| ABB Services Ltd | Real estate | 2024 |
| AJW Control Solutions Ltd | Information & communication | 2015 |
| Alex & Alisia Company Limited | Hospitality | 2023 |
| ALL Fleet Services Limited | Administrative & support | 2003 |
| Anjajavy Consulting Limited | Professional & scientific | 2015 |
| Appia Ltd | Information & communication | 2025 |
| Arain Futures Ltd | Professional & scientific | 2026 |
| Auraphonic Limited | Information & communication | 2014 |
| AVE2 Limited | Wholesale & retail | 2025 |
| BD Building Solutions LLP | | 2011 |
| Beard & Tandy Developments Ltd | Construction | 2012 |
| Beezii Ltd | Transport & logistics | 2019 |
| Books for Bugs Limited | Wholesale & retail | 2022 |
| Bordello Banquets and Rentals Limited | Hospitality | 1999 |
| Branna Holdings Limited | Financial services | 2021 |
| Bright Green Consulting Services Limited | Information & communication | 2007 |
| Brook House Accounting Services Limited | Professional & scientific | 2012 |
| Buaine Ltd | Construction | 2020 |
| C R Engineering Hereford Ltd | Professional & scientific | 2024 |
| Cadfan Limited | Manufacturing | 1996 |
| Catechxis Ltd | Professional & scientific | 2020 |
| Celtic Cattery Limited | Hospitality | 2002 |
| Cloverleaf Car Services Limited | Other services | 2005 |
| Colossal Building Ltd | Construction | 2019 |
| Conway Traders Limited | Water & waste | 2025 |
| Dean Farm Animal Sanctuary Limited | Real estate | 2016 |
| Dean Healthcare South West Ltd | Administrative & support | 2005 |
| Developing Commercial Excellence Ltd | Professional & scientific | 2015 |
| DMP Building Services Ltd | Construction | 2025 |
| Duck and Barrel Limited | Information & communication | 2026 |
| Eden's Cave CIC | Education | 2025 |
| F M Electronics Ltd | Extraterritorial | 2021 |
| Fisher Cleaning Ltd | Administrative & support | 2024 |
| FM Security Ltd. | Administrative & support | 1988 |
| Forest Air and Heat Ltd | Construction | 2026 |
| Forest Engineering Ltd | Professional & scientific | 2021 |
| Forest Gate Church | Other services | 2012 |
| Forest Huts Ltd | Manufacturing | 2021 |
| Forest Marquees Limited | Administrative & support | 2016 |
| Forest of Dean Fasteners Limited | Wholesale & retail | 2003 |
| Forest of Dean Trust | Education | 2011 |
| Forest Property Lettings Ltd | Real estate | 2022 |
| Forwood Enterprise Limited | Real estate | 1995 |
| Frangus Limited | Professional & scientific | 2005 |
| Fromthespot Limited | Information & communication | 2022 |
| Fudge Hairstyles Ltd | Other services | 2020 |
| Gardner Clayson Limited | Professional & scientific | 2026 |
| Gems Workflow Limited | Administrative & support | 2020 |
| Glassy Productions Limited | Professional & scientific | 2024 |
| Global Publishing Solutions Ltd | Information & communication | 2007 |
| H&N Real Estate Limited | Real estate | 2016 |
| Hair Systems Bristol Limited | Other services | 2025 |
| HAM & HAM Ltd | Arts & recreation | 2023 |
| Henryka Limited | Wholesale & retail | 2006 |
| Highview Cable Solutions Limited | Manufacturing | 2010 |
| Home Town Housing Limited | Real estate | 2023 |
| HSB Retail Ltd | Wholesale & retail | 2025 |
| Hybridstores Ltd | Wholesale & retail | 2018 |
| Infinity Skills LLP | | 2017 |
| ITS SO Media Limited | Information & communication | 2023 |
| Jane Acton PR Limited | Administrative & support | 2017 |
| Jenkins Home Improvements Ltd | Construction | 2021 |
| JSL Cleaning Ltd | Administrative & support | 2024 |
| JSL Property Projects Ltd | Construction | 2026 |
| JT Braid Ltd | Agriculture & fishing | 2025 |
| JW Property Lettings Limited | Real estate | 2021 |
| K Alder Electrical Limited | Construction | 2020 |
| Keen Thinking Limited | Administrative & support | 2000 |
| Kevin Burford Limited | Construction | 2002 |
| L2J2 Ltd | Information & communication | 2018 |
| LEL Scaffolding Limited | Construction | 2012 |
| Lesters Commercials Ltd | Manufacturing | 2018 |
| Let's Talk Well | Health & social care | 1994 |
| Lightspeed Media Limited | Arts & recreation | 2017 |
| M Seaborn Consulting Ltd | Financial services | 2024 |
| MAX Hyde Ltd | Information & communication | 2013 |
| Metric Company (UK) Limited | Manufacturing | 2002 |
| Mgaccounts Limited | Professional & scientific | 2010 |
| MH Perry Limited | Professional & scientific | 2003 |
| Mike Dyson Consultancy Ltd | Information & communication | 2018 |
| Millhall Consultants Ltd | Professional & scientific | 2000 |
| Mitcheldean Area Community Enterprise CIC | Health & social care | 2025 |
| Mitcheldean Catering Limited | Hospitality | 2026 |
| Mitcheldean Soap Ltd | Manufacturing | 2008 |
| Module IT Limited | Information & communication | 2007 |
| Monorails UK (Ross) Ltd | Professional & scientific | 2024 |
| Monument Geomatics Ltd | Professional & scientific | 2009 |
| Morgatronic Limited | Manufacturing | 2008 |
| Nelson Honey UK Limited | Manufacturing | 1997 |
| Newgen Knowledgeworks UK Limited | Administrative & support | 2014 |
| Odin Security Services Limited | Other services | 2019 |
| OMG Foods Limited | Manufacturing | 2019 |
| Outsyda Ltd | Administrative & support | 2024 |
| PG & CP Whitehouse Limited | Agriculture & fishing | 2013 |
| Phoenix Specialist Recruitment Ltd | Administrative & support | 2018 |
| Phonetica Limited | Information & communication | 2019 |
| Pinot Properties Ltd | Real estate | 2025 |
| Play Wooden CIC | Manufacturing | 2017 |
| Purozo Limited | Administrative & support | 2018 |
| Purple Factors Limited | Financial services | 2006 |