| 1ST Day Limited | Professional & scientific | 2018 |
| 3TECH Rope Access Ltd | Administrative & support | 2018 |
| Agridigm Business Consultants Ltd | Administrative & support | 2016 |
| Albassinia Ltd | Hospitality | 2024 |
| Alfie’S Dream for Greyhounds Ltd | Administrative & support | 2016 |
| ALL Spark Electrical (Dumfries) Limited | Construction | 2021 |
| Arpexas (Scotland) Limited | Professional & scientific | 2014 |
| Autiq AI Limited | Extraterritorial | 2025 |
| Autiq Limited | Professional & scientific | 2025 |
| Axjura Limited | Financial services | 2024 |
| Bankfoot House (Moffat) Ltd. | Health & social care | 1964 |
| Barker Farms Limited | Agriculture & fishing | 1994 |
| Beechwood Retreat Ltd | Hospitality | 2022 |
| Birthday Bear Soft Play Hire Ltd | Hospitality | 2022 |
| C&S Inspections Ltd | Other services | 2024 |
| Cairndale Ltd | Administrative & support | 2008 |
| Capitum Nature Limited | Agriculture & fishing | 2025 |
| Carlton Engineering Limited | Professional & scientific | 2016 |
| Champion International Products Limited | Professional & scientific | 2009 |
| Chantillie House Limited | Wholesale & retail | 2012 |
| Chefs in Scotland Limited | Administrative & support | 2006 |
| Cluckhen Brilliant Limited | Wholesale & retail | 2011 |
| Colin Picken Eyecare Limited | Health & social care | 2014 |
| COST2BUILD Trade Windows Ltd | Wholesale & retail | 2018 |
| Covta Ltd | Real estate | 2017 |
| Creative Placemakers CIC | Arts & recreation | 2025 |
| Crofthead Rentals Ltd. | Real estate | 2021 |
| CW Chen Ltd | Hospitality | 2024 |
| D J Livestock | Agriculture & fishing | 2025 |
| Danny Ross Scotland Limited | Administrative & support | 2002 |
| Dark Sky Spirits Ltd | Manufacturing | 2017 |
| Davidson Fencing Contractors Ltd | Agriculture & fishing | 2012 |
| Davies Forestry and Wildlife Management Ltd | Agriculture & fishing | 2023 |
| Deburgh Executive Management Limited | Professional & scientific | 1998 |
| Devon Property Services Ltd. | Other services | 2003 |
| Direct Vehicle Rentals (Scotland) Limited | Administrative & support | 2003 |
| Feel Good Farming Company Ltd | Wholesale & retail | 2026 |
| GAS2HEAT.COM Ltd | Construction | 2022 |
| GDP Marketing Services Limited | Information & communication | 2015 |
| George Hunter & SON Limited | Construction | 2015 |
| Grey Mare Finance Limited | Professional & scientific | 2025 |
| Hameknowe Ltd | Administrative & support | 2023 |
| Hartfell Hill Running Club | Arts & recreation | 2018 |
| Heat Central Ltd. | Other services | 2006 |
| Hello?Fire Ltd | Professional & scientific | 2022 |
| Hestyngate Ltd | Agriculture & fishing | 2025 |
| Ianthe Boutique Ltd | Wholesale & retail | 2017 |
| Ignis Automatic Fire Suppression Systems Limited | Administrative & support | 2018 |
| Impact for Health International Ltd | Professional & scientific | 2022 |
| Industrial Safety Solutions Limited | Professional & scientific | 2015 |
| Integrity Support Solutions Group Limited | Mining & quarrying | 2004 |
| International Coaching Psychotherapy Ltd | Other services | 2026 |
| J B R Projects Ltd | Professional & scientific | 2014 |
| James Wheatcroft Limited | Real estate | 2015 |
| JKM Business Services Ltd | Professional & scientific | 2015 |
| Kayantics Education Ltd | Education | 2025 |
| Kofeopia Ltd | Manufacturing | 2024 |
| Laird Lettings Ltd | Real estate | 2025 |
| LJC Moffat Plant Hire Ltd | Construction | 2015 |
| Lutton & Co. Ltd | Agriculture & fishing | 1990 |
| M Rankin Plant Limited | Construction | 2022 |
| Make/Change Collective Community Interest Company | Education | 2022 |
| Maltas Farms (Scotland) Holdings Limited | Extraterritorial | 2015 |
| Malyon Homes Ltd. | Hospitality | 2016 |
| Malyon Property Ltd. | Hospitality | 2016 |
| Manse Interiors Limited | Wholesale & retail | 2021 |
| Mark One Telecoms Ltd | Construction | 2023 |
| Moffat Dental Practice Ltd | Health & social care | 2021 |
| Moffat Golf Clubhouse Ltd | Hospitality | 2022 |
| Moffat Hotel Group Ltd | Hospitality | 2024 |
| Moffat Print Ltd | Manufacturing | 2024 |
| Moffat Town Hall Redevelopment Trust | Real estate | 2008 |
| Municipal and General Vehicle Contracts Limited | Real estate | 1999 |
| Mutchies Munchies Ltd | Hospitality | 2018 |
| NB Events Management Limited | Administrative & support | 2025 |
| Neilson Electrical Services Limited | Construction | 2014 |
| Nithsdale Vending Limited | Manufacturing | 1988 |
| NMF HV Engineering Ltd | Manufacturing | 2025 |
| North West Services (Scotland) Ltd | Construction | 2017 |
| P.R Decorators Ltd | Construction | 2018 |
| Pasta Palette Ltd | Manufacturing | 2024 |
| Pocket Mountains Limited | Information & communication | 2002 |
| Pqrm Ltd | Professional & scientific | 2012 |
| Productive Placemakers Ltd | Professional & scientific | 2025 |
| PYP 1 Limited | Financial services | 2018 |
| R Matchett & SON Ltd | Construction | 2014 |
| R.G.L. Homes (Moffat) Limited | Construction | 2003 |
| Reform South Ltd | Administrative & support | 2026 |
| RGL Plant (Moffat) Ltd. | Construction | 1995 |
| RM Fitness Nutrition Ltd | Arts & recreation | 2022 |
| RS Samra Ltd | Hospitality | 2022 |
| Russell Murray Ltd | Hospitality | 2026 |
| Ryder Services Limited | Construction | 2008 |
| Scottish National FAT Stock Club | Agriculture & fishing | 2009 |
| Selcoth Forestry Ltd | Agriculture & fishing | 2021 |
| Simmons Bilt Clothing Company Ltd | Manufacturing | 2012 |
| Stephen Sanderson Limited | Administrative & support | 2012 |
| Systemlinks Associates Limited | Professional & scientific | 2015 |
| T R F Contracts Limited | Construction | 2021 |
| T.A.V. Investments Limited | Real estate | 2001 |