| 100001 Limited | Real estate | 2015 |
| 10002 Limited | Real estate | 2015 |
| Aspirecrm Ltd | Information & communication | 2016 |
| B&J Arora Ltd | Other services | 2026 |
| Bell Constructs Limited | Construction | 2011 |
| Blandscapes Limited | Administrative & support | 2017 |
| Bodyy Aesthetics Ltd | Health & social care | 2024 |
| Building Site Services Limited | Professional & scientific | 2001 |
| Ckcarpentryjoinery Ltd | Manufacturing | 2020 |
| Cromwell Properties (Derby) Limited | Real estate | 2018 |
| Cromwell Welding Limited | Construction | 2002 |
| Donald Hamilton LLP | | 2022 |
| Groundco Ltd | Construction | 2024 |
| Grow & Partners Limited | Professional & scientific | 2015 |
| Healthwatch Derbyshire Ltd | Health & social care | 2013 |
| Homecare Incorporated Limited | Extraterritorial | 2009 |
| Hull-Carter Estates Ltd | Real estate | 2022 |
| Icare Incorporated | Other services | 2011 |
| Kara Milford Limited | Other services | 2025 |
| Kingpin Management Services Limited | Administrative & support | 2012 |
| Land Survey Construction Ltd | Construction | 2005 |
| Land Survey Services Ltd | Professional & scientific | 2005 |
| Lucks Yard Clinic Ltd | Health & social care | 2026 |
| LWH Bookkeeping and Accountancy Services Ltd | Professional & scientific | 2025 |
| Milford Community Development Association Limited | Administrative & support | 2001 |
| Milford Kitchens Limited | Manufacturing | 2019 |
| Milford Pre-School | Education | 2005 |
| Milford Properties Limited | Professional & scientific | 2010 |
| Orpheus Music Ltd | Education | 2018 |
| PJC Software Solutions Ltd | Extraterritorial | 2013 |
| PMP Transport (Ireland) Ltd | Transport & logistics | 2005 |
| Premier Care Armagh Ltd | Health & social care | 2007 |
| Rowbridge (UK) Limited | Wholesale & retail | 2002 |
| SOS Fuel and Tankers Limited | | 2003 |
| ST Johns Street RTM Company Limited | Households | 2020 |
| T R White & SON Limited | Manufacturing | 2018 |
| Trade Secret Stock Care Ltd | Manufacturing | 2021 |
| Twentyone DPI Ltd | Arts & recreation | 2013 |
| Youbrella Limited | Professional & scientific | 2017 |
| Zeus Energies Limited | Professional & scientific | 2024 |