| Synnoclin Consulting Ltd | Professional & scientific | 2024 |
| Systems Leadership Community Interest Company | Professional & scientific | 2022 |
| Sēon Creative Ltd | Professional & scientific | 2018 |
| T Bonnar Ltd | Information & communication | 2023 |
| T Hooker Construction and Landscaping Ltd | Construction | 2023 |
| T J Evans Ltd | Professional & scientific | 2003 |
| T.I Property Limited | Real estate | 2020 |
| T.N.Lawrence & SON Limited | Wholesale & retail | 1963 |
| T.W. Plummer & SON Limited | Transport & logistics | 1965 |
| TAC Electrical Contractors Ltd | Construction | 2023 |
| Taffsound Limited | Arts & recreation | 2003 |
| Taigatec Ltd | Information & communication | 2024 |
| Taith Coffee Limited | Hospitality | 2021 |
| Talbot Developments (Sussex) Ltd | Construction | 2020 |
| Talent Network Limited | Administrative & support | 2006 |
| Talkshow CIC | Arts & recreation | 2022 |
| Tammy Tawadros Ltd | Administrative & support | 2016 |
| Tanya Borowski Ltd | Other services | 2017 |
| Tarmin, Inc | | 2018 |
| Tatty Matts Limited | Wholesale & retail | 2014 |
| TC Knill James Limited | Professional & scientific | 2024 |
| Tckj Trustees Limited | Professional & scientific | 2022 |
| TDH Aviation Limited | Transport & logistics | 2021 |
| Teaching and Learning Collaboration LLP | | 2011 |
| Teachweb Limited | Construction | 1998 |
| Team Beauty Limited | Other services | 2009 |
| Team Impact Limited | Professional & scientific | 2025 |
| Team Shah Ltd | Administrative & support | 2019 |
| Technique Ltd | Professional & scientific | 2026 |
| Teen Tips Ltd | Education | 2017 |
| Tell M Limited | Information & communication | 2014 |
| Tellus Studio Ltd | Information & communication | 2024 |
| Telscombe Cliffs Way Management Company Limited | Households | 1992 |
| Temar Limited | Wholesale & retail | 2017 |
| Temple & Shian Events Limited | Hospitality | 2011 |
| Terrapin TV Limited | Information & communication | 2008 |
| Thane House Properties Limited | Real estate | 1984 |
| THE Alternative Limb Project Limited | Manufacturing | 2011 |
| THE Association of Acting Coaches and Educators United Kingdom (Aace UK) CIC | Education | 2025 |
| THE Augmented Text Company Ltd | Information & communication | 2016 |
| THE Avenue (Lewes) Management Company Limited | Extraterritorial | 2013 |
| THE Bevern Trust | Health & social care | 2003 |
| THE Big Bear PUB Company Ltd | Hospitality | 2019 |
| THE Blue Sky Properties NO. 2 Partnership | | 2005 |
| THE Blue Sky Properties NO.1 Partnership | | 2005 |
| THE British Bazadaise Cattle Society | Agriculture & fishing | 1989 |
| THE Cambridge Boathouse Company Limited | Real estate | 2002 |
| THE Chancery Lane Project | Professional & scientific | 2022 |
| THE Changing Workplace Limited | Administrative & support | 1998 |
| THE Chap Limited | Information & communication | 2007 |
| THE Charleston Trust (Bloomsbury in Sussex) | Arts & recreation | 2004 |
| THE Chip Works Limited | Administrative & support | 2016 |
| THE Community Project Ltd. | Households | 1996 |
| THE Dance Academy CIC | Arts & recreation | 2015 |
| THE Dean School of Art Ltd | Education | 2020 |
| THE Fernhill Trust | Professional & scientific | 2022 |
| THE Filming Group Ltd | Professional & scientific | 2017 |
| THE Fine Food Angel Ltd | Wholesale & retail | 2016 |
| THE Firle Sporting Club Limited | Arts & recreation | 2023 |
| THE Fonthill Foundation | Education | 1974 |
| THE Forge Reformer Ltd | Other services | 2025 |
| THE Foundation Stage Forum Limited | Education | 2006 |
| THE Free From Food Company Limited | Hospitality | 2021 |
| THE Garnered Ltd | Wholesale & retail | 2015 |
| THE Glamour Club CIC | Hospitality | 2025 |
| THE Goldman & Weinberg Partnership Ltd | Wholesale & retail | 2018 |
| THE Grange Management Co. (Lewes) Limited | Administrative & support | 1995 |
| THE Greek House Interiors Limited | Wholesale & retail | 2022 |
| THE Green Man's Garden Limited | Administrative & support | 2017 |
| THE Grid Ltd | Arts & recreation | 2026 |
| THE Hand Made Door Company Limited | Construction | 2008 |
| THE IT Girl Sussex Ltd | Information & communication | 2018 |
| THE Juggs Inn Ltd | Hospitality | 2024 |
| THE Letting Landlords Ltd | Real estate | 2024 |
| THE Lewes Co-Operative Learning Partnership | Education | 2014 |
| THE Lewes Pound Community Interest Company | Financial services | 2009 |
| THE Little Green Pet Shop Ltd | Manufacturing | 2022 |
| THE Little Powerful Company Ltd | Health & social care | 2025 |
| THE London Mural Company Ltd | Extraterritorial | 2025 |
| THE Long MAN Inn Limited | Hospitality | 2021 |
| THE Maples Ringmer (Freehold) Limited | Professional & scientific | 2004 |
| THE Metis Organisation Limited | Education | 2026 |
| THE Naked Land Company Limited | Real estate | 2023 |
| THE NET Zero House Ltd | Construction | 2023 |
| THE Oak Designs Company Ltd | Construction | 2008 |
| THE Oak Newick Ltd | Hospitality | 2025 |
| THE Old Post Office Mews Residents Association Limited | Households | 2005 |
| THE Old Racecourse Limited | Real estate | 2021 |
| THE Organisational Fingerprinting Consultancy Ltd | Professional & scientific | 2014 |
| THE Other Hive Ltd | Education | 2018 |
| THE Outdoor Teacher Limited | Education | 2020 |
| THE Pantry (Newick) Limited | Hospitality | 2016 |
| THE Pavilion Trading Company Limited | Extraterritorial | 2014 |
| THE Peasant Group Limited | Wholesale & retail | 2016 |
| THE Pet Agency Ltd | Administrative & support | 2022 |
| THE Pet Store (Ringmer) Limited | Wholesale & retail | 2018 |
| THE Plain Numbers Project Limited | Professional & scientific | 2020 |
| THE Railway Land Wildlife Trust Limited | Education | 1988 |
| THE Ranmore Group Ltd. | Financial services | 2020 |
| THE RAW Partnership LLP | | 2016 |