| Romano 434 Ltd | Real estate | 2018 |
| Romold Limited | Manufacturing | 2002 |
| Rooftop Properties Ltd. | Real estate | 2003 |
| Ropri Enterprises Ltd | Wholesale & retail | 2026 |
| Rory Andrew Limited | Professional & scientific | 2016 |
| Rosewood Homes and Properties Limited | Construction | 2010 |
| Ross Mckenzie PT Ltd | Arts & recreation | 2023 |
| Rothera Group Limited | Professional & scientific | 2008 |
| Rothnick Ltd | Real estate | 2012 |
| Route Runner Ltd | Professional & scientific | 2025 |
| Rowan Horticultural, Property and Consultancy Services Ltd | Administrative & support | 2020 |
| Royale Rizq Ltd | Wholesale & retail | 2025 |
| RS Decorations and Events Ltd | Hospitality | 2023 |
| RS Unlimited Ltd | Wholesale & retail | 2025 |
| RTS Scot Ltd | Transport & logistics | 2025 |
| Rubicon Archaeology Ltd | Professional & scientific | 2018 |
| Russell Joinery Ltd | Construction | 2021 |
| Rutland Independent Wealth Management LLP | | 2015 |
| S Boyd Ltd | Financial services | 2020 |
| S L Arthur (SLA) Services Limited | Information & communication | 2024 |
| S&A Tuition Ltd | Education | 2021 |
| S&C Edinburgh Limited | Hospitality | 2025 |
| S&Lm Construction Ltd | Administrative & support | 2024 |
| S&S Cleaning Services Ltd | Administrative & support | 2016 |
| S&S Invest Ltd | Real estate | 2025 |
| S&S Uphall Ltd | Wholesale & retail | 2018 |
| S.H.A Instruments Ltd | Manufacturing | 2023 |
| S1 Payments Ltd | Wholesale & retail | 2020 |
| S@Iven Lettings Ltd | Real estate | 2024 |
| SAC Installs Ltd | Construction | 2023 |
| SAE Consulting Services Limited | Information & communication | 2011 |
| Safeguard GAS Engineers Ltd | Construction | 2018 |
| SAI Lakshmi Nilayam Property Ltd | Real estate | 2023 |
| Saiesh Properties Limited | Real estate | 2022 |
| Salt Hair and Aesthetics Ltd | Other services | 2023 |
| Saltirecontracts Limited | Manufacturing | 2025 |
| Samco Interiors Limited | Manufacturing | 2016 |
| Samuel Housing Ltd | Real estate | 2020 |
| Samuel Technologies Ltd | Information & communication | 2011 |
| Saor Guitars Ltd | Manufacturing | 2024 |
| SAR Commercial Limited | Real estate | 2019 |
| SAR IT Consultants Ltd | Information & communication | 2025 |
| Sara Sharp Family Chiropractor Limited | Health & social care | 2025 |
| Saran Enterprise Ltd | Wholesale & retail | 2020 |
| Saran Fasteners Limited | Wholesale & retail | 2022 |
| Sarz Ltd | Real estate | 2025 |
| SAS Hospitality Group Ltd | Hospitality | 2026 |
| Sasdigitals Ltd | Wholesale & retail | 2022 |
| Sattar Family Company Ltd | Real estate | 2025 |
| Sattva Mart Scotland Limited | Wholesale & retail | 2026 |
| Saxingh Ltd | Information & communication | 2022 |
| SB1 Foods Ltd | Hospitality | 2014 |
| Scbs Holdings Limited | Professional & scientific | 2021 |
| Scgp Ltd | Information & communication | 2016 |
| Schmack UK Ltd | Hospitality | 2020 |
| Schuh (Holdings) Limited | Financial services | 2004 |
| Schuh Group Limited | Wholesale & retail | 2010 |
| Schuh Limited | Wholesale & retail | 1990 |
| Scmnit Ltd | Information & communication | 2026 |
| Scoffable Ltd | Hospitality | 2012 |
| Scoland Sky Tours & Transfers Ltd | Transport & logistics | 2024 |
| Scoop & Treat Limited | Wholesale & retail | 2025 |
| Scope Branding Limited | Manufacturing | 2018 |
| Scot Bucket List Limited | Wholesale & retail | 2021 |
| Scot Highlanders Tours and Travels Private Ltd | Administrative & support | 2025 |
| Scot-Signs Limited | Manufacturing | 1997 |
| Scotia Clean Teck Limited | Administrative & support | 1990 |
| Scotlock Security Ltd | Administrative & support | 2026 |
| Scotpac International (U.K.) Limited | Extraterritorial | 1969 |
| Scotpat Ltd | Other services | 2015 |
| Scotphone ICS Limited | Information & communication | 2012 |
| Scotpro Limited | Construction | 1994 |
| Scott CVL Ltd | Administrative & support | 2025 |
| Scott Eco Ltd | Construction | 2025 |
| Scott Edinburgh Ltd | Hospitality | 2026 |
| Scott Events Ltd | Administrative & support | 2018 |
| Scott POD Ltd | Hospitality | 2025 |
| Scott Simpson Touring Limited | Arts & recreation | 2016 |
| Scott VAC Ltd | Construction | 2025 |
| Scottish Early Years Association Ltd | Education | 2021 |
| Scryber Development Ltd | Information & communication | 2020 |
| Sdem Taxis Ltd. | Transport & logistics | 2009 |
| SE Property and Investments Ltd | Real estate | 2024 |
| Sea Way Investors Shipping LP | | 2017 |
| Seaharmony LP | | 2016 |
| Sebko Transport Ltd | Transport & logistics | 2022 |
| Secc Health Ltd | Health & social care | 2026 |
| Second Life Textile Ltd | Wholesale & retail | 2012 |
| Sehrin Ltd | Other services | 2025 |
| Select Wealth Managers Ltd. | Financial services | 2014 |
| Sensor-Works Ltd | Manufacturing | 2014 |
| Service.Is Ltd | Administrative & support | 2026 |
| Serving Padel Ltd | Construction | 2024 |
| Serviseas Ltd | Education | 2020 |
| SG 1975 Access Ltd | Other services | 2020 |
| Sgd(Uk) Ltd | Construction | 2024 |
| SGR Property Limited | Administrative & support | 2006 |
| Shadwell Associates Limited | Professional & scientific | 2025 |
| Shandi Barbers Ltd. | Other services | 2015 |
| Shannon Stevenson Ltd | Health & social care | 2018 |