| 109 Training Ltd | Education | 2022 |
| 12TH MAN Agency Ltd | Extraterritorial | 2020 |
| 13 Melrose Place Bristol Management Limited | Households | 1989 |
| 1ST for Pets Limited | Administrative & support | 2019 |
| 2 Broomfield Terrace (Whitby) Management Company Limited | Hospitality | 2013 |
| 2 Nicks Limited | Construction | 2007 |
| 23 Incorporated Ltd | Real estate | 2023 |
| 3 Big Dogs Limited | Wholesale & retail | 2017 |
| 43 West Avenue (Filey) Ltd | Real estate | 2017 |
| 56 Birnam Road Freehold Limited | Households | 2020 |
| 7 Acres (York) Ltd | Agriculture & fishing | 2023 |
| 7STREET Limited | Manufacturing | 2009 |
| A & C Thornton Limited | Agriculture & fishing | 2024 |
| A & CH Properties Limited | Real estate | 2020 |
| A & J Plant Sales Ltd | Wholesale & retail | 2023 |
| A C Dental Laboratories Limited | Health & social care | 2008 |
| A Hobson Ltd | Professional & scientific | 2023 |
| A Ross Limited | Arts & recreation | 2002 |
| A&A Roofing Ltd | Construction | 2021 |
| A.C.J.R. Limited | Professional & scientific | 2017 |
| AC Commercials (Malton) Limited | Manufacturing | 1999 |
| ACA Property Maintenance Ltd | Households | 2023 |
| Accessories by Park Lane Limited | Wholesale & retail | 2014 |
| Account Solutions (York) Ltd | Professional & scientific | 2010 |
| Account Solutions Yorkshire Ltd | Professional & scientific | 2019 |
| Acodered Limited | Transport & logistics | 2011 |
| Acorn Community Care | Education | 2002 |
| Adrian Baker Limited | Construction | 2026 |
| Aduco Consultancy Services GB Limited | Professional & scientific | 2019 |
| Advanced Spray Finishing Ltd | Construction | 2022 |
| AFI Management Limited | Real estate | 2003 |
| Afjd Property Ltd | Real estate | 2025 |
| AHH Studio Collective CIC | Arts & recreation | 2019 |
| Airmed Assistance Limited | Transport & logistics | 2020 |
| AJW (Yorkshire) Limited | Construction | 2006 |
| ALA Warranty LLP | | 2013 |
| Alaib Limited | Financial services | 2009 |
| Alec Hare Management Co Ltd | Households | 2016 |
| Alex Lange Ltd | Agriculture & fishing | 2012 |
| Alexander Lauren Limited | Professional & scientific | 2009 |
| Allan Engineering Limited | Professional & scientific | 1992 |
| Alliance Glass (York) Ltd | Construction | 2003 |
| Allison Wold Power Limited | Energy | 2013 |
| Alma Northern Limited | Hospitality | 2025 |
| Alpha Omega Web Solutions Ltd | Information & communication | 2020 |
| AM & KJ Johnston Ltd | Real estate | 2022 |
| AM Refrigeration (Commercial) Ltd | Manufacturing | 2006 |
| Amor Consultancy Limited | Construction | 2017 |
| Amotherby Business Centre Limited | Real estate | 2025 |
| Andesmar Quality Services Limited | Professional & scientific | 2008 |
| Andrei ARY Ltd | Transport & logistics | 2025 |
| Andrew Design & Integration UK Limited | Information & communication | 2005 |
| Andrew Turner Joinery Ltd | Manufacturing | 2021 |
| Andrew Wireless Systems UK Limited | Wholesale & retail | 1988 |
| Animals in Media CIC | Professional & scientific | 2023 |
| Anjb Services Limited | Information & communication | 2016 |
| Anna M T Ltd | Health & social care | 2016 |
| AP Colgan Roofing Ltd | Construction | 2019 |
| Appeal Mobile Development Ltd | Information & communication | 2012 |
| Arcline Architects Limited | Professional & scientific | 2023 |
| Ardea Properties Limited | Real estate | 2025 |
| Arezzo Associates Ltd | Financial services | 2025 |
| Armco IT Consulting Limited | Information & communication | 2004 |
| Arrow Property Maintenance Ltd | Households | 2013 |
| Artisantraditions Wholesale Bakery Ltd | Manufacturing | 2026 |
| Artist Charter Limited | Extraterritorial | 2018 |
| Arts Creative Limited | Arts & recreation | 2024 |
| ASC Performance Ltd | Arts & recreation | 2017 |
| ASC Performance Online Ltd | Professional & scientific | 2023 |
| Ashley Cooke's Carpets and Flooring Malton Limited | Wholesale & retail | 2024 |
| Ashley Cooke's Carpets and Flooring Scarborough Limited | Wholesale & retail | 2024 |
| Assp Group Limited | Financial services | 2024 |
| Assura Consulting Ltd | Professional & scientific | 2025 |
| Aston Hope Enterprises Ltd | Administrative & support | 2022 |
| Athena Joinery & Renovations Limited | Construction | 2021 |
| Audrie Woodhouse Honestly Speaking Limited | Other services | 2024 |
| Augeo Associates Ltd | Financial services | 2026 |
| Automating Automotive Ltd | Professional & scientific | 2019 |
| Avalon Dogs Limited | Agriculture & fishing | 2022 |
| Avenue & Co (York) Limited | Other services | 2022 |
| AWP Wind 1 Ltd | Energy | 2020 |
| AWP Wind 2 Limited | Energy | 2021 |
| AWP Wind 3 Limited | Energy | 2021 |
| B & F Potatoes Limited | Wholesale & retail | 1993 |
| B D Pinkney & Co Ltd | Construction | 2017 |
| B Holdings (York) Ltd | Hospitality | 2023 |
| B M Boothman Agronomy Limited | Agriculture & fishing | 2024 |
| B Magson Precision Ltd | Professional & scientific | 2024 |
| B.E.B Rotating Services Limited | Professional & scientific | 2026 |
| B.M.J. Homes Co. Ltd | Real estate | 2006 |
| Baker Mcveigh and Abbott Limited | Professional & scientific | 2014 |
| Bantam at the OWL Ltd | Hospitality | 2023 |
| Barall Famalam Limited | Real estate | 2018 |
| Barall Holdings Limited | Financial services | 2018 |
| Barall Limited | Real estate | 2018 |
| Battlefield Stud Limited | Agriculture & fishing | 2014 |
| Baviaan Holdings Limited | Financial services | 2025 |
| Bayes Tree Services Ltd | Agriculture & fishing | 2023 |
| BE Amazing Academies Limited | Arts & recreation | 2013 |
| BE Amazing Arts Limited | Arts & recreation | 2015 |