| Rely Health Care Ltd | Health & social care | 2023 |
| Relymed Limited | Health & social care | 2021 |
| RG Investigation Services Ltd | Administrative & support | 2016 |
| RNE Ltd | Construction | 2025 |
| Romeo Persimmon Ltd | Real estate | 2024 |
| Roofworx Roofing & Building Contractors Ltd | Construction | 2025 |
| Roseline Entertainment Limited | Information & communication | 2013 |
| Ross Johnston Electrical Services Limited | Construction | 2016 |
| Rosslyn Distillery Ltd | Manufacturing | 2024 |
| Ruichang Limited | Hospitality | 2024 |
| Rutland Square Spirits Ltd | Manufacturing | 2015 |
| Sacs Management Ltd | Other services | 2014 |
| Safe Simple Secure Limited | Administrative & support | 2008 |
| Saltire Hospitality Limited | Hospitality | 2000 |
| Saltire Investments (Scotland) Ltd | Real estate | 2021 |
| Saltire Patisserie Ltd. | Manufacturing | 2006 |
| Sanal's Limited | Hospitality | 2023 |
| SAS Blacksmiths & Steel Fabricators Ltd | Construction | 2020 |
| Sassan Scot Limited | Wholesale & retail | 2025 |
| Satellite Aerial Trade Supplies Ltd | Manufacturing | 2016 |
| SAW and Tooling Holdings Limited | Financial services | 2024 |
| Sbeaston Renewable Ltd | Professional & scientific | 2017 |
| SCALES2TAILZ Limited | Agriculture & fishing | 2025 |
| Scallywags Children's Nursery Loanhead Limited | Education | 2015 |
| SCOTB123 Ltd | Arts & recreation | 2019 |
| Scotbet Limited | Arts & recreation | 1996 |
| Scotia Adventure Ltd | Education | 2025 |
| Scotia Safes Holdings Limited | Manufacturing | 2024 |
| Scotia Safes Limited | Manufacturing | 1974 |
| Scottish Association for Country Sports | Financial services | 2014 |
| Select MS Limited | Construction | 2025 |
| Sensational Systems Limited | Manufacturing | 2014 |
| Sentra Risk & Protection Ltd | Administrative & support | 2025 |
| Sentu Wellness Ltd | Other services | 2019 |
| Sherbrook Construction Ltd | Construction | 2026 |
| Sherbrook Facilities Management Ltd | Professional & scientific | 2025 |
| Sherbrook Management Services Limited | Professional & scientific | 2013 |
| Sheriff Motors Ltd | Wholesale & retail | 2025 |
| SHL Modelling Ltd | Information & communication | 2007 |
| Sigmaqu Ltd | Information & communication | 2024 |
| Simple Homes Scotland Ltd | Real estate | 2017 |
| Sinclair Scott Limited | Construction | 1986 |
| Sinclair Scott Properties Limited | Real estate | 2000 |
| Skirving Electrical Ltd | Manufacturing | 2017 |
| Skymuir Limited | Transport & logistics | 2000 |
| Sonsie Face Limited | Wholesale & retail | 2016 |
| SOS GIN Ltd | Manufacturing | 2017 |
| Specialist Welding and Pipe Services Ltd | Manufacturing | 2026 |
| Sports Warehouse (Edinburgh) Limited | Wholesale & retail | 1973 |
| Sports Warehouse Ltd | Wholesale & retail | 2015 |
| Spotless Maid Cleaning Ltd | Administrative & support | 2021 |
| SRI Venkata Limited | Hospitality | 2026 |
| ST Margaret's Farm Business Units Ltd | Real estate | 2025 |
| Star Helix Ltd | Manufacturing | 2023 |
| Steading LOG Co Ltd | Wholesale & retail | 2022 |
| Steading Trading Company Ltd | Other services | 2017 |
| Stewart Surveying Services Ltd | Professional & scientific | 2024 |
| Stopfire Limited | Public administration | 2003 |
| Stopfire Offsite Coatings Limited | Construction | 2008 |
| Sugar Pine Brewing Company Limited | Manufacturing | 2002 |
| Superlogo Limited | Wholesale & retail | 2004 |
| Survey Solutions Scotland (Sales) Partnership | | 2025 |
| SY Engineering Ltd | Construction | 2025 |
| T Hill (Plumbing & Heating) Limited | Construction | 2007 |
| Tantallon Press, Limited | Manufacturing | 1959 |
| Tartan Trades Ltd | Construction | 2018 |
| Tasty Loaf and Cake Ltd | Wholesale & retail | 2025 |
| THE Beauty of Edinburgh Ltd | Agriculture & fishing | 2021 |
| THE Book Whisperers Community Interest Company | Information & communication | 2008 |
| THE David Gray Testimonial Limited | Arts & recreation | 2023 |
| THE Dormant Distillery Company Limited | Wholesale & retail | 1997 |
| THE Hive Zone Consultancy Ltd | Professional & scientific | 2024 |
| THE Pentland Still Ltd | Manufacturing | 2017 |
| THE Power Diaspora Forum Limited | Arts & recreation | 2025 |
| THE Prosper Diaspora Forum Limited | Arts & recreation | 2025 |
| THE Salter Centre Trading Ltd | Real estate | 2023 |
| THE Secrets of Caledonia Co. Ltd | Wholesale & retail | 2019 |
| THE Select Academy Ltd | Professional & scientific | 2025 |
| THE Smith Emsley Partnership Limited | Professional & scientific | 2003 |
| THE Source Coffee Co Ltd | Administrative & support | 2019 |
| Theloan Ltd | Construction | 2021 |
| Thermalhub Ltd | Professional & scientific | 2022 |
| Thermoglaze Ltd | Construction | 2023 |
| Thistle Design (M M C) Limited | Manufacturing | 1996 |
| Timbertech (Scotland) Limited | Manufacturing | 2003 |
| Total Project Management Group Ltd | Manufacturing | 2018 |
| Treatz Limited | Wholesale & retail | 2023 |
| Treble 2 Ltd | Education | 2011 |
| TRU' Loaf Home Bakery Ltd | Wholesale & retail | 2018 |
| Tully Roofing Ltd | Construction | 2017 |
| Turing IT Ltd | Other services | 2023 |
| Twig Garden Design Ltd | Administrative & support | 2012 |
| UNI Store Storage Limited | Transport & logistics | 2024 |
| UNI Windows Ltd | Construction | 2011 |
| Universal Cleaning Academy Limited | Administrative & support | 2023 |
| Upnorth Biltong LP | | 2018 |
| UX Consulting Ltd | Information & communication | 2017 |
| Valkyrie Fitness Group Ltd | Arts & recreation | 2026 |
| Vanessa Brodrick Limited | Arts & recreation | 2017 |
| Vapotherm UK Ltd | Wholesale & retail | 2009 |