| Dunn & Dusted Group Ltd | Financial services | 2026 |
| Durbar Trading Limited | Extraterritorial | 1995 |
| E and S Construction Limited | Construction | 2015 |
| Eagle Services UK Limited | Agriculture & fishing | 1999 |
| Eard Island Trading Ltd | Hospitality | 2020 |
| Easthampton Accountancy Services Ltd | Professional & scientific | 2018 |
| Easthouse Developments Ltd | Real estate | 2008 |
| Echo for Extra Choices in Herefordshire Limited | Health & social care | 2002 |
| Edith Patricia Parry Ltd | Agriculture & fishing | 2022 |
| Edward Bulmer Limited | Manufacturing | 2001 |
| Edward Bulmer Natural Paint Limited | Manufacturing | 2017 |
| Edwards Tree Services Ltd | Agriculture & fishing | 2014 |
| Edycyp Ltd | Transport & logistics | 2016 |
| EIGHTEEN73 Limited | Professional & scientific | 2022 |
| ELEV8ION Properties Limited | Real estate | 2023 |
| Elite Vehicle Wrapping (Hereford) Ltd | Wholesale & retail | 2022 |
| Ellmau Ltd | Real estate | 2023 |
| ELM Computer Solutions Limited | Information & communication | 2009 |
| ELM Computer Systems LEO Ltd | Wholesale & retail | 2012 |
| Elms (Eardisland) Management Limited | Households | 1988 |
| Elms Green Woodchip Limited | Manufacturing | 2013 |
| Elumed Limited | Health & social care | 2014 |
| Emerald Blinds & Curtains Limited | Wholesale & retail | 2011 |
| Emerald Interiors Burscough Limited | Wholesale & retail | 2025 |
| Emerald Interiors Retail Limited | Wholesale & retail | 2025 |
| Emerald Interiors Southport Limited | Wholesale & retail | 2025 |
| Emerald Interiors Tarleton Limited | Wholesale & retail | 2025 |
| Emery Geological Limited | Professional & scientific | 2014 |
| Enchanted Boutique Ltd | Wholesale & retail | 2025 |
| Enchanted Holdings Limited | Financial services | 2025 |
| Environmental Research Group Oxford Limited | Professional & scientific | 1985 |
| Essat Law Limited | Professional & scientific | 2015 |
| Euro B 2000 Ltd. | Construction | 2001 |
| Everything IS Energy Ltd | Administrative & support | 2022 |
| Excelclad Limited | Wholesale & retail | 1986 |
| EYE Veterinary Clinic Limited | Professional & scientific | 2014 |
| FAB Fings Ltd | Wholesale & retail | 2024 |
| Fade House LEO Ltd | Other services | 2025 |
| Faifley Limited | Real estate | 1993 |
| Fairhead Trustees Limited | Financial services | 2023 |
| Faithful Recreations Limited | Professional & scientific | 2013 |
| Farm Court (Lucton) Management Limited | Real estate | 1999 |
| Farm to Fert Limited | Manufacturing | 2021 |
| Farmer Britain Ltd | Arts & recreation | 2007 |
| Farnell Premier Coatings Limited | Extraterritorial | 2014 |
| FB Industrial Ltd | Real estate | 2025 |
| Felix Paraplanning Limited | Financial services | 2019 |
| Ferguson Structures Limited | Construction | 2009 |
| FH Dale Developments Limited | Construction | 2021 |
| Fibrelite (North West) Ltd | Information & communication | 1998 |
| Fibrelite Limited | Information & communication | 2010 |
| Finchly Limited | Professional & scientific | 2024 |
| Fish Legal Ltd | Professional & scientific | 2026 |
| Fishers Land Co Ltd | Real estate | 2025 |
| Fishers Sales Direct Ltd | Real estate | 2011 |
| Flmartin Consulting Ltd | Professional & scientific | 2022 |
| Flowclad Limited | Construction | 2016 |
| Flux Media Limited | Information & communication | 2000 |
| FLY Q Limited | Wholesale & retail | 2011 |
| Fontoura Worktops Limited | Manufacturing | 2007 |
| Foot Down Records Ltd | Arts & recreation | 2024 |
| Forbes Ff&E Limited | Extraterritorial | 2008 |
| Forbes Furniture Limited | Professional & scientific | 2008 |
| Forbes Group Limited | Wholesale & retail | 1981 |
| Forbury Orchard Management Company Limited | Households | 2020 |
| Force Tuning Ltd | Wholesale & retail | 2019 |
| Forge Network Consulting Ltd | Information & communication | 2024 |
| Fortron Autotech Ltd | Manufacturing | 2020 |
| Frame Technologies 15 Ltd | Extraterritorial | 2023 |
| Frame Technologies Ltd | Construction | 2015 |
| Frame Technologies Projects Ltd | Extraterritorial | 2023 |
| Frank H.Dale Limited | Manufacturing | 1958 |
| Franklin & Smith Property Investments & Development Ltd | Real estate | 2020 |
| Free Firm Ltd | Real estate | 2022 |
| G & H Ventures Ltd | Professional & scientific | 2003 |
| G & S Longfield Services Ltd | Construction | 2012 |
| G Thomas Properties Ltd | Extraterritorial | 2017 |
| G&S Sports Limited | Arts & recreation | 2015 |
| G.P. Thomas and SON Limited | Construction | 1978 |
| Gable's Limited | Agriculture & fishing | 2005 |
| GAH Agri-Services Limited | Manufacturing | 2024 |
| Garlick Family Property Limited | Real estate | 2018 |
| Gattertop Drinks Limited | Manufacturing | 2020 |
| GB Rentals Limited | Wholesale & retail | 2009 |
| GED Property Management Ltd | Real estate | 2022 |
| Geerlings Limited | Hospitality | 2008 |
| GEM Painting & Decorating Ltd | Construction | 2023 |
| Gent Fairhead Holdings Limited | Professional & scientific | 2002 |
| Gent Fairhead Reserves Limited | Professional & scientific | 1999 |
| Gerrards Auction Rooms Limited | Wholesale & retail | 2012 |
| Giuliani Architects Limited | Professional & scientific | 2000 |
| Glevum Financial Services Limited | Administrative & support | 1981 |
| Glitter Tech Solutions Ltd | Wholesale & retail | 2024 |
| Gloucester and County Site Improvements Limited | Other services | 1964 |
| Glyn Hamer Mill MIX Ltd | Agriculture & fishing | 2005 |
| Golden Threads CIC | Health & social care | 2020 |
| Goldman LUX Limited | Construction | 2025 |
| Good Bros (Timber Merchants) Limited | Manufacturing | 1991 |
| Good Food Distributors Limited | Manufacturing | 2006 |
| Goodwin Alcaston Ltd | Agriculture & fishing | 2009 |