| A - Comm Ltd | Information & communication | 2001 |
| Aberlleiniog Limited | Hospitality | 2025 |
| Addams Family Estate Limited | Construction | 2024 |
| Afallon Ltd | Professional & scientific | 2013 |
| ALA the VET Limited | Professional & scientific | 2022 |
| Andreou Property Group Ltd | Wholesale & retail | 2022 |
| Anglesey Column Trust | Arts & recreation | 2007 |
| Anglesey Electrical Contractors Ltd | Construction | 2020 |
| Anglesey Heritage Construction Limited | Construction | 2025 |
| Anglesey Maids Ltd | Administrative & support | 2021 |
| Anglesey Menai Cottages Ltd | Administrative & support | 2017 |
| Anglesey Model Village Ltd | Hospitality | 2016 |
| Anglesey Woodland Services Limited | Agriculture & fishing | 2021 |
| Artisan Plants UK Ltd | Agriculture & fishing | 2021 |
| ASG Automation Ltd | Professional & scientific | 2024 |
| Atlas Community Ltd | Information & communication | 2025 |
| Barony 2020 Ltd | Hospitality | 1998 |
| Become ZW Ltd | Wholesale & retail | 2019 |
| Bennett Building Group Limited | Construction | 1969 |
| BIRDM888 Ltd | Hospitality | 2025 |
| Bodlondeb Store Ltd | Wholesale & retail | 2026 |
| Brooke & Associates Consulting Limited | Professional & scientific | 2015 |
| Butler & Butler Consulting Ltd | Professional & scientific | 2011 |
| C&M Groundworks Ltd | Water & waste | 2025 |
| Caernarvonshire & Anglesey Motor Club Limited | Arts & recreation | 1984 |
| Calon MON Limited | Health & social care | 2011 |
| Calon Y Coed Community Hub CIC | Agriculture & fishing | 2025 |
| Camau Nesaf Limited | Professional & scientific | 2015 |
| Carrigan Solutions Limited | Manufacturing | 2026 |
| Castell Lleiniog Holidays Limited | Hospitality | 2024 |
| Ceir PG Taxi Limited | Transport & logistics | 2025 |
| Cerian Griffith CYF | Arts & recreation | 2021 |
| Cerrig Y Barcud Holiday Cottages Limited | Hospitality | 2025 |
| Charlotte Faith Ltd | Professional & scientific | 2025 |
| Chelo Properties Limited | Professional & scientific | 2003 |
| Chris Owens Homes and Construction Ltd. | Construction | 2013 |
| Clogwyn Limited | Agriculture & fishing | 2018 |
| Clwydian Consultants Limited | Professional & scientific | 2002 |
| Clynnog Farm Maintenance Ltd | Households | 2006 |
| Coastal Holidays Ltd | Administrative & support | 2013 |
| Coho Alto Ltd | Manufacturing | 2021 |
| Criw Niwbwrch CYF | Arts & recreation | 2003 |
| Cwmni Sefydledig Prichard Jones CYF | Arts & recreation | 2004 |
| Cyfoeth Jones Wealth Limited | Professional & scientific | 2018 |
| David Ellis Consultancy Limited | Professional & scientific | 2023 |
| David Jones & SON Limited | Construction | 2019 |
| DE Bechevel Limited | Hospitality | 2004 |
| Dewis Dillad CYF. | Professional & scientific | 2025 |
| Diamond Shine Cleaning and Oven Cleaning Limited | Administrative & support | 2015 |
| Ecocyclen Ltd | Construction | 2010 |
| EFG Local Limited | Wholesale & retail | 2023 |
| Eglas Limited | Administrative & support | 2012 |
| Egni MON Renewables Limited | Construction | 2023 |
| Elemental (NW) Limited | Professional & scientific | 2026 |
| Elfin Productions Limited | Information & communication | 2008 |
| Embrella Labs Ltd | Information & communication | 2026 |
| Emhhealthcareconsulting Ltd | Education | 2024 |
| Euros Jones Ltd | Health & social care | 2025 |
| Ewan Kennaway Ltd | Transport & logistics | 2024 |
| Financial Integrity Mark Limited | Professional & scientific | 2026 |
| Furqanali Ltd | Wholesale & retail | 2025 |
| G Williams Projects Limited | Construction | 2020 |
| G-Sports Consulting Ltd | Professional & scientific | 2020 |
| Gbfra Limited | Public administration | 2023 |
| Geal Joinery Limited | Manufacturing | 2003 |
| Gems Laundry Ltd | Administrative & support | 2022 |
| George Property Management Ltd | Real estate | 2021 |
| Go Gutter Ltd | Manufacturing | 2024 |
| Granic 7 Limited | Wholesale & retail | 2000 |
| Grwp AP Fychan CYF | Financial services | 2019 |
| Gwynedd Auto Electrics Ltd | Wholesale & retail | 2026 |
| Gylfinir’S Rock Ltd | Professional & scientific | 2022 |
| Hogan Heating Services Limited | Construction | 2020 |
| HUW Williams Driver Training (Ynys MON) Limited | Education | 2009 |
| Idiomatig CYF | Education | 2018 |
| J & C Local Limited | Wholesale & retail | 2024 |
| JLR Joinery Ltd | Construction | 2024 |
| Jones Llan Ltd | Wholesale & retail | 2023 |
| JTP Mechanical Ltd | Construction | 2020 |
| Kalon Creations Ltd | Hospitality | 2024 |
| Kami Pro Limited | Real estate | 2025 |
| Ladies Working Dog Group Events Ltd | Administrative & support | 2024 |
| Law Limited | Extraterritorial | 2016 |
| LLE 6 Limited | Real estate | 2019 |
| Llwyn ONN Management Company Limited | Households | 2018 |
| Llwynonn Holiday Homes Ltd | Hospitality | 2020 |
| Llyn Parc Mawr Community Woodland Group Ltd | Agriculture & fishing | 2016 |
| Louise Bibby Law Ltd | Professional & scientific | 2025 |
| MDR Estate Limited | Real estate | 2023 |
| Meithrinfa Siwgr Plwm CYF | Administrative & support | 2009 |
| Menai Accounting Ltd | Information & communication | 2021 |
| Menai Scientific Consulting Limited | Professional & scientific | 2026 |
| Merddyn Court Management Limited | Other services | 2011 |
| Merelian Limited | Financial services | 2010 |
| Mermaid Residential Ltd | Real estate | 2026 |
| Metxbio Ltd | Professional & scientific | 2014 |
| Miglaioma Limited | Real estate | 2022 |
| MON Accountancy Limited | Professional & scientific | 2019 |
| Noveltune Ltd | Information & communication | 2021 |
| Oldfield Construction Limited | Agriculture & fishing | 2002 |