| T Mcleish & Sons Ltd | Construction | 2025 |
| Terrier Plant Hire Ltd | Administrative & support | 2024 |
| THE Borders Crafters Limited | Wholesale & retail | 1998 |
| THE Country Kitchen Deli Limited | Wholesale & retail | 2012 |
| THE Face LAB Melrose Ltd | Other services | 2025 |
| THE Hawick Tartan Company Limited | Wholesale & retail | 2017 |
| THE Learning Nest Ltd | Education | 2024 |
| THE Linthill Garden Partnership | | 1988 |
| THE Medart Aesthetica by DR. Coates Limited | Health & social care | 2024 |
| THE Merlin Academy of Traditional Music Ltd. | Arts & recreation | 2012 |
| THE Pink Poppy Company Limited | Manufacturing | 2024 |
| THE Trading Vault Ltd | Information & communication | 2025 |
| THE Tweed Foundation | Education | 2009 |
| THE Wheezy Frog Ltd | Hospitality | 2025 |
| THE Wild Outdoors Education Company Limited | Education | 2018 |
| THE Wild Outdoors Ltd | Education | 2026 |
| Thehyreco Ltd | Transport & logistics | 2021 |
| Thistle Treks Ltd | Administrative & support | 2019 |
| Thomas Brown & Sons Ltd | Other services | 2012 |
| Thompsons Motors Holdings Limited | Wholesale & retail | 2022 |
| Thompsons Motors Limited | Wholesale & retail | 2014 |
| Thorncroft Services Limited | Professional & scientific | 2025 |
| Thorntrees Amenity Limited | Wholesale & retail | 2002 |
| Three Hills Graphics Limited | Professional & scientific | 2020 |
| Trak MET UK Ltd | Wholesale & retail | 2025 |
| Transition Joinery Ltd | Construction | 2024 |
| Tricapital Angels Limited | Professional & scientific | 2023 |
| Trouver Ltd | Administrative & support | 2017 |
| Tweed Forum Limited | Agriculture & fishing | 1998 |
| Tweed Horizons Ltd | Real estate | 2021 |
| Tweed House Developments Limited | Professional & scientific | 2019 |
| Tweed Private Healthcare Ltd | Financial services | 2026 |
| Unique Cottages Limited | Administrative & support | 2002 |
| Unique Scotland Limited | Administrative & support | 1995 |
| UTS 2 Limited | Construction | 2013 |
| Vafammocc Ltd | Hospitality | 2023 |
| Viewbank Software Limited | Information & communication | 1980 |
| Viewforth Consulting Limited | Administrative & support | 2011 |
| Virtual Channel Limited | Information & communication | 2009 |
| Walkerglobal Psychology Consultants Ltd | Professional & scientific | 2008 |
| Wassmuth-Gibbs Limited | Health & social care | 2012 |
| WE ARE FÀS Ltd | Wholesale & retail | 2025 |
| Welkin Books Limited | Information & communication | 2012 |
| Wellbeing Events Limited | Health & social care | 2019 |
| Westronic (Scotland) Limited | | 1995 |
| White ATV Ltd | Wholesale & retail | 2025 |
| White Dove Studio Ltd | Arts & recreation | 2022 |
| Wild Knights Jousting Ltd | Arts & recreation | 2026 |
| Wilson's of Melrose Ltd | Other services | 2018 |
| Wilton Mills Limited | Construction | 2013 |
| Wood Wealth Ltd | Financial services | 2023 |
| Wooplaw Community Woodlands | Arts & recreation | 2000 |
| Wordbox Language and Publishing Services Ltd | Information & communication | 2010 |
| XR Fabrication Ltd | Manufacturing | 2023 |
| Youthborders | Education | 2006 |
| ZA Management Consultancy Limited | Professional & scientific | 2015 |