Real estate | 2026 |
| Elumio Limited | Information & communication | 2023 |
| Emergensys Consulting Limited | Professional & scientific | 2022 |
| Emerging Brands Limited | Wholesale & retail | 2008 |
| Empty Space CIC | Arts & recreation | 2024 |
| Enaid Wellbeing Ltd | Health & social care | 2024 |
| ENS Legis Limited | Extraterritorial | 2014 |
| EP Partnerships Limited | Professional & scientific | 2022 |
| Epic Theatre Limited | Arts & recreation | 2025 |
| Episode Two Ltd | Arts & recreation | 1994 |
| Equilibrium Property Limited | Real estate | 2014 |
| Eric Charles Hairdressing Supplies Limited | Wholesale & retail | 2003 |
| Ernest Property Limited | Real estate | 2021 |
| Estee Property Management Ltd | Real estate | 2016 |
| Ether Corporation Ltd | Information & communication | 2020 |
| European Chow GAR Organisation Ltd | Education | 2025 |
| EV Bay Marking Co. Limited | Construction | 2023 |
| EV Groundwork Solutions Limited | Construction | 2023 |
| Ev-Lupin Ltd | Professional & scientific | 2014 |
| EVA & Co Solutions Limited | Administrative & support | 2025 |
| Evanssons Limited | Construction | 2007 |
| Execprova Virtual Assistant Services Ltd | Administrative & support | 2019 |
| Exercens Limited | Professional & scientific | 2007 |
| Exponent IP Ltd | Professional & scientific | 2008 |
| EYE4DESIGN Construction Limited | Construction | 2016 |
| EYE4DESIGN Maintenance Limited | Construction | 2017 |
| F & F United Limited | Construction | 2016 |
| Faded Consulting Limited | Administrative & support | 2022 |
| Falcon Court Holdings Limited | Financial services | 2018 |
| Falcon Takeaway Ltd | Hospitality | 2025 |
| Fancy Freds Limited | Wholesale & retail | 2009 |
| Farriers Roaming Office Ltd | Information & communication | 2026 |
| FD Cosmetics Ltd | Other services | 2026 |
| FD Entertainments Ltd | Arts & recreation | 2008 |
| Feathertouch Hygienist Limited | Health & social care | 2014 |
| Fendy Builder Ltd | Construction | 2026 |
| FF London DLD Ltd | Professional & scientific | 2025 |
| FFB Properties Limited | Real estate | 2023 |
| Field Notes / Nodiadau Maes CIC | Education | 2025 |
| Fine & Country (Chepstow) Ltd | Real estate | 2022 |
| Finley K Financial Ltd | Financial services | 2017 |
| Firmtrack Limited | Professional & scientific | 2023 |
| First Occupational Health Ltd | Health & social care | 2021 |
| First Sight Design Limited | Arts & recreation | 2002 |
| FJ Civils Limited | Construction | 2019 |
| FJC Hire Limited | Administrative & support | 2026 |
| Flatjive Limited | Information & communication | 2025 |
| Flexi-Print Ltd | Manufacturing | 2019 |
| Flexible Vehicle Movements Limited | Transport & logistics | 2025 |
| FOR Fabric's Sake Ltd | Wholesale & retail | 2025 |
| Ford James Building & Roofing Ltd | Construction | 2024 |
| Forest Adventure Limited | Arts & recreation | 2000 |
| Forest and WYE Consultancy Ltd | Information & communication | 2025 |
| Forest Retreat Cottages Ltd | Hospitality | 2026 |
| Forgeside Developments Ltd | Construction | 2015 |
| Formentia Ltd | Professional & scientific | 2025 |
| Formula Purchasing Limited | Professional & scientific | 2002 |
| Forte Music Distribution Limited | Wholesale & retail | 2005 |
| Forte Travel Management Limited | Professional & scientific | 2012 |
| Foundat Limited | Financial services | 1999 |
| FOUNDRY46 Limited | Hospitality | 2018 |
| Francline Properties Limited | Real estate | 1995 |
| Frank Brands Limited | Professional & scientific | 2010 |