| Duckworth Holdings Limited | Financial services | 2021 |
| DUE Auto Motive Ltd | Wholesale & retail | 2017 |
| Durham Close Management Company Limited | Real estate | 2023 |
| DVS Solutions Limited | Wholesale & retail | 2008 |
| DWJ Motors Ltd | Wholesale & retail | 2026 |
| Dyne Group Ltd | Administrative & support | 2022 |
| Dyslexia Digs Ltd | Education | 2021 |
| E Daly Fitness Ltd | Arts & recreation | 2018 |
| E.H.Thorne(Beehives)Limited | Manufacturing | 1960 |
| E2 Design Architecture & Visuals Limited | Professional & scientific | 2026 |
| Eagle Claims Limited | Other services | 2011 |
| Eastfield Associates Lincoln Ltd | Professional & scientific | 2003 |
| Eastgate Lincoln Developments Ltd | Construction | 2024 |
| Eaton Court (Grimsby) Limited | Households | 2024 |
| Ebbey National Conservatories & Windows Limited | Wholesale & retail | 2003 |
| Echelon Command Information Centre Ltd | Administrative & support | 2021 |
| Ecoplant Hire Services Limited | Administrative & support | 2007 |
| Ectotherm Ltd | Construction | 2020 |
| Eden Homes Design Ltd | Construction | 2021 |
| EED & SON Limited | Wholesale & retail | 2001 |
| EJB Electrical Services Ltd | Construction | 2024 |
| ELB Safety Consultancy Ltd | Professional & scientific | 2024 |
| Eleanor Rose Glass Ltd | Wholesale & retail | 2022 |
| Elioteque Ltd | Hospitality | 2020 |
| Elite K9 UK Ltd | Other services | 2018 |
| Elite Panelling Systems Ltd | Construction | 2020 |
| Elland Way (Grainthorpe) Management Company Limited | Households | 2023 |
| Elliott Accountancy Services Limited | Professional & scientific | 2014 |
| Elsham Wold Estates Limited | Real estate | 2007 |
| Elwicks Technical Ltd | Professional & scientific | 2015 |
| Emma’S Paddocks Limited | Agriculture & fishing | 2025 |
| Enchanted Earth Ltd | Wholesale & retail | 2013 |
| Enee Metal Works Ltd | Manufacturing | 2024 |
| Engagerm Limited | Information & communication | 2021 |
| Equidgel Ltd | Manufacturing | 2025 |
| Equidiet (UK) Ltd | Agriculture & fishing | 2014 |
| Equip Global Supplies Ltd | Wholesale & retail | 2002 |
| Equium Wealth Management Limited | Financial services | 2017 |
| Espressivo Chorus Ltd | Arts & recreation | 2016 |
| Essex Chamber Orchestra Limited | Arts & recreation | 2023 |
| Events Itanna Ltd | Hospitality | 2021 |
| Evoko Limited | Professional & scientific | 2021 |
| Evolutionary Homes Ltd | Real estate | 2020 |
| Evolutionary Safety Ltd | Other services | 2024 |
| Executive Response Limited | Financial services | 2001 |
| F E Daubney and SON Limited | Agriculture & fishing | 2012 |
| F&M Cleaning Services Limited | Transport & logistics | 2021 |
| F1 Systems Limited | Construction | 2015 |
| Faldingworth Defence Ltd | Real estate | 2007 |
| Farhana Spice Ltd | Hospitality | 2018 |
| Farm House Developments Ltd | Construction | 2021 |
| FAT Daddy's Smoke Shack Limited | Hospitality | 2023 |
| Fear Properties Ltd | Construction | 2026 |
| Feasible Engineering Ltd | Information & communication | 2026 |
| Fenland Elevator Services Limited | Manufacturing | 2021 |
| Ferrantay Ltd | Professional & scientific | 2024 |
| Festihauls Ltd | Wholesale & retail | 2024 |
| FH Plant Limited | Construction | 2024 |
| FHJ Holdings Ltd | Real estate | 2022 |
| Field Technique Ltd | Agriculture & fishing | 2018 |
| Fieldview Trailers Limited | Manufacturing | 2020 |
| FIG and LIL Ltd | Professional & scientific | 2016 |
| Finance Volt Ltd | Other services | 2021 |
| Financial Consulting Solutions Ltd | Financial services | 2019 |
| Finopsis Ltd | Information & communication | 2018 |
| Fiona Yivli Limited | Health & social care | 2017 |
| Fish Outdoors Ltd | Administrative & support | 2026 |
| Fish Pond Farm Cottages Ltd | Hospitality | 2026 |
| Fjde Properties Ltd | Real estate | 2020 |
| Flames of Newark Limited | Wholesale & retail | 2013 |
| Flaxwell Farming Ltd | Agriculture & fishing | 2025 |
| Florel & Hardy Limited | Extraterritorial | 2015 |
| FLX Prop Co Ltd | Real estate | 2023 |
| Flynn Consultancy Limited | Information & communication | 2008 |
| Focus 7 Limited | Information & communication | 1999 |
| Folly View (Tealby) Management Company Limited | Households | 2018 |
| Food and Drink Entrepreneur of the Year Limited | Administrative & support | 2011 |
| Food Haven Curry and Kebab Ltd | Hospitality | 2026 |
| Food Photographer of the Year Awards Limited | Administrative & support | 2011 |
| Forge Business Park Limited | Real estate | 2025 |
| Forward Business Solutions Ltd | Information & communication | 2025 |
| FOX (Owmby) Limited | Construction | 1964 |
| FOX Aggregates Limited | Transport & logistics | 2010 |
| FOX Earthworks Limited | Mining & quarrying | 2024 |
| FOX Land Holdings Limited | Real estate | 2024 |
| FOX Plant (Owmby) Limited | Professional & scientific | 2007 |
| Friends of Griff Wood Community Interest Company | Public administration | 2015 |
| Frigowny Ltd | Agriculture & fishing | 2025 |
| Front ROW Fencing Ltd | Agriculture & fishing | 2012 |
| Fuel Tank Solutions Limited | Construction | 2019 |
| Fulcrum Homes Ltd | Construction | 2025 |
| Full Thrust Limited | Agriculture & fishing | 2020 |
| Future Solar Care Limited | Extraterritorial | 2023 |
| Fuzzy Solutions Ltd | Manufacturing | 2012 |
| G Howsam & SON Limited | Wholesale & retail | 2013 |
| G&M Safety Consultants Ltd | Professional & scientific | 2007 |
| G.B. Bromley & Sons Limited | Professional & scientific | 1948 |
| G.B. Directional Drilling Services Limited | Construction | 2000 |
| G4 Group Limited | Construction | 2005 |
| Gallamore Units Limited | Construction | 2024 |