| Glenrothes Smile Centre Ltd | Health & social care | 2025 |
| Glenview Caravan Park Limited | Real estate | 2013 |
| Goat Marketing Ltd | Information & communication | 2023 |
| Golden EGG Compliance Ltd | Information & communication | 2025 |
| Golf City Taxis Ltd | Transport & logistics | 2013 |
| Golfed Store Ltd | Wholesale & retail | 2025 |
| Gordon Eason Electrical Limited | Construction | 2023 |
| Gordon Hunter Transport Limited | Transport & logistics | 2011 |
| Grade a+ Ltd | Education | 2019 |
| Grangemuir Woodland Chalet Park Ltd | Real estate | 2017 |
| Grangeneuk Consulting Limited | Professional & scientific | 2024 |
| Greenbee Landscapes Limited | Construction | 2024 |
| Greetings Store Leven Limited | Wholesale & retail | 2025 |
| Grocerygrove Ltd | Wholesale & retail | 2025 |
| GSP24 Ltd | Wholesale & retail | 2018 |
| H & L H Properties Ltd | Real estate | 2019 |
| H Fish and Chips Limited | Hospitality | 2018 |
| Hadamy Properties Limited | Real estate | 2024 |
| Hairtracker Limited | Information & communication | 2024 |
| Halley Access & Rigging Ltd | Mining & quarrying | 2026 |
| Halley Property Ltd | Households | 2024 |
| HAN Banana Ltd | Professional & scientific | 2022 |
| Happy House Leven Ltd | Hospitality | 2024 |
| Hart Tomlinson Properties Limited | Real estate | 2018 |
| Harvey Willis Limited | Wholesale & retail | 2012 |
| Hawkslaw Development (Leven) Limited | Real estate | 1981 |
| Headz Hair Co Ltd | Other services | 2026 |
| Healwell Health Solutions Ltd | Health & social care | 2023 |
| Heather Wood Holdings Limited | Real estate | 2023 |
| Heavend Property Management Ltd | Real estate | 2025 |
| Heffron Joiners Limited | Construction | 2003 |
| Henry Balfour and Company Limited | | 1896 |
| Hickory Holdings Limited | Financial services | 2021 |
| High Access Rescue Team | Other services | 2004 |
| High Performance Solutions Ltd | Administrative & support | 2019 |
| Hiline Construction Limited | Construction | 2001 |
| Hill Street Dysart Limited | Real estate | 2025 |
| Hoko Design Ltd | Arts & recreation | 2016 |
| Holcamp Investments Ltd. | Real estate | 2004 |
| Holiday Essentials Scotland Limited | Other services | 2014 |
| Hollywood Nails & Spa Leven Ltd | Other services | 2023 |
| Home Innovations (Fife) Limited | Construction | 2014 |
| Home Treasures Ltd | Wholesale & retail | 2015 |
| Home-Start Levenmouth | Professional & scientific | 2005 |
| Homebro Properties Limited | Real estate | 2024 |
| Horizonts Properties Ltd | Real estate | 2021 |
| HSL Solutions Limited | Professional & scientific | 2009 |
| Hush by Emma Ltd | Other services | 2024 |
| Hybrid+ Gym Limited | Arts & recreation | 2026 |
| Iain Duffus Limited | Wholesale & retail | 2005 |
| IAN Barrett Roofing Ltd | Construction | 2021 |
| IAN Thompson Car Sales Ltd | Wholesale & retail | 2019 |
| Ignis Services Ltd | Manufacturing | 2026 |
| Ijdm Technical Services Limited | Professional & scientific | 2011 |
| Ilek Services Ltd | Manufacturing | 2025 |
| Imries Fish Bar & Seafood Ltd | Wholesale & retail | 2025 |
| Incharvie Group Limited | Manufacturing | 2018 |
| Inchbarn Limited | Hospitality | 2003 |
| Independent One 2 One Ltd | Financial services | 2009 |
| Indian Ocean Bangla Ltd | Hospitality | 2025 |
| Infinity Chemicals Ltd | Manufacturing | 2018 |
| Inkdealers Ltd | Other services | 2017 |
| Innes Deliverys Limited | Transport & logistics | 2025 |
| Innovation in Action Ltd | Professional & scientific | 2014 |
| Inspired Breaks Ltd | Health & social care | 2022 |
| Integrity Inspection Ltd | Professional & scientific | 2022 |
| Investworks Ltd | Real estate | 2021 |
| Iorol Transport Ltd | Transport & logistics | 2023 |
| IPS Technology (UK) Limited | Information & communication | 2009 |
| IRC Travel Ltd | Transport & logistics | 2025 |
| ITM Mechanical Services Limited | Manufacturing | 2009 |
| Ivanthoo Ltd | Wholesale & retail | 2025 |
| J & T Joiners Limited | Construction | 2020 |
| J & T Properties (Fife) Ltd | Real estate | 2024 |
| J Douglas Plumbing & Heating Ltd. | Construction | 2012 |
| J Wilson GAS Engineers Limited | Construction | 2017 |
| J&B Weir Investments Ltd | Real estate | 2017 |
| J.C Taxis (Fife) Limited | Transport & logistics | 2023 |
| JAF Academy Limited | Arts & recreation | 2025 |
| JAF Barber Shop Ltd | Other services | 2024 |
| Jambobs Delivery Ltd | Transport & logistics | 2026 |
| James Vallance Fleming Limited | Agriculture & fishing | 1973 |
| JAX Fitness Scotland Limited | Arts & recreation | 2025 |
| Jayderry Engineering Consultants Limited | Real estate | 2012 |
| JB Turbines Limited | Construction | 2025 |
| JBJ Building Services Limited | Construction | 2019 |
| JD Stenic Ltd | Construction | 2011 |
| JDS Inspection Services (Fife) Ltd | Professional & scientific | 2021 |
| Jess and Jinx Ltd | Real estate | 2021 |
| Jetsetr Limited | Information & communication | 2025 |
| JJ Fans Limited | Hospitality | 2023 |
| JLC Construction Services Limited | Construction | 2024 |
| Jmnb Ltd | Administrative & support | 2025 |
| John Kelman Joinery Limited | Construction | 2021 |
| John Williamson Electrical Limited | Construction | 2013 |
| Jolly Spiffing Marketing & Design Ltd | Professional & scientific | 2025 |
| Jonathan Gull Properties Limited | Real estate | 2004 |
| JP NDE Ltd | Professional & scientific | 2025 |
| JS Lloyd Welding Services Ltd. | Construction | 2009 |
| K&J Mechanical Limited | Wholesale & retail | 2019 |