| SDS Spedition Ltd | Transport & logistics | 2017 |
| Seasons (Wickersley) Limited | Hospitality | 2018 |
| Select Mot's Limited | Wholesale & retail | 2024 |
| Self Regulatory Body for Sign Language Interpreters and Translators Ltd | Other services | 2015 |
| Serere CIC | Information & communication | 2025 |
| Seven Second Psychology Limited | Professional & scientific | 2025 |
| SEVEN7 Estates Ltd | Real estate | 2024 |
| Sfera Care Ltd. | Health & social care | 2020 |
| SGG Holdings Ltd | Administrative & support | 2017 |
| Shadow Cast Gear Ltd | Manufacturing | 2025 |
| Shaun Bennett UK Ltd | Professional & scientific | 2019 |
| Shedlands Timber Garden Buildings Limited | Manufacturing | 2012 |
| Sheffield Young Professionals Ltd | Other services | 2022 |
| Shirime Branding Ltd | Manufacturing | 2022 |
| Shopify Guro Ltd | Wholesale & retail | 2025 |
| Shosan Ltd | Health & social care | 2026 |
| Silver Birch Facilities Management Limited | Construction | 2005 |
| Simon Peters Construction Limited | Construction | 2002 |
| Simplybd Ltd | Professional & scientific | 2023 |
| SJA Wallace Ltd | Health & social care | 2023 |
| SK3 Properties Limited | Real estate | 2022 |
| Skate Property Portfolio Ltd | Real estate | 2026 |
| Skill Street Trust CIC | Arts & recreation | 2023 |
| Skora Aesthetics Ltd | Health & social care | 2022 |
| Skyguard Flat Roofing Ltd | Construction | 2024 |
| Skymarq Ltd | Transport & logistics | 2026 |
| Slbc Interiors Ltd | Administrative & support | 2024 |
| Sljem Construction Ltd | Administrative & support | 2023 |
| Small Paws Limited | Agriculture & fishing | 2021 |
| SMARTFIX4U Limited | Wholesale & retail | 2025 |
| Smrdp Ltd | Professional & scientific | 2021 |
| SN Cladding Ltd | Construction | 2017 |
| Snap Kabin Co. Limited | Hospitality | 2022 |
| SNB Damp-Proofing Limited | Construction | 2021 |
| SNC Trading Ltd | Transport & logistics | 2025 |
| Sneddon Civils Construction Limited | Professional & scientific | 2022 |
| SO Forward Limited | Transport & logistics | 2022 |
| Soda Scaffolding Limited | Construction | 2012 |
| Sofa District Ltd | Wholesale & retail | 2026 |
| Sofa Studio Ltd | Wholesale & retail | 2018 |
| Sofa Studio Yorkshire Ltd | Wholesale & retail | 2021 |
| Softly Stepping CIC | Health & social care | 2025 |
| Sorrel Ltd | Construction | 2023 |
| Sort Healthcare Ltd | Health & social care | 2016 |
| South Yorkshire Engines Limited | Wholesale & retail | 2024 |
| South Yorkshire Gourmet Catering Limited | Hospitality | 2023 |
| South Yorkshire Maintenance Ltd | Construction | 2025 |
| Southerly Developments Limited | Extraterritorial | 2025 |
| Sowo Property Group Limited | Real estate | 2022 |
| SP Fitness & Training Limited | Arts & recreation | 2025 |
| Sparta Cladding (Northern) Ltd | Administrative & support | 2016 |
| Sparta Cladding Limited | Construction | 2006 |
| Spec Connect Ltd | Construction | 2026 |
| Specialised Wealth Management Limited | Financial services | 2011 |
| Speedfix Cavity Closers Ltd | Manufacturing | 2024 |
| Speedx Logistics Ltd | Transport & logistics | 2025 |
| Spektrum Management Group Ltd | Information & communication | 2020 |
| Spice Lounge (Mexborough) Limited | Hospitality | 2023 |
| Spice Lounge AA Ltd | Hospitality | 2024 |
| Spielers Joinery Limited | Construction | 2015 |
| Sportsbox Swinton Ltd | Hospitality | 2025 |
| Sprocker Specialist Logistics Ltd | Transport & logistics | 2026 |
| Squires Investments Limited | Financial services | 2014 |
| SRI Bars Ltd | Hospitality | 2024 |
| SRK Haulage Ltd | Transport & logistics | 2023 |
| SRK Property Investments Ltd | Real estate | 2022 |
| SRK Rotherham Ltd | Real estate | 2019 |
| SS Brickbuild Developments Limited | Construction | 2025 |
| SS Courier Ltd | Transport & logistics | 2026 |
| Standora Care Limited | Health & social care | 2022 |
| Star Car Wash K Limited | Administrative & support | 2026 |
| Star Fusion Energy Limited | Construction | 2011 |
| Stardust Inns Limited | Hospitality | 2021 |
| Start UP Partner UP Limited | Other services | 2017 |
| Stature Services Group Limited | Construction | 2024 |
| Steel Arch Erection Company Limited | Construction | 2005 |
| Steel City Systems Ltd | Construction | 2026 |
| Stephenson Automation Ltd. | Administrative & support | 2011 |
| Sterispray Ltd | Extraterritorial | 2020 |
| Sterling Knight Holdings Limited | Financial services | 2020 |
| Stevenson Carpets & Flooring Limited | Construction | 2020 |
| STJ Installations Limited | Construction | 2024 |
| Stockley Builds Ltd | Real estate | 2021 |
| Stoker Properties Limited | Manufacturing | 2002 |
| Storai Khan Limited | Wholesale & retail | 2026 |
| Story Industries Ltd | Administrative & support | 2017 |
| Stothards Limited | Other services | 1961 |
| Stott Sharp Ltd | Energy | 2023 |
| Strafford Coatings Limited | Manufacturing | 2021 |
| Stralix Software Technologies Ltd | Information & communication | 2025 |
| Strata Trading Ltd | Administrative & support | 2014 |
| Strategic Enterprise Solutions Ltd | Information & communication | 2005 |
| Stream Connect Limited | Information & communication | 2025 |
| Stripped Parts Limited | Manufacturing | 2014 |
| Strutt & Co Developments Limited | Construction | 2026 |
| Summer Nails Mexborough Ltd | Other services | 2025 |
| Sunnie View Lodge Limited | Real estate | 2022 |
| Sunnyside Supplies Community Interest Company | Hospitality | 2024 |
| Sunshine Care Holdings Limited | Financial services | 2022 |
| Sunshine Care Solutions Limited | Health & social care | 2022 |