| Ec-Ec Fiber Ltd | Construction | 2023 |
| Ecas Group Ltd | Construction | 2025 |
| Ecas Systems Limited | Construction | 2004 |
| Eco Building Compliance UK Ltd | Construction | 2023 |
| ECP Construction Limited | Construction | 2018 |
| ED Services Ltd | Transport & logistics | 2022 |
| EDS of the Valley Motors Ltd | Wholesale & retail | 2025 |
| Edward Convenience Store Ltd | Wholesale & retail | 2023 |
| Edwards Management Solutions Ltd | Information & communication | 2024 |
| EFB Catering Limited | Hospitality | 2024 |
| EFS Systems (Holdings) Limited | Financial services | 2021 |
| EFS Systems (UK) Ltd | Construction | 2012 |
| EG Care Properties Limited | Real estate | 2019 |
| EHS Solutions (UK) Ltd | Administrative & support | 2022 |
| Eight Groundworks Limited | Construction | 2024 |
| Elan Assisted Living Services Ltd | Health & social care | 2010 |
| Elan Dragon Holdings Limited | Financial services | 2022 |
| Electric Motor Repairs Ltd | Manufacturing | 2014 |
| Elite Artists Ltd | Arts & recreation | 2025 |
| Elite Labour Supply Ltd | Administrative & support | 2025 |
| Elite Vinyl Wraps Limited | Construction | 2026 |
| Elliott Sports and Entertainment Ltd | Arts & recreation | 2024 |
| Embarktech Solutions Ltd | Information & communication | 2023 |
| Emerald Fitness Ltd | Other services | 2019 |
| Emerald Training & Consultancy Wales Limited | Professional & scientific | 2005 |
| Empasso Ltd | Wholesale & retail | 2014 |
| Empoweredhw Ltd | Construction | 2019 |
| Empressive Motor Company Limited | Wholesale & retail | 2014 |
| EN Route Travel Merthyr Ltd | Transport & logistics | 2023 |
| Enaid Wellness Ltd | Other services | 2025 |
| Enduraswim Ltd | Education | 2024 |
| Engage at New Pathways Ltd | Wholesale & retail | 2021 |
| Enhanced Football Coaching Ltd | Arts & recreation | 2025 |
| Enhanced Heating and Plumbing Ltd | Construction | 2025 |
| Enterprise Excellence Limited | Professional & scientific | 2014 |
| Enterprize Excellence Ltd | Professional & scientific | 2014 |
| Envision Contractors Ltd | Construction | 2023 |
| EOS Outdoors Ltd | Arts & recreation | 2025 |
| EPT Trans Ltd | Transport & logistics | 2018 |
| Espe Care Limited | Health & social care | 2021 |
| Esportswales CIC | Other services | 2019 |
| Evans and Evans Estates Limited | Real estate | 2022 |
| Evans Carpentry Solutions Limited | Manufacturing | 2021 |
| Evans Fencing Ltd | Administrative & support | 2017 |
| Evans Taxi Ltd | Transport & logistics | 2024 |
| Evolution Safety Solutions Ltd | Professional & scientific | 2022 |
| EWW (Wales) Ltd | Administrative & support | 2008 |
| Excel Hydraulics Ltd | Construction | 2019 |
| Excelsior Electrical Engineering Ltd | Construction | 2024 |
| Executive Travel Wales Ltd | Transport & logistics | 2023 |
| Fairhill Property Advisory Ltd | Real estate | 2026 |
| Family Matters Mediation Ltd | Professional & scientific | 2011 |
| FAR From Sanctuary Escape Ltd | Hospitality | 2026 |
| FAR From Surgical Solutions Ltd | Health & social care | 2019 |
| Fastglass (Wales) Limited | Manufacturing | 2011 |
| Fear & Law Properties Ltd | Construction | 2024 |
| Ffos-Y-Fran (Commoners) Limited | Mining & quarrying | 2003 |
| Ffrind Developments Limited | Construction | 2016 |
| Fine Indian Foods Limited | Hospitality | 2026 |
| Fire Safety Compliance Ltd | Administrative & support | 2020 |
| First Degree Air Conditioning Services Ltd | Construction | 2025 |
| Fit Furniture Ltd | Manufacturing | 2019 |
| Five Star Barber Shop Ltd | Other services | 2025 |
| Fivenineseven Ltd | Professional & scientific | 2013 |
| Fixwell Fasteners Limited | Wholesale & retail | 1990 |
| FKM Pippin Ltd | Construction | 2022 |
| Flec Construction Ltd | Construction | 2026 |
| Flow & Return Ltd | Construction | 2024 |
| Flow Building Services Engineering Ltd | Construction | 2021 |
| Flow Taenellwyr TAN Ltd | Construction | 2017 |
| Fone Bargains Limited | Wholesale & retail | 2025 |
| Fonseca Traffic Management & Rail Solutions Ltd | Construction | 2015 |
| Formation Hair & Beauty Ltd | Other services | 2022 |
| Formova Limited | Construction | 2026 |
| Fountain Fish Bar Ltd | Hospitality | 2022 |
| Fountain Fish Bar Merthyr Ltd | Hospitality | 2019 |
| Fountain Shop Limited | Wholesale & retail | 2004 |
| Four TCS Cwmbran LLP | | 2013 |
| Foxgate Limited | Extraterritorial | 1983 |
| Freezenchill Ltd | Administrative & support | 2022 |
| Fresh Dill Ltd | Wholesale & retail | 2021 |
| Fridays Fish & Chips Limited | Hospitality | 2020 |
| Frontier Power Solutions Ltd | Construction | 2026 |
| Fsvr Associates Ltd | Health & social care | 2026 |
| Full OUT Dance Fitness Ltd | Arts & recreation | 2025 |
| Funky Cabs Limited | Transport & logistics | 2019 |
| Future Digital Education Ltd | Education | 2021 |
| G & M Viazzani Limited | Wholesale & retail | 2003 |
| G Caswell Services Limited | Construction | 2018 |
| G Curtis Rail Limited | Construction | 2017 |
| G Force Telecomms Ltd | Information & communication | 2015 |
| G S James Safety Services Limited | Professional & scientific | 2016 |
| G&B Murphy Rail Limited | Construction | 2022 |
| G&D Thomas Holdings Limited | Wholesale & retail | 2025 |
| G&I Confectionery Limited | Wholesale & retail | 2019 |
| G&M Electrics Ltd | Manufacturing | 2020 |
| G&N Tree and Forestry Services Limited | Agriculture & fishing | 2019 |
| G.P. Electrical & Sons Limited | Manufacturing | 2015 |
| Gaijin Guitars Ltd | Wholesale & retail | 2025 |
| Gainsborough Decorations Limited | Water & waste | 1997 |