| Durofloor Limited | Construction | 2016 |
| Durtnell (Holdings) Limited | Real estate | 2002 |
| Durtnell Limited | Real estate | 1937 |
| Dustys Cleaning Ltd | Administrative & support | 2017 |
| Dustys Domestic Cleaning Limited | Administrative & support | 2019 |
| Dutchess Limited | Administrative & support | 1988 |
| DUX Shipping Ltd | Wholesale & retail | 2016 |
| Dvape Ltd | Wholesale & retail | 2015 |
| DVB Kent Ltd | Wholesale & retail | 2022 |
| DVM Transport Ltd | Transport & logistics | 2025 |
| DVYH196 Ltd | Financial services | 2019 |
| DWS Services (Southeast) Ltd | Manufacturing | 2014 |
| Dwwhittaker Limited | Professional & scientific | 2014 |
| DXD Design Limited | Other services | 2022 |
| Dxex Ltd | Wholesale & retail | 2025 |
| DY Pro Audio Limited | Wholesale & retail | 2017 |
| Dymock Estates Ltd | Real estate | 2011 |
| Dynamic Project Alliance Ltd | Professional & scientific | 2022 |
| Dynamor Ltd | Construction | 2021 |
| Dysem Ltd | Real estate | 2022 |
| Dziugas Construction Ltd | Manufacturing | 2023 |
| DZL Property Services Ltd | Construction | 2017 |
| E & C Homes Limited | Real estate | 2018 |
| E & G Finance Consultants Ltd | Financial services | 2012 |
| E & K Services Ltd | Administrative & support | 2007 |
| E & S Electronic Ltd | Other services | 2021 |
| E and J Estates Ltd | Real estate | 2025 |
| E and M Design Developments Limited | Administrative & support | 2017 |
| E DAD Consulting Limited | Professional & scientific | 2009 |
| E F Solutions (North) Ltd | Financial services | 2017 |
| E G Butcher & Sons Ltd | Agriculture & fishing | 2023 |
| E M Elliot Ltd | Construction | 2018 |
| E Marketing Limited | Administrative & support | 2024 |
| E Nasir Ltd | Health & social care | 2019 |
| E Soft Solutions Ltd | Construction | 2019 |
| E&D Facades Ltd | Construction | 2014 |
| E&F Property Solutions Ltd | Real estate | 2023 |
| E&F Solutions Ltd | Information & communication | 2017 |
| E&M Cooling Limited | Professional & scientific | 2017 |
| E-Aptitude Ltd | Administrative & support | 2019 |
| E-Grad Ltd | Wholesale & retail | 2021 |
| E-Hun LA. Ltd | Wholesale & retail | 2020 |
| E-Ngauge Limited | Information & communication | 1998 |
| E-Ngauge Software Limited | Extraterritorial | 2007 |
| E-Red Ltd | Wholesale & retail | 2020 |
| E-Sports Distribution Ltd | Wholesale & retail | 2024 |
| E.G.J.A Electrical Ltd | Construction | 2025 |
| E.N.A. Dental Limited | Health & social care | 2026 |
| Eacl Ltd | Real estate | 2013 |
| EAF Rental Ltd | Real estate | 2024 |
| Eagle (Maidstone) Limited | Hospitality | 2024 |
| Eagle Market Trading Ltd | Financial services | 2018 |
| Eagle Transport Ltd | Transport & logistics | 2015 |
| Earl Street Employment Consultants Limited | Administrative & support | 1988 |
| Earl Surveying Services Limited | Professional & scientific | 2025 |
| Early Learning Steps Ltd | Education | 2019 |
| Earth and Sun Ltd | Agriculture & fishing | 2022 |
| Earth by Trunk Limited | Wholesale & retail | 2023 |
| Earth Executive Cars Limited | Transport & logistics | 2022 |
| Earth Horse Wisdom Ltd | Information & communication | 2026 |
| Earthling Films Ltd | Information & communication | 2018 |
| Earwax Ladies Limited | Health & social care | 2024 |
| Easilysync Ltd | Information & communication | 2026 |
| East Coast Sporting Ltd. | Arts & recreation | 2014 |
| East Kent Opportunities Limited Liability Partnership | | 2008 |
| East Kent Underwriting Ltd | Financial services | 2001 |
| East of India (Kent) Limited | Extraterritorial | 2025 |
| East Sussex Design and Assembly Limited | Manufacturing | 2019 |
| Eastbrook Systems Ltd | Information & communication | 2005 |
| Eastham Game Exchange Limited | Wholesale & retail | 2010 |
| Easthaus Limited | Real estate | 2016 |
| Easy Access Plant Hire Ltd | Administrative & support | 2021 |
| Easy Financial South West Limited | Financial services | 2014 |
| Easy Money Limited | Professional & scientific | 2006 |
| Easy Ride CAB Ltd | Transport & logistics | 2025 |
| Easydine Limited | Hospitality | 2020 |
| Easyfold Doors Ltd | Manufacturing | 2020 |
| EAT Fresh Catering Ltd | Hospitality | 2026 |
| EAU Solutions Ltd | Professional & scientific | 2023 |
| Ebbsfleet Recycling Company Limited | Construction | 2017 |
| Ebenazer Limited | Health & social care | 2017 |
| Ebiz Properties Ltd | Real estate | 2020 |
| EC Design by Concrete Limited | Construction | 2018 |
| EC Global UN Limited | Financial services | 2026 |
| ECC Holdings (Kent) Ltd | Financial services | 2026 |
| Echno Limited | Wholesale & retail | 2007 |
| Echo Engineering (Southern) Limited | Construction | 1997 |
| Echo Wheels Pulse Ltd | Transport & logistics | 2025 |
| Ecko Resources Ltd | Administrative & support | 2017 |
| ECL Healthcare Limited | Health & social care | 2022 |
| Eclectic Care Ltd | Health & social care | 2021 |
| Eclectic Estates Ltd | Real estate | 2023 |
| Eclipse Connections Ltd | Administrative & support | 2026 |
| Eclipse Global Ltd | Administrative & support | 2019 |
| Eclipse HR Limited | Administrative & support | 2023 |
| Ecliptica Ltd | Transport & logistics | 2024 |
| ECM Contractor Ltd | Construction | 2023 |
| Eco Green Store Ltd | Wholesale & retail | 2025 |
| Eco MEP Contractors Limited | Construction | 2015 |
| Ecola & K Ltd | Construction | 2025 |