| 23 Real Estate Ltd | Real estate | 2023 |
| 4PROPERTY Consult Ltd | Administrative & support | 2012 |
| 7AG Communications Ltd | Administrative & support | 2026 |
| 7AG Franchise Holdings Ltd | Financial services | 2026 |
| 7AG Properties Ltd | Real estate | 2026 |
| A & a J Ltd | Real estate | 2018 |
| A & S Arnold Properties Limited | Construction | 2016 |
| A 2 Z Ideas Ltd | Hospitality | 2017 |
| A B Construction Staffordshire Ltd | Construction | 2022 |
| A F Corbishley and SON (Transport) Limited | Transport & logistics | 1999 |
| A H Brooks Solicitors Limited | Professional & scientific | 2011 |
| A Hurd Eyecare Ltd | Health & social care | 2026 |
| A J Transport Ltd | Transport & logistics | 2007 |
| A P Holdings Staffs Ltd | Financial services | 2026 |
| A R Machin Limited | Health & social care | 2019 |
| A T Business Support Ltd | Professional & scientific | 2023 |
| A&M Auto and Recovery Ltd | Wholesale & retail | 2025 |
| AB Lifts Limited | Manufacturing | 2025 |
| Abbott Property Developments Limited | Construction | 2004 |
| ABM Worldwide Limited | Information & communication | 2012 |
| Absolute Childrens Services Ltd | Health & social care | 2014 |
| ACB Transport Limited | Transport & logistics | 2014 |
| Accounts for ALL Limited | Professional & scientific | 2008 |
| Acre Exterior Cleaning Ltd | Administrative & support | 2024 |
| Acre Farm Limited | Other services | 2001 |
| Acting UP Arts CIC | Arts & recreation | 2026 |
| Adday Limited | Wholesale & retail | 2021 |
| Africa Roofing UK Ltd | Extraterritorial | 2014 |
| Against the Grain Beer Ltd | Hospitality | 2017 |
| Aidenvi Limited | Information & communication | 2024 |
| AJ Property Construction & Development Ltd | Construction | 2018 |
| Al-Noor Investments Ltd | Real estate | 2019 |
| Alan Ferns Engineering Services Ltd | Professional & scientific | 2022 |
| Alberton Limited | Extraterritorial | 1964 |
| Albion Mill Leek Limited | Other services | 2003 |
| Alex Shaw Ltd | Construction | 2022 |
| Alliance Myactive Wellbeing Limited | Arts & recreation | 2024 |
| Alliance Supplies Limited | Real estate | 1993 |
| Allicio Nutrition Limited | Manufacturing | 2014 |
| AM Windows and Doors (Leek) Ltd | Wholesale & retail | 2020 |
| Amber FOX Investments Ltd | Real estate | 2022 |
| Ameera's Leek Ltd | Hospitality | 2023 |
| Amica Consulting Limited | Professional & scientific | 2022 |
| AMS Civils & Construction Ltd | Construction | 2026 |
| AMY Elizabeth Consulting Ltd | Administrative & support | 2025 |
| AMY Walklate Limited | Professional & scientific | 2017 |
| Ancra International Limited | Extraterritorial | 1972 |
| Anewaira.Com Ltd | Manufacturing | 2020 |
| Anne Morris Limited | Wholesale & retail | 1998 |
| Ansim Holdings Ltd | Professional & scientific | 2018 |
| Antelope Recruitment Consultancy Ltd | Administrative & support | 2016 |
| Antiquario Ltd | Wholesale & retail | 2024 |
| AP Plumbing & Heating (Staffs) Ltd | Construction | 2024 |
| APB Media Ltd | Information & communication | 2012 |
| APB Property Holdings Ltd | Real estate | 2022 |
| Approach Learning (Secondary) Ltd | Education | 2024 |
| Argowheels Ltd | Wholesale & retail | 2023 |
| Arlo Fire Safety Ltd | Administrative & support | 2026 |
| Armillan Limited | Professional & scientific | 2016 |
| Armstrongs Accountants Limited | Professional & scientific | 2013 |
| Ashenhurst Kennels Ltd | Agriculture & fishing | 2021 |
| Ashling Construction Limited | Construction | 1975 |
| Asif Fastfood Ltd | Hospitality | 2026 |
| ASM Holdings & Investments Ltd | Real estate | 2023 |
| ASM Web Solutions Ltd | Information & communication | 2020 |
| ASTECH8 Limited | Information & communication | 2008 |
| ATL Roofing Ltd | Construction | 2025 |
| ATP Crafts Ltd | Manufacturing | 2024 |
| AUK Associates Ltd | Professional & scientific | 2017 |
| Autobarn Staffordshire Ltd | Wholesale & retail | 2018 |
| Awling Ltd | Wholesale & retail | 2021 |
| Axis Agencies and Manufacturing Limited | | 1973 |
| AY UP Duck Aerospace Ltd | Manufacturing | 2024 |
| AYE1 Ltd | Agriculture & fishing | 2025 |
| Aymes International Limited | Manufacturing | 2008 |
| B and B Fuels Ltd | Wholesale & retail | 2015 |
| Babylon Management Company Limited | Real estate | 2007 |
| Bailey Property Holdings Limited | Real estate | 2020 |
| Bailey Services Limited | Professional & scientific | 2001 |
| Balgo Holdings Ltd | Real estate | 2017 |
| Ball Capital Investment Limited | Financial services | 2011 |
| Ball Haye Management Company Limited | Other services | 1998 |
| Barn Investments Ltd | Professional & scientific | 2012 |
| Barnfields Garage Leek Ltd | Wholesale & retail | 2013 |
| Barnowl Bespoke Construction Limited | Construction | 2017 |
| Barweb Contract Services Limited | Professional & scientific | 2012 |
| Base Bar Leek Limited | Hospitality | 2025 |
| Basford Green Limited | Agriculture & fishing | 2014 |
| Bateman Cheddleton Limited | Financial services | 2018 |
| Bateman Family Holdings Limited | Financial services | 2018 |
| Bateman Staffordshire Limited | Financial services | 2023 |
| Bathe HV Services Ltd | Construction | 2025 |
| Battlefield Bird of Prey Centre Ltd | Other services | 2022 |
| Bayleys Engineering Services Ltd | Professional & scientific | 2018 |
| Bbox Food Ltd | Hospitality | 2023 |
| BDF Limited | Construction | 2015 |
| Beans Property Ltd | Real estate | 2023 |
| Bedz Bedzz & Bedrooms Limited | Wholesale & retail | 2006 |
| Beech Avenue Cheddleton (Management Company) Limited | Real estate | 2004 |
| Beer Box Shop Ltd | Wholesale & retail | 2015 |