| Ensign Park Limited | Professional & scientific | 1989 |
| Enslow Mill Management Company Limited | Households | 2019 |
| Ensollex Ltd | Information & communication | 2016 |
| Enstone Airlines Limited | Manufacturing | 1995 |
| Enstone Aviation Ltd | Transport & logistics | 2020 |
| Enstone Hangar Limited | Transport & logistics | 2018 |
| Enstone Ventures Ltd | Transport & logistics | 2014 |
| Entec Access Systems Limited | Construction | 2006 |
| Enterprise Nation Ltd | Other services | 2003 |
| Entrepreneurship Ltd | Information & communication | 2022 |
| Enviroclad Group Ltd | Construction | 2021 |
| Envirocomm Limited | Professional & scientific | 2007 |
| Envirotemp Limited | Construction | 2012 |
| Envy Hair & Beauty Salon Ltd | Other services | 2009 |
| EON Maidenhead Ltd | Hospitality | 2026 |
| EOS Claims LLP | | 2012 |
| EOS Software Solutions Limited | Information & communication | 2017 |
| EPB Builders Ltd | Construction | 2013 |
| Epic Brownies Ltd | Manufacturing | 2023 |
| Eplum Ltd | Construction | 2025 |
| Epochracy Ltd | Other services | 2023 |
| Epsom Slate & Tile Limited | Construction | 2015 |
| EQUE2 Limited | Information & communication | 2012 |
| Equilink Limited | Professional & scientific | 2011 |
| Equimatrix Ltd | Information & communication | 1991 |
| Equine Dental Services South East Limited | Professional & scientific | 2017 |
| Equine Projects Limited | Agriculture & fishing | 2017 |
| Equinova Limited | Professional & scientific | 2024 |
| Equipe Prosolutions Limited | Professional & scientific | 2013 |
| Equus Imports Limited | Other services | 2007 |
| Erasmusmusic Limited | Information & communication | 2018 |
| Eress Property Ltd | Other services | 2018 |
| Ernest Barnes Limited | Construction | 2009 |
| Eroundit Ltd | Information & communication | 2013 |
| ES Electronics Limited | Information & communication | 2015 |
| ESA International Recruitment Ltd | Administrative & support | 2017 |
| ESCAPE3600 Limited | Arts & recreation | 2007 |
| Eshaan Tech Ltd | Other services | 2018 |
| ESS Electrical Services Limited | Construction | 2021 |
| Essencial Consultants Ltd | Professional & scientific | 2020 |
| Essencial Trade Ltd | Professional & scientific | 2019 |
| Essential First AID Ltd | Health & social care | 2024 |
| Esser Works Racing Limited | Wholesale & retail | 2021 |
| Estate Crafters Ltd | Construction | 2024 |
| Estate Eighty Eight Limited | Real estate | 2022 |
| Este Hair Medical Clinic (Maidenhead) Ltd | Health & social care | 2026 |
| Esteve Pharmaceuticals Ltd | Manufacturing | 1997 |
| Esteve RD UK & Ireland Ltd. | Wholesale & retail | 2009 |
| Estivia Limited | Professional & scientific | 2016 |
| Etanaina Stay Ltd | Hospitality | 2023 |
| Eterno Medicolegal Ltd | Health & social care | 2020 |
| Eterno Plastic Surgery Limited | Health & social care | 2015 |
| Etidesign Ltd | Professional & scientific | 2021 |
| Eton Nest Ltd | Real estate | 2016 |
| Eton Panel Systems Limited | Manufacturing | 1999 |
| Eton Travel Agency Limited | Administrative & support | 1983 |
| Eton Travel Holdings Limited | Administrative & support | 2001 |
| Europaths Football Ltd | Arts & recreation | 2019 |
| European Star Limited | Real estate | 1996 |
| Euroscrapmetal-Uk Ltd | Water & waste | 2023 |
| Eveatec Limited | Professional & scientific | 2014 |
| Event Centric Limited | Extraterritorial | 2010 |
| Event Crew Ltd | Hospitality | 2025 |
| Event Dressing Co Ltd | Administrative & support | 2026 |
| Event Solution Limited | Administrative & support | 2004 |
| Eveolve Ltd | Extraterritorial | 2021 |
| Ever After Edits Ltd | Information & communication | 2025 |
| Ever Blue Ocean Ltd. | Construction | 2016 |
| Everest Acquisition Company Limited | Information & communication | 2017 |
| Evergen Group Ltd | Financial services | 2023 |
| Evergen Power Ltd | Professional & scientific | 2023 |
| Evergen Systems Ltd. | Professional & scientific | 2011 |
| Evergreen Explorers CIC | Education | 2025 |
| Evernicecare Limited | Health & social care | 2022 |
| Evil Media Ltd | Information & communication | 2024 |
| Evolution Organics Limited | Wholesale & retail | 2004 |
| Evolve Audio Visual Limited | Information & communication | 2015 |
| Evolveyou App Limited | Administrative & support | 2017 |
| EWI Render Ltd | Construction | 2023 |
| Example Digital Ltd | Administrative & support | 2018 |
| Exceed Electrical Ltd | Construction | 2020 |
| Excel Marble Ltd | Manufacturing | 2025 |
| Excel New Homes Limited | Construction | 2021 |
| Excel Pumps and Motors Ltd. | Manufacturing | 2001 |
| Excellence Electrical Ltd | Construction | 2008 |
| Excellent Education for Everyone Limited | Education | 2016 |
| Excellent Plants Ltd | Wholesale & retail | 2010 |
| Exclusive Traders Ltd | Wholesale & retail | 2024 |
| Executive Advisory Services Limited | Administrative & support | 1992 |
| Exelux Air Travel Services Limited | Transport & logistics | 2012 |
| Exelux Estates Limited | Real estate | 2021 |
| Eximbills Technologies Ltd | Information & communication | 2001 |
| Exinterp Ltd | Information & communication | 2017 |
| Exlers Garage Ltd | Wholesale & retail | 2008 |
| Exoextraction Ltd | Information & communication | 2024 |
| Exotic Number Plates Limited | Manufacturing | 2020 |
| Exotic Real Estate Limited | Real estate | 2020 |
| EXP32 Ltd | Information & communication | 2022 |
| Expert Conservatories and Double Glazing Limited | Manufacturing | 2025 |
| Expert Employment Limited | Professional & scientific | 2009 |