| 2 Spa Cottages Limited | Hospitality | 2016 |
| A & B Supply Co. Limited | Wholesale & retail | 2013 |
| Accurate Internet Marketing & Design Ltd | Information & communication | 2011 |
| Aqua-Eye Limited | Information & communication | 2013 |
| Arvid Transport Ltd | Manufacturing | 2007 |
| Aston & Co UK Ltd | Professional & scientific | 2011 |
| AT Groundworks Ltd | Construction | 2024 |
| Axis Renewable Energy Ltd | Construction | 2011 |
| Barnstorm Theatre Community Interest Company | Arts & recreation | 2022 |
| Beton Services Ltd | Manufacturing | 2003 |
| Broadoak Group Limited | Construction | 2017 |
| Brooklea Gardens Longhope Management Limited | Households | 2005 |
| Broom Hill Retreat Ltd | Hospitality | 2022 |
| Callidus ROE Limited | Real estate | 2016 |
| Carter and Bond Limited | Wholesale & retail | 2002 |
| Chance & Noble Ltd | Real estate | 2015 |
| Cheltenham Tyre Services Limited | Wholesale & retail | 2010 |
| Chris Compton Construction Ltd | Construction | 2011 |
| Cosine Holdings Ltd | Information & communication | 2019 |
| Cotswold Cosy Cottage Limited | Hospitality | 2014 |
| Daveangelsons Properties Ltd | Real estate | 2022 |
| Dcforza Ltd | Wholesale & retail | 2012 |
| Dean Forest Kitchen CIC | Hospitality | 2022 |
| DJ Sherratt Plumbing & Heating Ltd | Construction | 2021 |
| DJW Building Services Limited | Construction | 2011 |
| DK247 Ltd | Wholesale & retail | 2025 |
| Double M Construction Ltd | Construction | 2020 |
| Double M Development Ltd | Real estate | 2014 |
| Duberley FI Ltd | Real estate | 2025 |
| Dymock Grange Golf Ltd | Arts & recreation | 2025 |
| Eden & Hive Limited | Other services | 2022 |
| Elevate Interior Solutions Ltd | Wholesale & retail | 2021 |
| Elliott Business Consulting Ltd | Professional & scientific | 2025 |
| Enviroderm Services (UK) Limited | Professional & scientific | 2001 |
| Excalibre Manufacturing Ltd | Other services | 2025 |
| Farmers BOY Inn (Longhope) Limited | Hospitality | 2013 |
| Flamin Edges Ltd | Hospitality | 2020 |
| Foran Homes Limited | Real estate | 2021 |
| Forest of Dean Limited | Agriculture & fishing | 2020 |
| Forest of Dean Reclamation and Building Limited | Construction | 2009 |
| Forest Physiotherapy Ltd | Other services | 2013 |
| Gable & ROE Ltd | Real estate | 2026 |
| Giddy Wintle Services Limited | Professional & scientific | 2014 |
| Gryffyn Productions Limited | Information & communication | 2013 |
| H K Developers (Longhope) Limited | Construction | 2020 |
| Hamilton House Estates Limited | Real estate | 1999 |
| Hamilton House Group Limited | Extraterritorial | 1999 |
| Hamilton House International Limited | Extraterritorial | 1999 |
| Hamilton House Leisure Limited | Extraterritorial | 1999 |
| Hannah Court Barrister Ltd | Professional & scientific | 2026 |
| Hannah Court Holdings Ltd | Financial services | 2026 |
| Harts Barn Cookery School Limited | Hospitality | 2023 |
| Herome Ltd | Real estate | 2025 |
| Hillgrove Acquisitions Limited | Real estate | 2021 |
| Hopeswood Ltd | Health & social care | 2012 |
| I & M Golf Ltd | Arts & recreation | 2018 |
| Iamrossmason Limited | Information & communication | 2023 |
| Iecm Consulting Limited | Professional & scientific | 2024 |
| Indent Ltd | Professional & scientific | 2025 |
| Intocleaning Limited | Administrative & support | 2003 |
| Ixian Devices Limited | Information & communication | 1984 |
| J Lear Design Limited | Information & communication | 2019 |
| J. Barnes Groundworks Limited | Construction | 2025 |
| JJ Consulting Solutions Limited | Health & social care | 2013 |
| Kettle Civils Limited | Construction | 2011 |
| Kiernans Property Limited | Real estate | 2015 |
| Longhope Motor Services Ltd | Wholesale & retail | 2014 |
| Losu Ltd | Arts & recreation | 2023 |
| M-Build Construction Limited | Construction | 2021 |
| Maple (482) Limited | Households | 2022 |
| Mark Saunders Architectural & Building Surveying Services Ltd | Professional & scientific | 2025 |
| Matt Stead Ukulele Ltd | Education | 2025 |
| MAY Hill Woodlands Ltd | Agriculture & fishing | 2018 |
| Mcevans Productions Limited | Construction | 2014 |
| Miss Hamilton Limited | Extraterritorial | 2006 |
| MLS Accountancy Services Limited | Professional & scientific | 2015 |
| Mondello Three Ltd | Construction | 2021 |
| MTS Carpentry Contractors Ltd | Construction | 2014 |
| MTS Scaffolding Ltd | Construction | 2017 |
| Nerc Precision Engineering Limited | Manufacturing | 2018 |
| Netstar Limited | Information & communication | 2006 |
| Oakley Property Renovations Ltd | Construction | 2024 |
| Orchard Recycling Service Ltd | Water & waste | 2014 |
| Papaya Homes Ltd | Construction | 2021 |
| Penny Gyngell Ltd | Hospitality | 2014 |
| Permali Deho Limited | Manufacturing | 1969 |
| PF Coaching Ltd | Education | 2020 |
| Phoenix Wood Burning Installations Ltd | Construction | 2024 |
| Pointlease Property Management Limited | Hospitality | 1990 |
| Poole Graham Wealth Consultancy Ltd | Financial services | 2018 |
| Progressive Milk Producers Limited | Agriculture & fishing | 1987 |
| Quality Medical Services Ltd | Manufacturing | 2022 |
| Renegade Media Limited | Professional & scientific | 2002 |
| Reyacaso Ltd | Wholesale & retail | 2023 |
| RJM Construction Ltd | Construction | 2024 |
| Rocketscience Media Limited | Information & communication | 2025 |
| Rogan Tucker Limited | Real estate | 2018 |
| Samurai Scaffolding Ltd | Construction | 2024 |
| Selkirk Media Ltd | Information & communication | 2014 |
| Simple Hygiene Solutions Ltd | Administrative & support | 2013 |