| Elyntrix Ltd | Information & communication | 2025 |
| Elzeon Group Ltd | Wholesale & retail | 2024 |
| EM Gurus Limited | Information & communication | 2024 |
| EM Immobilier Marrakech Ltd | Real estate | 2026 |
| EM P.I.E Ltd | Information & communication | 2026 |
| Emaize Innovation Limited | Manufacturing | 2025 |
| Emarkss Ltd | Information & communication | 2025 |
| Ember Aesthetics Limited | Other services | 2025 |
| Ember Healthcare Solutions Ltd | Health & social care | 2025 |
| Ember Services Limited | Administrative & support | 2026 |
| Emberwell Ltd | Manufacturing | 2025 |
| Embo Mains Farm Limited | Wholesale & retail | 2020 |
| EMC Essex Ltd | Real estate | 2025 |
| Emcalia Limited | Wholesale & retail | 2025 |
| Emerald Internet Solutions Limited | Information & communication | 2008 |
| Emerald Zenith Ltd | Agriculture & fishing | 2024 |
| Emerduit Limited | Wholesale & retail | 2026 |
| Emergence Publishing Limited | Manufacturing | 2025 |
| Emga Global Limited | Wholesale & retail | 2025 |
| Emlcom Ltd | Wholesale & retail | 2024 |
| Emlot Limited | Professional & scientific | 2014 |
| Emma Edison Lights Ltd | Wholesale & retail | 2025 |
| Emma Husband Financial Planning Ltd | Professional & scientific | 2024 |
| Emmahann Ltd | Wholesale & retail | 2020 |
| Emmanuel Rose Properties Ltd | Real estate | 2026 |
| Emmaside Solutions Ltd | Wholesale & retail | 2024 |
| Empire Aircraft Services Limited | Transport & logistics | 2014 |
| Empire Software Ltd | Information & communication | 2023 |
| Empowered Coaching Ltd | Education | 2026 |
| Empowered Physiotherapy Ltd | Other services | 2023 |
| Emprisc Properties Ltd | Real estate | 2025 |
| EMS Gains Ltd | Arts & recreation | 2024 |
| EN Art Gallery Ltd | Construction | 2019 |
| Enablatross Ltd | Administrative & support | 2025 |
| Enchanted Life Ltd | Education | 2025 |
| Enchanting Retreats Ltd | Real estate | 2024 |
| Encke Ltd | Information & communication | 2022 |
| Encourage 2B Empowered Ltd | Health & social care | 2024 |
| Endcliffe Estates Limited | Hospitality | 2025 |
| Endcroft Management Limited | Information & communication | 2009 |
| Endeavit Limited | Professional & scientific | 2015 |
| Energies Consulting Ltd | Wholesale & retail | 2024 |
| Energy 52 Ltd | Energy | 2026 |
| Energy Network Partners Ltd | Energy | 2024 |
| Enfelista Technologies Ltd | Information & communication | 2024 |
| ENG Deals Ltd | Wholesale & retail | 2023 |
| Engineers Blue Ltd | Professional & scientific | 2025 |
| Engyn I/O Ltd | Professional & scientific | 2025 |
| ENI Properties Ltd | Real estate | 2023 |
| Enigmatic Systems Ltd | Professional & scientific | 2019 |
| Eniwrhand Limited | Wholesale & retail | 2025 |
| Enlightened Pathways Ltd | Information & communication | 2023 |
| Enorence Limited | Professional & scientific | 2024 |
| Enovate Carpentry Ltd | Manufacturing | 2024 |
| Enpact Group Ltd | Information & communication | 2025 |
| Ensa Therapy Ltd | Health & social care | 2025 |
| Ensts Computing Ltd | Wholesale & retail | 2026 |
| ENT Cornwall Limited | Health & social care | 2022 |
| Enterzainment Limited | Information & communication | 2021 |
| Entity Discovery Ltd | Wholesale & retail | 2019 |
| Entour Global Education Ltd | Real estate | 2025 |
| Environmental Research Laboratory Limited | Professional & scientific | 2024 |
| Envirox Ltd | Professional & scientific | 2020 |
| Envision 3D Limited | Manufacturing | 2025 |
| Envision Capital Holdings Ltd | Financial services | 2025 |
| Enzo Apparel Ltd | Wholesale & retail | 2026 |
| Eoes Tech Limited | Information & communication | 2024 |
| EP Events and Entertainment Ltd | Administrative & support | 2024 |
| EP Tech Co Limited | Information & communication | 2026 |
| EPC Renewables Ltd | Construction | 2026 |
| Epic Laser Designs Ltd | Manufacturing | 2021 |
| Epic Projects for Inspiring Communities C.I.C. | Education | 2021 |
| Epicure Community Market Garden CIC | Agriculture & fishing | 2025 |
| Epicurus Business Solutions (UK) Ltd | Professional & scientific | 2009 |
| Epilogue House Limited | Wholesale & retail | 2025 |
| Epiphany Creations Ltd | Information & communication | 2024 |
| Equals Solutions Ltd | Professional & scientific | 2024 |
| Equitable Futures Ltd | Real estate | 2026 |
| Equity Plus Global Trade Ltd | Wholesale & retail | 2025 |
| ER1K Limited | Extraterritorial | 2022 |
| ERC Estates Ltd | Real estate | 2022 |
| ERG Solutions Limited | Health & social care | 2012 |
| Ergolux Group Ltd | Information & communication | 2025 |
| Ergun Global Limited | Wholesale & retail | 2025 |
| Eritque Arcus Limited | Wholesale & retail | 2026 |
| Ernests DEV Ltd | Information & communication | 2025 |
| Errata Studio Ltd | Information & communication | 2026 |
| Ersb Ltd | Wholesale & retail | 2025 |
| Ervins Puksts Studio Limited | Wholesale & retail | 2023 |
| Esbaks Limited | Wholesale & retail | 2023 |
| ESC Media Ltd | Professional & scientific | 2023 |
| Escape Plan Trading Ltd | Wholesale & retail | 2024 |
| Esco Builders Ltd | Construction | 2019 |
| Esensol Ltd | Professional & scientific | 2023 |
| Esgin Limited | Wholesale & retail | 2025 |
| Esha's Light Ltd | Manufacturing | 2024 |
| Eshborn Properties Ltd | Real estate | 2025 |
| Eskander Consulting Ltd | Information & communication | 2022 |
| Essential Studio Ltd | Wholesale & retail | 2025 |
| Essentialia Ltd | Wholesale & retail | 2024 |