| 1 River Lane Limited | Real estate | 2023 |
| 157 Columbia Road Ltd | Real estate | 2024 |
| 397 Ashley Road Limited | Real estate | 2023 |
| 44 Broad Lane Ltd | Real estate | 2024 |
| 473 Ashley Road Limited | Real estate | 2023 |
| 475 Ashley Road Ltd | Real estate | 2022 |
| 664 Newmarket Road Limited | Real estate | 2023 |
| 666 Newmarket Road Limited | Real estate | 2022 |
| 76 New Haslingfield Limited | Real estate | 2023 |
| Ab&C Consulting Ltd | Professional & scientific | 2021 |
| Ab&C Ventures Limited | Real estate | 2022 |
| Abbott Healthcare Limited | Financial services | 2006 |
| Abbott Recruitment Limited | Other services | 2002 |
| Abcc Holdings Limited | Professional & scientific | 2020 |
| ACM Cambiz Ltd | Professional & scientific | 2024 |
| Amber Lodges Newmarket Road Ltd | Real estate | 2023 |
| Amdw Management Ltd | Real estate | 2025 |
| Annie Oak Limited | Wholesale & retail | 2019 |
| ASH Austin Holdings Ltd | Financial services | 2025 |
| Ashfarm Limited | Real estate | 2003 |
| Ashton Butler Limited | Extraterritorial | 2016 |
| Autoswitch Electronics Limited | Manufacturing | 1995 |
| Avantiv Limited | Information & communication | 2021 |
| Awesome Houses Ltd | Real estate | 2015 |
| Axzyra Cambridge Limited | Wholesale & retail | 2024 |
| Axzyra Holdings Limited | Financial services | 2024 |
| B.A. Caulkett Limited | Manufacturing | 1990 |
| BAC Spares & Equipment Limited | Professional & scientific | 2002 |
| Baythorne Limited | Professional & scientific | 2014 |
| Black LAB Norton Ltd | Financial services | 2013 |
| Blobfish International Limited | Professional & scientific | 2019 |
| Blue Light Blue Music Limited | Professional & scientific | 2007 |
| Blues Property Abington Limited | Real estate | 2024 |
| Bright Sparks Holdings Ltd | Financial services | 2020 |
| Brighter Perspectives Limited | Professional & scientific | 2019 |
| Brogan James Limited | Professional & scientific | 2016 |
| Brooks and Wren Properties Ltd | Real estate | 2020 |
| Buntingford Storage Ltd | Transport & logistics | 2014 |
| BY Nada Fahmy Design Limited | Professional & scientific | 2022 |
| Cam-Era Culture Exchange Limited | Education | 2012 |
| Cam-Sci Knowledge Economy Development Ltd | Professional & scientific | 2014 |
| Cam-Sci Science Parks Ltd | Professional & scientific | 2011 |
| Camal Architects Limited | Professional & scientific | 2015 |
| Cambridge and Country (Horningsea) Ltd | Construction | 2026 |
| Cambridge Architects Limited | Professional & scientific | 2015 |
| Cambridge Carpentry and Construction Ltd | Construction | 2014 |
| Cambridge Copiers Ltd | Manufacturing | 2015 |
| Cambridge Neurology Ltd | Health & social care | 2023 |
| Cambridge Property Developments Ltd | Real estate | 2015 |
| Cambridge Rapid Components Limited | Manufacturing | 1987 |
| Cambridge Technology Research Limited | Professional & scientific | 2015 |
| Camelot Creature Comforts Limited | Wholesale & retail | 2015 |
| Caxton Consulting Ltd | Professional & scientific | 2015 |
| Ccvb Limited | Real estate | 2004 |
| Centimetrics Limited | Professional & scientific | 2013 |
| Centimetrics Research UK Ltd | Professional & scientific | 2014 |
| Change IT Transformation Solutions Ltd | Information & communication | 2014 |
| Charwin Construction Limited | Construction | 2025 |
| Charwin Homes (Great Bromley) Limited | Construction | 2025 |
| Charwin Homes (Houghton Regis) Limited | Construction | 2025 |
| Charwin Homes (Martham) Limited | Construction | 2025 |
| Charwin Homes (Sileby) Limited | Construction | 2025 |
| Charwin Homes Limited | Construction | 2025 |
| Cheyney Arbitration Limited | Professional & scientific | 2020 |
| Cheyney Resources Limited | Professional & scientific | 2014 |
| Chris Dunn Consulting Services Limited | Professional & scientific | 2016 |
| CLK Engineering Limited | Construction | 2013 |
| Clkc Holdings Ltd | Financial services | 2020 |
| Copperfield Holdings Limited | Administrative & support | 2020 |
| Create Developments (Manchester) Limited | Construction | 2016 |
| Cresset Biomolecular Discovery Limited | Information & communication | 2001 |
| Cuckoo Clothing Limited | Wholesale & retail | 2007 |
| Curtis Medical UK Ltd | Professional & scientific | 2014 |
| Cwmt Ltd | Real estate | 2012 |
| D & T Supplies Limited | Wholesale & retail | 2011 |
| D&D Properties and Maintenance Ltd | Construction | 2024 |
| Danlab (Royston) Limited | Health & social care | 2016 |
| Digital Scientific Instruments Ltd | Information & communication | 1992 |
| DJA (Cambridge) Ltd | Professional & scientific | 2014 |
| DMB Investments & Capital Limited | Professional & scientific | 2020 |
| DOA Properties Limited | Real estate | 2016 |
| Dolly Treasures Ltd | Information & communication | 2015 |
| Dormit Property Management Limited | Real estate | 2022 |
| Doyen Land Ltd | Professional & scientific | 2016 |
| Drilles London Ltd | Wholesale & retail | 2016 |
| EAR & Nose Care Limited | Health & social care | 2010 |
| EC3 Music Publishing Ltd | Information & communication | 2015 |
| EC3 Promotions Ltd | Professional & scientific | 2015 |
| EC3 Records Ltd | Administrative & support | 2014 |
| Ecomachines Ventures Limited | Professional & scientific | 2013 |
| Eemi Limited | Health & social care | 2013 |
| Elliott Forensics Ltd | Professional & scientific | 2012 |
| Enigma Automation Ltd | Professional & scientific | 2020 |
| Equity Spark Ltd | Financial services | 2013 |
| ERI Bedwas Limited | Professional & scientific | 2019 |
| Ewington Properties Limited | Real estate | 2022 |
| Excel Medicolegal Training Limited | Education | 2019 |
| Fabbash Management Ltd | Real estate | 2025 |
| Fenlander Software Solutions Limited | Information & communication | 2011 |
| Fidemor Consultancy Limited | Information & communication | 2023 |