| JNL Build Group Ltd | Construction | 2025 |
| JO Hargreaves Therapy and Consultation Limited | Health & social care | 2022 |
| Jobs Joinery & Property Services Ltd | Manufacturing | 2025 |
| Jodie Saunders Ltd | Arts & recreation | 2022 |
| JOE Toyne Drylining Ltd | Construction | 2020 |
| JOE Winter Building Services Limited | Construction | 2005 |
| JOE Winter Holdings Limited | Professional & scientific | 2008 |
| Joes Garden Patch Limited | Agriculture & fishing | 2022 |
| John and Flannery Paving & Kerbing Ltd | Construction | 2021 |
| John Butler Medical Practice Ltd | Health & social care | 2017 |
| John Evans (Entertainments) Limited | Arts & recreation | 1970 |
| John Foster Plumbing & Heating Ltd | Construction | 2012 |
| John Gillespie Magee JR Foundation | Education | 2017 |
| John Greed Group Limited | Financial services | 2010 |
| John Greed Jewellery Limited | Wholesale & retail | 2003 |
| John Halton Design Limited | Professional & scientific | 1995 |
| John Kaberry Limited | Wholesale & retail | 1973 |
| John Lavender Services Ltd | Transport & logistics | 2022 |
| John M. Andrew Limited | Construction | 2005 |
| John Martin-Hoyes (Holdings) Limited | Construction | 2002 |
| John Martin-Hoyes Limited | Construction | 1986 |
| John Mitchell Ltd | Professional & scientific | 2011 |
| John O'Dowd Ltd | Health & social care | 2009 |
| John Pegg Post Ltd | Information & communication | 2019 |
| John Roberts Architects Limited | Professional & scientific | 1985 |
| John ROE Limited | Real estate | 2004 |
| John Seagrief Consultants Limited | Professional & scientific | 2006 |
| John Sharp EYE Surgery Ltd | Health & social care | 2022 |
| John Strang Ltd | Health & social care | 2011 |
| John Ward & Sons Properties Limited | Real estate | 2021 |
| John Ward & Sons Property Maintenance Ltd | Construction | 2024 |
| John Ward Investments Limited | Real estate | 2024 |
| John-Paul Transport Ltd | Wholesale & retail | 2002 |
| Johnson & Smith (Lincoln) Limited | Construction | 2007 |
| Johnson and Gault Construction (UK) Limited | Construction | 2009 |
| Johnson Holdings (Lincoln) Limited | Financial services | 2024 |
| Johnson Packaging Ltd | Manufacturing | 2011 |
| Johnston Brothers Ltd | Transport & logistics | 2012 |
| Johnston Commercials Ltd | Households | 2024 |
| Joia Events Ltd | Hospitality | 2026 |
| Joiners Arms Victoria Street Ltd | Hospitality | 2017 |
| Jointine Products (Lincoln) Limited | Manufacturing | 1999 |
| Jointline Holdings Limited | Professional & scientific | 2005 |
| Jolbad Global Services Limited | Transport & logistics | 2025 |
| Jolee Services Ltd | Wholesale & retail | 2024 |
| Jollification CIC | Education | 2023 |
| Jolly Good Web Ltd | Information & communication | 2020 |
| Jomac Engineering Limited | Manufacturing | 1992 |
| JON Dawson & SON Limited | Transport & logistics | 2012 |
| JON Goulding Signalling Limited | Information & communication | 2013 |
| JON Kirke Plumbing & Heating Limited | Professional & scientific | 2014 |
| JON Ward Limited | Information & communication | 2010 |
| Jonahco Limited | Manufacturing | 2021 |
| Jonam Composites Ltd | Professional & scientific | 2007 |
| Jonathan Goodwin Ltd | Professional & scientific | 2019 |
| Jonathan Marsh Ltd | Transport & logistics | 2025 |
| Jonathan Mills Ltd | Health & social care | 2024 |
| Jonathan Whiting Independent Funeral Directors Limited | Other services | 2016 |
| Jones Dixon Developments Ltd | Construction | 2026 |
| Jones of Bailgate Ltd | Wholesale & retail | 2020 |
| Jonni Ventures Limited | Hospitality | 2014 |
| Jooler Ltd | Wholesale & retail | 2024 |
| Jorge Artisan Foods Ltd | Manufacturing | 2012 |
| Jorge Fine Foods Ltd | Wholesale & retail | 2021 |
| Josh Burn Estates Ltd | Real estate | 2023 |
| Josh Langtry Construction Ltd | Construction | 2018 |
| Josh Thomas Developments Limited | Construction | 2025 |
| Josh's Care Company Ltd | Health & social care | 2019 |
| Joshi Property Group Limited | Real estate | 2022 |
| Joshua Chambers Trading Ltd | Wholesale & retail | 2025 |
| Josiriam Ltd | Transport & logistics | 2023 |
| Joslin Media Ltd | Extraterritorial | 2025 |
| Jotslo Ltd | Information & communication | 2024 |
| Joy Odili Plastic Surgery Ltd | Health & social care | 2025 |
| Joy Tender Loving Care Services Limited | Hospitality | 2023 |
| Joy UK (Translation & Interpretation) Ltd | Professional & scientific | 2003 |
| Joy Walker Estate Agents Limited | Real estate | 2005 |
| JP Espin Consultancy Ltd | Professional & scientific | 2025 |
| JP Exterior Cleaning Ltd | Administrative & support | 2021 |
| JP Jones Contracting Ltd | Transport & logistics | 2026 |
| JP Maher Ltd | Construction | 2026 |
| JPC Management Consultancy Limited | Professional & scientific | 2018 |
| JPC Property Investment Ltd | Real estate | 2019 |
| JPW (Holdings) Ltd | Real estate | 2017 |
| JPW Property Solutions Ltd | Real estate | 2021 |
| JPW Trade Limited | Construction | 2021 |
| JR and AD Howell Ltd | Health & social care | 2010 |
| JR Cleaning and Gardening Service Ltd | Administrative & support | 2021 |
| JR Developments Lincoln Ltd | Construction | 2024 |
| JR Glass & Glazing Limited | Construction | 2012 |
| JRC 23 Limited | Professional & scientific | 2019 |
| JRC Commercial Property Ltd | Real estate | 2025 |
| JRC Residential Ltd | Real estate | 2022 |
| JRF Financial Planning Limited | Financial services | 2017 |
| JRH Consultancy Limited | Information & communication | 2007 |
| JRH Electrical Contracting Ltd | Construction | 2016 |
| JRL Consulting Ltd | Professional & scientific | 2024 |
| Jrrm Holdings Limited | Financial services | 2023 |
| Jrrm Limited | Professional & scientific | 2008 |
| JS Auto Solutions Limited | Wholesale & retail | 2025 |