| Direct Rendering Limited | Construction | 2018 |
| Distortion Ltd | Information & communication | 2001 |
| DJA Consultancy Ltd | Administrative & support | 2017 |
| DJG Quality Homes Ltd | Real estate | 2018 |
| DJR Consulting Limited | Education | 2026 |
| DM Food Engineering Ltd | Professional & scientific | 2024 |
| Dormouse Heating Services Ltd | Construction | 2011 |
| Douglas E H Hartley Limited | Health & social care | 2011 |
| Dove Cottage Day Hospice | Health & social care | 1996 |
| Dovecote Mill Ltd | Construction | 2024 |
| DPL Prestige Limited | Transport & logistics | 2025 |
| DR Jarvis Psychology Services Ltd | Health & social care | 2023 |
| Driven Automotive Sales Ltd | Wholesale & retail | 2025 |
| Drone Detection Limited | Administrative & support | 2017 |
| DRS Properties Nottingham Ltd | Real estate | 2021 |
| DTI Engineering Ltd | Professional & scientific | 2024 |
| Dual Power Products Limited | Professional & scientific | 1995 |
| Dual Pumps Limited | Manufacturing | 1975 |
| Dualways Limited | Wholesale & retail | 2016 |
| Duffin Builders Limited | Construction | 2006 |
| Duffin Group Ltd | Financial services | 2022 |
| Duffin Homes Ltd | Construction | 2022 |
| Duffin Investments Limited | Real estate | 2015 |
| Duncan Transport Services Limited | Transport & logistics | 2025 |
| Dunstable Road (Luton) Management Co Ltd | Households | 2004 |
| DVS Commercials Ltd | Wholesale & retail | 2015 |
| DVS Holdings & Investments Ltd | Professional & scientific | 2021 |
| Dynamic Brand Solutions Limited | Professional & scientific | 2016 |
| Dynamic Merchandise Limited | Professional & scientific | 2003 |
| Dynapac UK Limited | Construction | 2017 |
| E E Grain Limited | Wholesale & retail | 2016 |
| E Groom Holdings Limited | Financial services | 2026 |
| E Vehicle Charging Limited | Energy | 2021 |
| E&S Building Holdings Limited | Construction | 2026 |
| E-Overcome Limited | Wholesale & retail | 2025 |
| E-Traks Limited | Wholesale & retail | 2012 |
| E.H. Hutton (Coachbuilders) Limited | Manufacturing | 1963 |
| Eagle Crest (Derbyshire) Limited | Hospitality | 2025 |
| Early Years Nursery Limited | Education | 2001 |
| East Coast Metering Services Ltd | Administrative & support | 2022 |
| East Midlands Manufacturing (Leicester) Ltd | Professional & scientific | 2024 |
| East Midlands Manufacturing Advisory Service Limited | Professional & scientific | 2017 |
| East Midlands Padel Limited | Arts & recreation | 2025 |
| Easy Housing Limited | Real estate | 2013 |
| Easy MTD Ltd | Professional & scientific | 2025 |
| Easystart Limited | Wholesale & retail | 1996 |
| EB Creations Limited | Administrative & support | 2018 |
| Ebony Rose Aesthetics Ltd | Other services | 2025 |
| Eclipse Digital Solutions Limited | Professional & scientific | 2001 |
| Eclipse Property Management Limited | Real estate | 2022 |
| Eco Sourcing Group Ltd | Wholesale & retail | 2024 |
| Eco-Friendly Innovations Limited | Wholesale & retail | 2015 |
| Ecosynergy Holdings Limited | Water & waste | 2017 |
| Ecotilities Limited | Administrative & support | 2020 |
| Ecovisum Ltd | Information & communication | 2016 |
| EDA Computer Repairs Ltd | Wholesale & retail | 2023 |
| Edall Ltd | Information & communication | 2023 |
| Edelweizz Ltd | Wholesale & retail | 2015 |
| Edgehill Investments Limited | Real estate | 2018 |
| Edpan Group Limited | Transport & logistics | 2024 |
| Educate Training Limited | Education | 2011 |
| Education Performance Improvement Limited | Information & communication | 2015 |
| Educationcity Limited | Information & communication | 1997 |
| EE Bulk Logistics Ltd | Transport & logistics | 2023 |
| Effective Power Limited | Energy | 2021 |
| Effekt Ltd | Administrative & support | 2017 |
| EKJ Projects Ltd | Administrative & support | 2018 |
| EL Charles Limited | Construction | 2022 |
| Eldon Business Park Limited | Real estate | 2006 |
| Electricalhybrid Ltd | Wholesale & retail | 2022 |
| Electro - Mechanifix Ltd | Manufacturing | 2009 |
| Elegant Wealth Management Limited | Financial services | 2013 |
| Elements of Financial Decisions Limited | Professional & scientific | 2001 |
| Elite Construction and Renovation Ltd | Construction | 2025 |
| Elkington Property Services Ltd | Real estate | 2017 |
| Elliegance Ltd | Other services | 2026 |
| Elliott Composites Ltd | Manufacturing | 2016 |
| Elliott Hair Design Limited | Other services | 2012 |
| Elmere Contracting Ltd | Manufacturing | 2003 |
| Eloise Estates Limited | Real estate | 2021 |
| Emei UK Limited | Financial services | 2021 |
| Emerald Tanning Ltd | Other services | 2025 |
| EMF Property Ltd | Real estate | 2017 |
| Emma Scarborough Garden Design Ltd | Professional & scientific | 2016 |
| Emma Scarborough Garden Management Ltd | Administrative & support | 2016 |
| Empowerment With Laura Ltd | Professional & scientific | 2023 |
| Empwr Sports Limited | Education | 2023 |
| Enderbury's Outfitters Ltd | Wholesale & retail | 2023 |
| Endorphinsport Ltd | Arts & recreation | 2022 |
| Endrate Limited | Wholesale & retail | 2000 |
| ENE Property Management Ltd | Real estate | 2026 |
| Energiframe Limited | Manufacturing | 2010 |
| English Indoor Bowling Association Limited | Arts & recreation | 2008 |
| ENP Property Management Ltd | Real estate | 2021 |
| Ensign Hydraulics Limited | Administrative & support | 2012 |
| Enterprise House (Hartlepool) Management Company Limited | Real estate | 1987 |
| Enterpriz Solutions Limited | Wholesale & retail | 2013 |
| EOS Care Ltd | Health & social care | 2026 |
| Epciengineering.Co.Uk Ltd | Manufacturing | 2018 |
| EPM Construction Consultants Ltd | Extraterritorial | 2016 |