| Drywood Associates Ltd | Households | 2026 |
| DSC Consultancy Limited | Professional & scientific | 2009 |
| Dtech IT Limited | Information & communication | 2008 |
| DTJ Voice & Data Ltd | Information & communication | 2003 |
| DTJ Electrical Limited | Construction | 2000 |
| Dunkirk Garage Limited | Wholesale & retail | 2003 |
| Dunne & Pilkington Limited | Professional & scientific | 2002 |
| Dunnes Roofing Specialists Ltd | Construction | 2020 |
| Duxbury's Delivery Services Ltd | Transport & logistics | 2025 |
| DVC Consulting Limited | Professional & scientific | 2025 |
| Dwyer Burton Ltd | Information & communication | 2015 |
| Dynamic Properties NW Limited | Real estate | 2025 |
| Dynamic Roofing & Building Contractors Limited | Construction | 2022 |
| Dynamite Nutrition Limited | Wholesale & retail | 2021 |
| E N J Prestige Ltd | Wholesale & retail | 2023 |
| E&H Property Partners Limited | Real estate | 2025 |
| E-Commerce North West Ltd | Wholesale & retail | 2019 |
| Eagle and Child Ltd | Extraterritorial | 2019 |
| Eagle EYE Limited | Manufacturing | 1981 |
| Earnshaw Manor LLP | | 2021 |
| East Coast Power Solutions Limited | Construction | 2025 |
| Easy Shop Leyland Ltd | Wholesale & retail | 2025 |
| Easy Wash Linen Ltd | Other services | 2024 |
| Eazi-Grip Racing Products Limited | Wholesale & retail | 2011 |
| EB Autos Ltd | Wholesale & retail | 2023 |
| Ebowlingandsons Ltd | Water & waste | 2025 |
| Eccleston Aviation Limited | Administrative & support | 1996 |
| Eccleston Scaffolding Services Ltd | Construction | 2024 |
| Eco Recycling (UK) Limited | Water & waste | 2010 |
| Edair Engineering Ltd | Manufacturing | 1996 |
| Eddytech Solutions Ltd | Professional & scientific | 2025 |
| Edge IT Support Limited | Information & communication | 2020 |
| Edifyxgroup Ltd | Wholesale & retail | 2025 |
| Educational Design Services Limited | Professional & scientific | 1998 |
| Edwards Development Services Ltd | Real estate | 2021 |
| Efes Leyland Ltd | Hospitality | 2024 |
| Efes Turkish Cuisine Leyland Ltd | Hospitality | 2026 |
| Eir-Global Ltd | Professional & scientific | 2024 |
| EJ Golf Gloves Limited | Wholesale & retail | 2021 |
| Ejide Heirs Holdings Ltd | Financial services | 2023 |
| EK LUX Concierge Limited | Wholesale & retail | 2025 |
| EL Tano Wines Ltd | Hospitality | 2024 |
| Elastomer Engineering Limited | Manufacturing | 1966 |
| Elderberry Publishing Limited | Information & communication | 2025 |
| Elegant Bedrooms Limited | Wholesale & retail | 2020 |
| Elemcaza Limited | Financial services | 2025 |
| Element 3 Design Limited | Administrative & support | 2016 |
| ELEV8ED HR Ltd | Professional & scientific | 2025 |
| Elevate Accounting Services Ltd | Professional & scientific | 2026 |
| Elevate Construction Group Ltd | Real estate | 2021 |
| Elevate Consultancy Services NW Ltd | Professional & scientific | 2025 |
| Elevate M Group Ltd | Professional & scientific | 2025 |
| Elevate Management Group Limited | Financial services | 2025 |
| Elevate Physique Coaching Limited | Administrative & support | 2023 |
| ELH Property (NW) Ltd | Real estate | 2025 |
| ELH Systems Ltd | Construction | 2023 |
| Elisavet Ltd | Arts & recreation | 2020 |
| Elite Homes Development Limited | Real estate | 2025 |
| Elite Site Management Ltd | Administrative & support | 2026 |
| Elitebedrooms Ltd | Wholesale & retail | 2017 |
| Elkruk Medical Limited | Health & social care | 2025 |
| Ellis Developments (PC) Limited | Construction | 2025 |
| Ellis X Prism Limited | Real estate | 2022 |
| Ellr (NW) Limited | Construction | 2024 |
| Elmore Berry Ltd | Wholesale & retail | 2025 |
| Elstr Ltd | Wholesale & retail | 2025 |
| Elton Swift Ltd | Wholesale & retail | 2025 |
| Embassy Company Management Ltd | Information & communication | 2025 |
| EMC Consultancy Services LLP | | 2025 |
| EMH Optical Ltd | Professional & scientific | 2016 |
| Emilianomnavarro Tattoo Limited | Arts & recreation | 2025 |
| Emily Robyn Nail Artist Limited | Other services | 2026 |
| Emjayne Ltd | Transport & logistics | 2023 |
| Empowered Generations Ltd | Education | 2023 |
| Energy Savings Plus Limited | Administrative & support | 2025 |
| Energy Support Solutions Ltd | Professional & scientific | 2024 |
| Engaging Little Apps Limited | Information & communication | 2025 |
| Enlightened Lamp Recycling Limited | Water & waste | 2004 |
| Enterprize Web Design and Print Limited | Manufacturing | 2013 |
| Enthrall Health Limited | Health & social care | 2024 |
| Envioex Ltd | Wholesale & retail | 2025 |
| Envirocare Grounds Maintenance (Preston) Limited | Administrative & support | 2009 |
| Envirocare Machinery & Hire Limited | Manufacturing | 2026 |
| Envirocare Maintenance Solutions Limited | Administrative & support | 2005 |
| Environ Hoard Ltd | Construction | 2014 |
| Epic Builds (NW) Ltd | Construction | 2021 |
| Epitaph Limited | Transport & logistics | 2021 |
| Equestrifun Ltd | Wholesale & retail | 2018 |
| Eridom Limited | Construction | 2013 |
| ESG Helix Ltd | Administrative & support | 2024 |
| Espe Ltd | Education | 2018 |
| Essential Racing Ltd | Real estate | 2020 |
| Eternal Renewable Energy Ltd | Construction | 2023 |
| Eternity Woodland Limited | Other services | 2020 |
| Ethel Nelson Properties Ltd | Real estate | 2024 |
| Etrain Limited | Education | 2014 |
| Euro Football Services U.K. Limited | Arts & recreation | 1994 |
| Eurosafe Windows Limited | Construction | 2018 |
| Eutopia Studios Ltd | Wholesale & retail | 2024 |
| Evans and Mather Limited | Hospitality | 2018 |