| 18 Carisbrooke Road Company Limited | Extraterritorial | 1986 |
| BIO Services Ltd | Transport & logistics | 2016 |
| C M Stevens Limited | Agriculture & fishing | 2017 |
| Cambridge 2030 CIC | Information & communication | 2020 |
| Cambridge BED and Breakfast Ltd | Hospitality | 2018 |
| Cambridge Centre for International Research Ltd | Education | 2020 |
| Cambridge Global Intelligence Ltd | Information & communication | 2025 |
| Capture Technologies Limited | Wholesale & retail | 1989 |
| Clockwork Audio Ltd | Arts & recreation | 2025 |
| Content Catalyst Limited | Information & communication | 2003 |
| Corvasave Limited | Real estate | 1992 |
| Craig S Design Ltd | Information & communication | 2018 |
| DELTA2020 LLP | | 2009 |
| DPN Technology Services Limited | Information & communication | 2026 |
| FBN Productions, Inc. | | 1991 |
| Forge Metal Recycling Limited | Water & waste | 2015 |
| Giovana Limited | Transport & logistics | 2019 |
| Hangar 16 Ltd | Information & communication | 2015 |
| Holdway Electrical Services Ltd | Construction | 2022 |
| IDF Industrial Doors Fabrication Ltd | Manufacturing | 2024 |
| Journey With Taste Limited | Arts & recreation | 2005 |
| KLN Facades Ltd | Construction | 2019 |
| KMB Maintenance Limited | Construction | 2016 |
| L M Roofing Ltd | Construction | 2013 |
| Lingos-Webb Family LLC | | 2023 |
| Long Range Systems PLC | Professional & scientific | 2004 |
| Longrange Systems UK Ltd | Information & communication | 1989 |
| Lovebeer Ltd | Manufacturing | 2017 |
| Mamma's Italian Street Food Ltd | Hospitality | 2024 |
| Mclaren Coutts Ltd | Information & communication | 2020 |
| Midsummer Energy Ltd | Wholesale & retail | 2010 |
| Milton Garden Products Limited | Wholesale & retail | 1989 |
| P & Q International Ltd | Information & communication | 1979 |
| Pavilion Doors Limited | Professional & scientific | 2018 |
| Phoenix Residential Developments Limited | Real estate | 2016 |
| PKD Group Limited | Financial services | 2015 |
| Prodromic Ltd | Professional & scientific | 2024 |
| Pure Civil Engineering Limited | Construction | 2016 |
| Quantum Espresso Foundation | Professional & scientific | 2011 |
| R & L Products Limited | Professional & scientific | 2003 |
| Red Balloon Learner Centre Cambridge | Education | 2017 |
| Rpkpub Limited | Hospitality | 2025 |
| Shorthand (UK) | | 2014 |
| Shreebeen Limited | Other services | 2025 |
| Sona Management Services Ltd | Professional & scientific | 2003 |
| South Oxford Accountancy Ltd | Professional & scientific | 2024 |
| Steele Solar Solutions Limited | Administrative & support | 2016 |
| Stop Corrosion Ltd | Agriculture & fishing | 2020 |
| T R Steele Contracts Limited | Agriculture & fishing | 2016 |
| THE Grayhunt Group Ltd | Financial services | 2021 |
| THE Phoenix Trust (Milton) Ltd | Health & social care | 2006 |
| THE Young Darwinian Ltd | Information & communication | 1999 |
| Wroxham Ludgate Cottage Limited | Hospitality | 2022 |
| Ziphii Limited | Information & communication | 2018 |