| Curlew Dairy Company Ltd | Manufacturing | 2021 |
| D G & M Construction Limited | Construction | 2012 |
| D T Dinsdale & SON Limited | Agriculture & fishing | 2025 |
| D&S Spence and SON | Agriculture & fishing | 2025 |
| D. P. Harmsworth (Joinery & Building) Limited | Construction | 2009 |
| D.M. Electronics Ltd | Manufacturing | 1992 |
| Dale Garth Ltd | Construction | 2020 |
| Dales Dance Ltd | Arts & recreation | 2004 |
| Dales Glazing Solutions Ltd | Construction | 2020 |
| Dalesport Sporting Agency LLP | | 2014 |
| Dartmoor Union Cider Press Limited | Households | 2026 |
| Delivered Gifts Limited | Wholesale & retail | 2015 |
| Delivered Services Limited | Other services | 2015 |
| Dent Brothers Services Ltd | Wholesale & retail | 2026 |
| Diamond Lane Doodles Ltd | Agriculture & fishing | 2011 |
| Digitamation Ltd | Wholesale & retail | 2007 |
| DIM SUM Digital Ltd | Information & communication | 2012 |
| Dspb Limited | Information & communication | 2011 |
| Eastfield Game Farm Limited | Agriculture & fishing | 2008 |
| Eastfield Glamping Farm Ltd | Hospitality | 2022 |
| EAT Telfit Ltd | Wholesale & retail | 2023 |
| Eaton Assets Ltd | Financial services | 2018 |
| Eaton Contractors Ltd | Construction | 2018 |
| Eaton Property Ltd | Real estate | 2016 |
| Eddie OIL & GAS Engineering Services Limited | Administrative & support | 2013 |
| Edgley Garage Ltd | Wholesale & retail | 2025 |
| EF Consultancy (Coverdale) Limited | Professional & scientific | 2024 |
| EL Entertainment Limited | Information & communication | 2015 |
| Elaine Phillips Antiques Limited | Wholesale & retail | 1973 |
| Elite Apartments Management Company Limited | Real estate | 2013 |
| Elite Squad Laser TAG Ltd | Arts & recreation | 2021 |
| Emotional Health Limited | Health & social care | 2008 |
| EMS Optom Ltd | Health & social care | 2022 |
| Fairdrive Limited | Wholesale & retail | 1992 |
| Fairhursts Catering Limited | Hospitality | 2017 |
| Farming Fencing Landscaping Contractors (FFL) Ltd | Agriculture & fishing | 2015 |
| Farmoor Services LLP | | 2006 |
| Filpro Consultancy Limited | Information & communication | 1987 |
| Finoteca Ltd | Arts & recreation | 2017 |
| Fionix Ltd | Professional & scientific | 2016 |
| Flowfast Limited | Information & communication | 1997 |
| Foulrice Park Racing Limited | Arts & recreation | 2009 |
| Foxtrot Racing Management Ltd | Arts & recreation | 2009 |
| FS AI Technologies Limited | Financial services | 2025 |
| G Barnard Limited | Construction | 2019 |
| G R Bland & Company Limited | Professional & scientific | 2010 |
| G. N. Thwaite Heating & Plumbing Limited | Construction | 2009 |
| Gavin Smith Building and Landscaping Ltd | Construction | 2014 |
| Gayle Mill Trust | Arts & recreation | 2004 |
| Genesis PL Ltd | Financial services | 2021 |
| Genesis Protection Services Ltd | Financial services | 2017 |
| George Andrew Dental Ltd | Health & social care | 2025 |
| Gill Edge Barn Limited | Real estate | 2006 |
| Glow & DRY UK Limited | Administrative & support | 2020 |
| Goldsmith House (Leyburn) Management Company Limited | Households | 2004 |
| Granby Fine Arts Limited | Wholesale & retail | 1967 |
| Grandparents Voice LLP | | 2015 |
| Greenwood Farm Contracting Limited | Agriculture & fishing | 2017 |
| Gtec Construction Ltd | Construction | 2018 |
| Gtec Property Holdings Ltd | Real estate | 2018 |
| Gunn Technology Limited | Real estate | 2009 |
| H Wood & Sons (Leyburn) Limited | Real estate | 1957 |
| H. Binks & Sons Limited | Transport & logistics | 1963 |
| Hackness Sporting Society 2020 Limited | Agriculture & fishing | 2020 |
| Hague Estates Ltd | Real estate | 2025 |
| Haighfield Country Park Limited | Hospitality | 2025 |
| Hall ING Properties Ltd | Real estate | 2025 |
| Harmonic Resonance EE System Ltd | Other services | 2023 |
| Harrisbrook Consultancy Limited | Professional & scientific | 2005 |
| Hawes Ice Cream Parlour Ltd | Hospitality | 2022 |
| Heritage Cards and Souvenirs Limited | Wholesale & retail | 2010 |
| High Cotterdale Sporting Society Limited | Agriculture & fishing | 2002 |
| Hollrose Ltd | Real estate | 2019 |
| Holmedale PRE School Ltd | Health & social care | 2013 |
| Hoppers Removals Limited | Transport & logistics | 2008 |
| Horns (Castle Bolton) Limited | Agriculture & fishing | 1996 |
| Howard Agriculture Ltd | Agriculture & fishing | 2019 |
| Hugh Crawford Property Ltd | Real estate | 2005 |
| Hybrid Property Care Ltd | Administrative & support | 2023 |
| I.O. Workshops Limited | Information & communication | 1983 |
| Ibbidirect Limited | Wholesale & retail | 2015 |
| Ideal Changes Limited | Construction | 2019 |
| Innkeypurrs Limited | Hospitality | 2026 |
| Inspiration-All Limited | Professional & scientific | 2019 |
| Integrated Polymer Systems (U.K.) Limited | Wholesale & retail | 1991 |
| Interprint Services Limited | Manufacturing | 2024 |
| Intravyn Ltd | Information & communication | 2026 |
| Intrinsic Limited | Administrative & support | 2017 |
| ISB Catering Limited | Hospitality | 2019 |
| J a Dent & Sons Limited | Agriculture & fishing | 2011 |
| J Ellerton Groundworks Ltd | Construction | 2021 |
| J F Hudson Ltd | Wholesale & retail | 2014 |
| J Parfitt Plumbing & Heating Limited | Construction | 2011 |
| J R & L a Alderson Ltd | Professional & scientific | 2024 |
| J R Scarr Plant Hire Limited | Construction | 2014 |
| J.K. Fanning Limited | Arts & recreation | 2003 |
| J.R. Hopper & Co. (Property Services) Limited | Administrative & support | 1997 |
| James Herriot Co. Limited | Hospitality | 2013 |
| JAN Linsley Education Consultancy Ltd | Education | 2011 |
| JDR Supplies Ltd | Wholesale & retail | 2012 |