| 0695 Services Ltd | Professional & scientific | 2021 |
| 146 Bricks Ltd | Real estate | 2019 |
| 2 Wheel Developments Ltd | Wholesale & retail | 2019 |
| 2G Events Ltd | Hospitality | 2018 |
| 407 Consulting Limited | Professional & scientific | 2014 |
| A & S Middleton Properties Ltd | Real estate | 2025 |
| A Evans Electrical Ltd | Construction | 2025 |
| A G Bell Security Services Limited | Administrative & support | 2023 |
| A J W Properties (Scotland) Limited | Real estate | 2003 |
| A K Peri Ltd | Hospitality | 2022 |
| A Pony Called Steve Ltd | Wholesale & retail | 2014 |
| A Scott & SON Ltd. | Agriculture & fishing | 2005 |
| A. Gunn Professional Consultancy Limited | Professional & scientific | 2014 |
| A.F. (Fife) Ltd | Manufacturing | 2025 |
| A.L.C Property & Investments Ltd | Construction | 2025 |
| AA & E Eldorado Ltd | Construction | 2025 |
| Aamp Salon Limited | Other services | 2016 |
| Aaron's Tasty Kitchen Limited | Hospitality | 2025 |
| AB Signature Decor Ltd | Construction | 2022 |
| Abbey Developments (Fife) Ltd. | Construction | 2003 |
| ABC Driving School Ltd | Education | 2018 |
| Aberhill Grocer Limited | Wholesale & retail | 2025 |
| Abetatan (Fife) Ltd | Other services | 2020 |
| AC Welding & Inspection Limited | Construction | 2016 |
| Accmc Ltd | Hospitality | 2023 |
| Adapt & Overcome Coaching Ltd | Arts & recreation | 2022 |
| Added Value Accounting Services Ltd | Financial services | 2010 |
| Advanced Sport Innovations Ltd | Wholesale & retail | 2017 |
| Aeternus Tattoo Company Limited | Other services | 2023 |
| AI Outsourcing Limited | Professional & scientific | 2025 |
| Aitken Property Consultancy Limited | Professional & scientific | 2022 |
| AJ Agri Ltd | Other services | 2025 |
| Ajd(Fife) Limited | Wholesale & retail | 2019 |
| AJS Carwash Fife Ltd | Administrative & support | 2019 |
| Alan Moreland Brickwork Limited | Construction | 2018 |
| Alchemy by Ashley Ltd | Wholesale & retail | 2020 |
| Alcra Joinery Ltd | Construction | 2020 |
| Alex Little Funeral Directors Limited | Other services | 2018 |
| Alkyl Energy Limited | Financial services | 2020 |
| ALL African Foods Limited | Wholesale & retail | 2026 |
| Allan Moncrieff Alarms Limited | Administrative & support | 2013 |
| Alsurveys Limited | Professional & scientific | 2023 |
| AMO Painting (Scotland) Limited | Construction | 2021 |
| Amsha Trade Limited | Wholesale & retail | 2021 |
| Amélie S. Duncan Books Ltd | Wholesale & retail | 2026 |
| AND Quality Engineers Limited | Professional & scientific | 2018 |
| Anderson Properties (Fife) Limited | Real estate | 2020 |
| Anderson Services Ltd | Financial services | 2025 |
| Anderson’S Home Services Ltd | Public administration | 2025 |
| Andrew Cook (Builders) Limited | Construction | 1969 |
| Angel 1 (Fife) Limited | Hospitality | 2026 |
| Anstruther Fish Bar Limited | Hospitality | 2004 |
| AO Extra's Ltd | Arts & recreation | 2024 |
| Apdr Containers Ltd | Transport & logistics | 2024 |
| ARB Hire Scotland Limited | Administrative & support | 2015 |
| Arcan Contracts Ltd. | Construction | 2004 |
| Archer Property(Fife) Limited | Real estate | 2025 |
| Archie Dewar's Body Shop Ltd. | Wholesale & retail | 2009 |
| Archie Fleming Ltd | Construction | 2019 |
| Arden House Projects | Health & social care | 2007 |
| ARW Marine Ltd | Agriculture & fishing | 2018 |
| ASH Bookkeeping & Accountancy Services Ltd | Professional & scientific | 2023 |
| Ashburn Caravan Park Ltd | Real estate | 2017 |
| ASJ Marine Limited | Professional & scientific | 2023 |
| Aspire Membranes Limited | Construction | 2009 |
| ASR Stores Ltd | Wholesale & retail | 2021 |
| AST Heating Limited | Manufacturing | 2014 |
| Asthetics by Lynsay Gibson Ltd | Health & social care | 2025 |
| Asuk Trade Ltd | Wholesale & retail | 2015 |
| Au-Cu Refining Ltd | Manufacturing | 2025 |
| Auld Hoose Windygates Limited | Hospitality | 2019 |
| B a Rigger Services Limited | Manufacturing | 2022 |
| B-Clean Cleaning Services Limited | Administrative & support | 2003 |
| Back Street Hospitality Ltd | Hospitality | 2024 |
| Balcombe Business Services Limited | Administrative & support | 2019 |
| Balcurvie Horse Riding Ltd | Arts & recreation | 2019 |
| Banbeath Smile Solutions Ltd | Health & social care | 2025 |
| Barneys Properties Ltd | Real estate | 2025 |
| Base Leven Ltd | Hospitality | 2022 |
| Basic Project Services Limited | Administrative & support | 1998 |
| Be-Included Care & Support Services Ltd | Health & social care | 2025 |
| Beard Property Management Limited | Households | 2021 |
| Bell Preservation Ltd | Professional & scientific | 2020 |
| Belliston Quarry Company Ltd. (the) | Mining & quarrying | 1943 |
| BEN Robinson Real Estate Limited | Real estate | 2025 |
| Best Istanbul Limited | Hospitality | 2019 |
| Birdie Made to Soar Ltd | Wholesale & retail | 2026 |
| Bissett Properties Ltd | Real estate | 2024 |
| Black's Property Holdings Ltd | Real estate | 2018 |
| Blacks Commercials Ltd | Wholesale & retail | 2018 |
| Blairs Wheel Repairs Ltd | Wholesale & retail | 2012 |
| Blue Earth Clean Limited | Manufacturing | 2021 |
| Bobs 24 Hour Taxis Ltd | Other services | 2010 |
| Boiler Breakdown Company Fife Ltd | Construction | 2025 |
| Bolts Leven Ltd | Wholesale & retail | 2013 |
| Bomo Limited | Real estate | 2012 |
| Bonde (Charleton) Farms Limited | Agriculture & fishing | 1968 |
| Bonnyton Property Developments Limited | Construction | 2024 |
| Braehead Engineering Limited | Professional & scientific | 2014 |
| Braeside Landscapes Ltd | Administrative & support | 2022 |