| 1880 Catering & Hospitality Limited | Hospitality | 2023 |
| A B Caldwell Ltd | Manufacturing | 2011 |
| A. Dewar Rattray Limited | Wholesale & retail | 1939 |
| A1 Ecosse of AYR Ltd | Transport & logistics | 2020 |
| ABS Glass & Glazing Limited | Construction | 1992 |
| Acneez Ltd | Other services | 2025 |
| Allround GAS Services (Ayrshire) Limited | Construction | 2019 |
| Auntie Pats Boarding Kennels Ltd | Hospitality | 2009 |
| Avartic SCT Ltd | Wholesale & retail | 2024 |
| AYR Coastal Media Ltd | Information & communication | 2023 |
| Ayrshire Chimney Services Ltd | Other services | 2013 |
| Ayrshire Healing Centre Ltd | Health & social care | 2020 |
| B Mcblain Fencing Ltd | Construction | 2023 |
| B-Secure Scotland Ltd | Administrative & support | 2019 |
| BAL Projects Limited | Professional & scientific | 2022 |
| Bankfield Timber Systems Ltd | Construction | 2025 |
| Barra Shell Limited | Real estate | 2005 |
| BDC Fuels Limited | Wholesale & retail | 2002 |
| Beyond Blue Dog Limited | Professional & scientific | 2020 |
| Blair Farm | | 1996 |
| Blawearie Tyres & Tracks Ltd | Wholesale & retail | 2019 |
| Brae Homes (Scotland) Limited | Construction | 1993 |
| Brennan Rail Systems Limited | Professional & scientific | 2026 |
| Briggs Farming Ltd | Agriculture & fishing | 2025 |
| Brockloch Holdings Ltd | Financial services | 2025 |
| Bscit Ltd | Information & communication | 2023 |
| Buildayr Brickwork Ltd | Construction | 2026 |
| C & D Livestock Ltd | Agriculture & fishing | 2024 |
| C Cubed Catering Ltd | Hospitality | 2024 |
| Cameron R Mcculloch Limited | Professional & scientific | 2008 |
| Campbellstyres Ltd | Wholesale & retail | 2024 |
| Capdev Investments Ltd | Financial services | 2026 |
| Caper House Limited | Real estate | 1997 |
| Carlton House Portpatrick Limited | Hospitality | 2017 |
| Carrick Drive 1958 Limited | Extraterritorial | 2014 |
| Carrick Equestrian & Country Limited | Wholesale & retail | 2025 |
| CHM Services Scotland Ltd | Transport & logistics | 2018 |
| Citi Cars Ltd | Transport & logistics | 2025 |
| Cockpen Consultancy Ltd | Professional & scientific | 2024 |
| Community Wellbeing Hub CIC | Other services | 2021 |
| CPM Digital Systems Ltd | Information & communication | 2026 |
| Crawford Henderson Limited | Manufacturing | 1971 |
| Crorieshill Barns Ltd | Hospitality | 2023 |
| Crown Prizes (UK) Ltd | Professional & scientific | 2026 |
| Cube Webworks Limited | Information & communication | 2014 |
| Culzean Food Ltd | Wholesale & retail | 2022 |
| Culzean Fresh Ltd | Hospitality | 2025 |
| D F Hamilton Timber Contractor Ltd | Agriculture & fishing | 2010 |
| D&D Construction Business Ltd | Construction | 2025 |
| D.J. Dunabie (Caravans) Limited | Hospitality | 1971 |
| Dalduff Farm Food & Events Ltd | Hospitality | 2020 |
| David J. Clark Limited | Professional & scientific | 1921 |
| DN International Transport Services Ltd | Transport & logistics | 2025 |
| Dollhouse Beauty Ltd | Other services | 2023 |
| DTS Electrical (Scotland) Ltd | Construction | 2024 |
| Dunroamin' Ayrshire Limited | Professional & scientific | 2015 |
| Dynamic Energy Management Systems Limited | Other services | 2021 |
| Dyrock Developments Limited | Construction | 2015 |
| EAS MOR H2O Ecology Limited | Energy | 2015 |
| EMX Investments Limited | Real estate | 2017 |
| Evolution Designs Ltd. | Information & communication | 2006 |
| F & R Watson Accountants Limited | Professional & scientific | 2017 |
| Falls Plant Ltd | Construction | 2022 |
| Farsight Greentec Ltd | Professional & scientific | 2007 |
| Fenton & Smellie Limited | Financial services | 1955 |
| Fergusson Leisure Limited | Hospitality | 1997 |
| Fish on Scotland Ltd | Agriculture & fishing | 2025 |
| Flyford Holdings Limited | Financial services | 2024 |
| Forever Printworks Ltd | Wholesale & retail | 2020 |
| Fresh Fuel UK Ltd | Manufacturing | 2022 |
| Fusion Contracts Ltd | Manufacturing | 2022 |
| G Malone Services Limited | Construction | 2018 |
| Ganten Scotland Limited | Hospitality | 2012 |
| Good Vibes Gifts Ltd | Wholesale & retail | 2023 |
| Haston Engineering Services Ltd. | Professional & scientific | 1998 |
| Hendrie & Kerr Limited | Energy | 2013 |
| Hollandtech Ltd | Information & communication | 2015 |
| Houndford Holdings Limited | Financial services | 2024 |
| Huntford Holdings Limited | Financial services | 2024 |
| Iain Kirkpatrick Associates Limited | Professional & scientific | 2011 |
| Infinity Gardening and Guttering Solutions Ltd | Administrative & support | 2025 |
| International Packaging Corporation (U.K.) Limited | Manufacturing | 1973 |
| James T Caldwell & Sons LP | | 1989 |
| Jarnet Limited | Information & communication | 2019 |
| JHB Contracts Ltd | Water & waste | 2024 |
| John Lynch (Builders) Limited | Construction | 1976 |
| John Lynch (Developments) Limited | Real estate | 1978 |
| John Lynch Builders AYR Limited | Extraterritorial | 2010 |
| John M. Paterson Ltd. | Wholesale & retail | 2007 |
| Johnstone and Kennedy Ltd | Construction | 2017 |
| K & N Utilities Ltd | Other services | 2020 |
| K M Services Limited | Professional & scientific | 2018 |
| Kirbie Engineering Solutions Limited | Wholesale & retail | 2016 |
| Knoweside Farm | | 2000 |
| Knoxx Transport Ltd | Transport & logistics | 2024 |
| KW Cleaning Services Ltd | Administrative & support | 2023 |
| L-Textiles Limited | Wholesale & retail | 2026 |
| Laidlaw Associates Building Surveying Ltd | Professional & scientific | 2017 |
| Lawrie Design Ltd | Construction | 2019 |
| Linda Dempsey Ltd | Health & social care | 2024 |