| 60 North Fishing (Shetland) Ltd | Agriculture & fishing | 2015 |
| 60 North Psychology Ltd | Health & social care | 2021 |
| A J Pottinger Limited | Construction | 2019 |
| A. Jarmson (Shetland) Ltd. | Manufacturing | 2004 |
| Ability Shetland Ltd | Arts & recreation | 2000 |
| Adenia Fishing Co. Limited | Agriculture & fishing | 1987 |
| Alison KAY Fishing Company Limited | Agriculture & fishing | 1999 |
| Alpha Solway Limited | Manufacturing | 1997 |
| ANA Bookkeeping Services Ltd | Professional & scientific | 2021 |
| Antares Fishing (Whalsay) Company Limited | Agriculture & fishing | 1988 |
| Aquatech Mechanical Solutions Ltd | Construction | 2023 |
| Artmachine Shetland Ltd | Other services | 2009 |
| Avrella Fishing Company Ltd | Agriculture & fishing | 2015 |
| Avril Thomson Smith Limited | Wholesale & retail | 2024 |
| B. H Builders Ltd | Construction | 2014 |
| Baila Developments (Shetland) Ltd | Construction | 2022 |
| Basta (Shetland) Ltd | Other services | 2019 |
| BE Ready Fishing Company Ltd | Agriculture & fishing | 2019 |
| Bisset and White Builders Ltd | Construction | 2015 |
| BK Marine Limited | Other services | 2006 |
| BML Accounts Limited | Professional & scientific | 2017 |
| Bolts Car Hire Limited | Administrative & support | 1994 |
| BON AMI Fishing Limited | Agriculture & fishing | 2018 |
| Bonnie Isle Fishing Company Ltd | Agriculture & fishing | 2019 |
| Boo-Stanes Limited | Real estate | 2020 |
| BOY Frazer Fishing Co Ltd | Agriculture & fishing | 2022 |
| Braeside Engineering Ltd | Construction | 2026 |
| Brighter Hope Fishing (Shetland) Ltd | Agriculture & fishing | 2022 |
| BTS Industrial Lettings Limited | Real estate | 2024 |
| Burns Lane Clothing Ltd | Wholesale & retail | 2017 |
| CA Electrical Shetland Ltd | Construction | 2023 |
| Charisma Fishing Company Limited | Agriculture & fishing | 1988 |
| Charisma Holding Company Limited | Agriculture & fishing | 2000 |
| Civil and Structural Engineering Shetland Limited | Professional & scientific | 2016 |
| CJK Marine Ltd | Agriculture & fishing | 2024 |
| Cope Ltd. | Education | 1998 |
| Courageous Fishing (Whalsay) Ltd | Agriculture & fishing | 2014 |
| Cunynghame Self Catering Ltd | Hospitality | 2022 |
| D H Marine (Mergi) Limited | Wholesale & retail | 2005 |
| D Robertson Marine Ltd | Agriculture & fishing | 2011 |
| Defiant Fishing (Whalsay) Company Ltd | Agriculture & fishing | 2021 |
| Deltingpharm Ltd | Health & social care | 2010 |
| Diadent Limited | Health & social care | 2023 |
| Dogs Against Drugs (in Memory of John) | Public administration | 2003 |
| Douglas Enterprise Limited | Hospitality | 2013 |
| Douglas Moore Ltd | Construction | 2011 |
| Drilldeep Limited | Other services | 2011 |
| Duendellure Auto Design Ltd | Wholesale & retail | 2025 |
| EDB Groundworks Ltd | Construction | 2026 |
| Equitable Energy Research CIC | Professional & scientific | 2024 |
| Fairway Fishing Ltd | Agriculture & fishing | 2022 |
| Fiskebas Acquisition Limited | Agriculture & fishing | 2025 |
| Fiskebas Fishing Company Limited | Agriculture & fishing | 1996 |
| G I Services (Shetland) Ltd | Agriculture & fishing | 2014 |
| Garriock Bros.Limited | Construction | 1975 |
| Garriock Properties Limited | Construction | 2016 |
| GB Building Centre Limited | Construction | 2016 |
| GFS Marine Ltd | Other services | 2026 |
| Globus Midco Limited | Financial services | 2021 |
| Globus Topco Limited | Financial services | 2021 |
| GNX Limited | Professional & scientific | 2022 |
| Goudies Funeral Directors Limited | Other services | 1994 |
| Grant Cooling Services Limited | Manufacturing | 2021 |
| Grieg Seafood UK Ltd. | Agriculture & fishing | 2014 |
| Guiding Light Fishing Company Limited | Agriculture & fishing | 2008 |
| HA (FIC) | Financial services | 2021 |
| Haraldsson Engineering Ltd | Professional & scientific | 2025 |
| Hawick Haus Limited | Real estate | 2024 |
| Helsin Limited | Real estate | 2022 |
| Hera Retail Ltd | Wholesale & retail | 2020 |
| HPT Limited | Construction | 2022 |
| Hrafn Shetland Limited | Wholesale & retail | 2019 |
| Hydropixel Ltd | Information & communication | 2012 |
| Hytte Ltd | Real estate | 2022 |
| Irvine Developments Shetland Limited | Construction | 2016 |
| Irvine Enterprises Shetland Limited | Real estate | 1992 |
| Island Photo Solutions Limited | Wholesale & retail | 2011 |
| Island Windows & Doors Ltd | Wholesale & retail | 2021 |
| ITS (Shetland) Limited | Wholesale & retail | 2004 |
| J Anderson Fishing Ltd. | Agriculture & fishing | 2003 |
| J.R.J. Shetland Limited | Wholesale & retail | 2001 |
| JGT Partnership LLP | | 2023 |
| JIM Clark Accountancy Ltd. | Professional & scientific | 2011 |
| Kdmac Limited | Hospitality | 2024 |
| Kelvin Anderson Property Limited | Real estate | 2023 |
| KLM Fishing Ltd | Agriculture & fishing | 2025 |
| Kung FU Cuisine Ltd | Hospitality | 2023 |
| Larks Properties Limited | Other services | 2022 |
| LDS (Shetland) Ltd | Wholesale & retail | 2016 |
| Leask Motor Garage (Lerwick) Limited | Wholesale & retail | 1973 |
| Lerwick Autobody Limited | Wholesale & retail | 2012 |
| Lerwick Engineering & Fabrication Ltd. | Manufacturing | 1994 |
| M.R. Gair Ltd | Wholesale & retail | 2014 |
| Mackay Johnson Ltd | Professional & scientific | 2024 |
| Macleod & Maclean Limited | Real estate | 1924 |
| Magnen Ltd. | Other services | 2018 |
| Magno (Shetland) Ltd | Wholesale & retail | 2018 |
| Manson & Mair Enterprises Ltd | Hospitality | 2023 |
| Manson Building & Property Services Ltd | Construction | 2018 |
| Mansons Laundry and Drycleaning Limited | Other services | 2021 |