| Ebony Forge Limited | Agriculture & fishing | 2026 |
| EC Edit Ltd | Professional & scientific | 2025 |
| Echo Heart Limited | Information & communication | 2009 |
| Echoheart Holdings Ltd | Wholesale & retail | 2024 |
| Eclipse Fishing Lakes Ltd | Agriculture & fishing | 2023 |
| Eclipse Solar Limited | Construction | 2021 |
| Eco Wash Group Ltd | Administrative & support | 2019 |
| Ecosip Solutions Limited | Wholesale & retail | 2012 |
| Ecoutez Creative Limited | Professional & scientific | 2004 |
| Ecoutez Print Limited | Manufacturing | 1999 |
| Ecovision Limited | Other services | 2019 |
| Eden Electrical Limited | Construction | 2015 |
| Edge Marketing Consultancy Ltd | Information & communication | 2024 |
| EDJ Industrial Services Ltd | Construction | 2020 |
| EDJ Mechanical Services Ltd | Construction | 2019 |
| Edmunds Racing Ltd | Arts & recreation | 2022 |
| Educational Electronics Limited | Extraterritorial | 1982 |
| EDV Investments Ltd | Financial services | 2024 |
| Edwards Beers and Wine Supplies Ltd | Wholesale & retail | 2009 |
| Edwards Beers and Wines (Holdings) Ltd | Wholesale & retail | 2010 |
| Edwards Construction Limited | Construction | 2020 |
| Edwards Machining Services (EMS) Ltd | Manufacturing | 2019 |
| Edwards Surveyors Limited | Professional & scientific | 2004 |
| EE Electrical Maintenance Ltd | Construction | 2023 |
| Effortmark Limited | Information & communication | 1994 |
| EFG Groundworks Ltd | Construction | 2022 |
| EGG Supplies Ltd | Wholesale & retail | 2013 |
| EIO Scaffolding Ltd | Information & communication | 2026 |
| EJ Hospitality and Events Limited | Hospitality | 2020 |
| EJ Transformation & Change Consultancy Ltd | Information & communication | 2020 |
| EJN Lines Ltd | Transport & logistics | 2019 |
| Elaine Nadine Morgan Ltd | Professional & scientific | 2024 |
| Elario Consulting Ltd | Information & communication | 2024 |
| Elderberry Close Management Company Limited | Households | 1982 |
| Elect Automotive Ltd | Wholesale & retail | 2022 |
| Electrical & Building Supplies Ltd | Wholesale & retail | 2022 |
| Electrofire Ltd | Construction | 2023 |
| Electromech Facilities Ltd | Construction | 2014 |
| Element BKS Limited | Construction | 2019 |
| Elephants and Ants Limited | Information & communication | 2018 |
| Elevate Stock Limited | Wholesale & retail | 2025 |
| Eleven Hillfield Road Limited | Households | 1972 |
| ELF Shelf Media Ltd | Information & communication | 2022 |
| Eliflocal Ltd | Professional & scientific | 2025 |
| Eliphaz Ltd | Health & social care | 2024 |
| Elite Realty Ltd | Real estate | 2023 |
| Elite Rugby Limited | Education | 2024 |
| Elixir Strategy Ltd | Administrative & support | 2009 |
| Eliziel Enterprises Ltd | Health & social care | 2025 |
| Elkcorp Limited | Real estate | 2024 |
| ELM Weir Ltd | Transport & logistics | 2024 |
| Elmar Limited | Real estate | 1996 |
| Elms (Leighton Linslade) Residents Association Limited(The) | Households | 1974 |
| Elshenawy Enterprises Co Limited | Construction | 2024 |
| EMA Retreat Ltd | Other services | 2023 |
| EMA Services Ltd | Administrative & support | 2023 |
| Emay Electrical Services Ltd | Construction | 2021 |
| Emberfield Growth Limited | Education | 2025 |
| Emberstech Ltd | Information & communication | 2018 |
| Embleton Way Buckingham Management Limited | Households | 2003 |
| Emcl Ltd | Arts & recreation | 2017 |
| Emendare Properties Limited | Construction | 2014 |
| Emerald Roofing and Property Maintenance Ltd | Construction | 2025 |
| Emerson Grant Holdings Ltd | Construction | 2021 |
| Emily Barber Consulting Ltd | Professional & scientific | 2026 |
| Emily Somers Consulting Limited | Professional & scientific | 2026 |
| Emine Limited | Real estate | 2023 |
| Emotouk Ltd | Wholesale & retail | 2021 |
| Emporium Group Ltd | Professional & scientific | 2019 |
| Empowerinsight Ltd | Information & communication | 2024 |
| EMS Treat Ltd | Hospitality | 2024 |
| EMUS24/7 Limited | Health & social care | 2020 |
| EN Dash Limited | Information & communication | 2016 |
| Enable Access (Holdings) Limited | Professional & scientific | 2014 |
| Enable Access Limited | Manufacturing | 2017 |
| Encapsulite International Limited | Manufacturing | 1964 |
| Encore Football Limited | Administrative & support | 2022 |
| Encore Sports CIC | Health & social care | 2024 |
| END User Services Limited | Information & communication | 2025 |
| Energy Medicine Association Ltd | Extraterritorial | 2023 |
| Enodia Planning Limited | Professional & scientific | 2025 |
| Enodo Limited | Information & communication | 2018 |
| Enright Retail Ltd | Administrative & support | 2026 |
| Enritched Talent Limited | Administrative & support | 2022 |
| Enso 80 Limited | Professional & scientific | 2011 |
| Envirotect Limited | Manufacturing | 2000 |
| Envoq Ltd | Professional & scientific | 2022 |
| EO Tech Ltd | Professional & scientific | 2024 |
| Epiphany Home Solutions Ltd | Construction | 2022 |
| Epiphanytech Ltd | Professional & scientific | 2025 |
| Equilibrium Car Ltd | Wholesale & retail | 2025 |
| Equilibrium Horse Health Limited | Professional & scientific | 2006 |
| Erddy Sparks Ltd | Administrative & support | 2024 |
| Erica Services Ltd | Information & communication | 2021 |
| Erris Point Ltd | Construction | 2025 |
| ERS Property Investments Ltd | Real estate | 2025 |
| ES30 Limited | Information & communication | 2007 |
| Espresso Boutique Limited | Hospitality | 2023 |
| Estone Grange Management Company Limited | Households | 2015 |
| Ethylene Filtration Ltd | Manufacturing | 2023 |