| Unique Homecare Lincoln Ltd | Health & social care | 2020 |
| Unique Paving Limited | Construction | 2014 |
| Unique Plastics Limited | Wholesale & retail | 2004 |
| Unit 8 Minis Ltd | Wholesale & retail | 2025 |
| United Agency Limited | Information & communication | 2022 |
| United Children's Services Limited | Health & social care | 2011 |
| United Education Services Limited | Education | 2018 |
| United Health Group 2 Limited | Financial services | 2019 |
| United Management and Consultancy Services Ltd | Professional & scientific | 2018 |
| Unity Acquisitions Limited | Real estate | 2008 |
| Unity Property Holdings Limited | Real estate | 2017 |
| Unity Sphere Ltd | Wholesale & retail | 2025 |
| University of Lincoln Dental Services CIC | Health & social care | 2025 |
| University of Lincoln Students' Union | Administrative & support | 2007 |
| Unleash Sales Limited | Professional & scientific | 2021 |
| Unlikely Productions Limited | Arts & recreation | 2005 |
| UNO Accountancy Services Limited | Professional & scientific | 2018 |
| UNO Plus Ltd | Professional & scientific | 2026 |
| Unstoppabl Consulting Ltd | Extraterritorial | 2024 |
| UOL Services Limited | Education | 2017 |
| UP Civils and Groundworks Ltd | Construction | 2019 |
| UP Construction Limited | Construction | 2016 |
| UP FLY Soft Ltd | Wholesale & retail | 2022 |
| UP the Junxion Ltd. | Hospitality | 2017 |
| Uphill Lincoln Lettings Ltd | Real estate | 2019 |
| Upland Park Medical Ltd | Health & social care | 2018 |
| Upright & Balanced Ltd | Wholesale & retail | 2023 |
| Urban Angels Hair Ltd | Other services | 2021 |
| Urban Apache Films Limited | Information & communication | 2011 |
| Urban Blinds Ltd | Wholesale & retail | 2021 |
| Urban Dynamics Ltd | Professional & scientific | 2023 |
| Urban Games Limited | Financial services | 2024 |
| Urban Hair Ltd | Other services | 2023 |
| Urban Moto Ltd | Manufacturing | 2011 |
| Urban Upgrades Ltd | Construction | 2025 |
| Urobott Ltd | Health & social care | 2021 |
| Urolaris Ltd | Health & social care | 2022 |
| Urology Partners LLP | | 2010 |
| Uroscreen Ltd | Health & social care | 2024 |
| USE IT OR Lose IT Ltd | Extraterritorial | 2004 |
| USE IT OR Lose IT Security Systems Ltd | Extraterritorial | 2004 |
| Usmazon UK Ltd | Wholesale & retail | 2020 |
| Utility Solutions Consultancy Ltd | Professional & scientific | 2024 |
| Uttley Engineering Services Ltd | Professional & scientific | 2025 |
| V Mahajan Limited | Health & social care | 2018 |
| V2 Services Ltd | Construction | 2026 |
| VA PPM Ltd | Professional & scientific | 2015 |
| Vaayu Limited | Wholesale & retail | 2024 |
| Vaddaram Ltd | Real estate | 2017 |
| Vaddaram Pro LLP | | 2017 |
| VAG Auto Care Services Ltd | Wholesale & retail | 2021 |
| Valentino Pizzeria Limited | Hospitality | 2020 |
| Valentinos Food Ltd | Hospitality | 2025 |
| Valhalla Floors Ltd | Construction | 2025 |
| Valley Cottage Developments Ltd | Construction | 2017 |
| Valtrans (Lincoln) Limited | Transport & logistics | 2018 |
| Vane Consulting Ltd | Professional & scientific | 2025 |
| Vanquip UK Ltd | Wholesale & retail | 2024 |
| Vantage Point Photography Ltd | Professional & scientific | 2025 |
| Vantagekey Property Limited | Real estate | 2025 |
| Vape (Worksop) Ltd | Wholesale & retail | 2014 |
| Vapour Modified Components Limited | Professional & scientific | 2022 |
| Varanasi Navenby Ltd | Hospitality | 2026 |
| Vark Estates Ltd | Real estate | 2019 |
| VAS Haulage Ltd | Transport & logistics | 2022 |
| Vaughn S Mottram Solutions Ltd | Professional & scientific | 2019 |
| Vault Biotech Ltd | Wholesale & retail | 2025 |
| Vauxco Limited | Professional & scientific | 2017 |
| Vawtex Ltd | Manufacturing | 2025 |
| VB Equestrian Ltd | Education | 2021 |
| Vchib Ltd | Health & social care | 2025 |
| Veal Media Limited | Information & communication | 2007 |
| Vehicle Electrix Limited | Wholesale & retail | 2015 |
| Velicko Transport Ltd | Transport & logistics | 2018 |
| Velocette Ltd | Other services | 2008 |
| Venari Fuel Ltd | Real estate | 2020 |
| Vend Cube Ltd | Wholesale & retail | 2024 |
| Venim Limited | Wholesale & retail | 2017 |
| Venochek Limited | Health & social care | 2017 |
| Venom Cars Ltd | Wholesale & retail | 2022 |
| Venom Properties Ltd | Real estate | 2023 |
| Venture Signs & Graphics Ltd. | Other services | 2004 |
| Ventureflowapp Limited | Information & communication | 2025 |
| Venus Safety Ltd | Information & communication | 2025 |
| Veritas Bathrooms Limited | Construction | 2015 |
| Veritas Mundi Education Ltd | Administrative & support | 2023 |
| Veritus Ltd | Other services | 2025 |
| Vermari Milano Limited | Wholesale & retail | 2026 |
| Vermillion Group Ltd | Other services | 2015 |
| Versatilis Enterprise Limited | Information & communication | 2023 |
| Versusi Limited | Wholesale & retail | 2023 |
| Vetnet Europe Limited | Wholesale & retail | 1995 |
| Vetra Nova Limited | Real estate | 2025 |
| Veynor CSB Ltd | Professional & scientific | 2023 |
| VFR Hub Ltd | Wholesale & retail | 2025 |
| VG Sports Foundation CIC | Education | 2026 |
| Vgct 42 Ltd | Arts & recreation | 2025 |
| VHF (Navenby) Ltd | Real estate | 2006 |
| VIA Della Vita Ltd | Hospitality | 2024 |
| Viacerta Transport Consulting Ltd | Professional & scientific | 2026 |