| Jobstock Tech Limited | Information & communication | 2022 |
| Jocan Ltd | Wholesale & retail | 2022 |
| Jocan Properties Ltd | Real estate | 2026 |
| Jocelyn James Limited | Wholesale & retail | 2024 |
| Jodhpur Limited | Hospitality | 2023 |
| Jodie Bakes Ltd | Manufacturing | 2020 |
| Jodom Ltd | Other services | 2019 |
| JOE Bagshaw Ltd | Hospitality | 2022 |
| JOE Bill Aviation Ltd | Administrative & support | 2021 |
| JOE Browns Ltd | Wholesale & retail | 1990 |
| JOE Driving Limited | Transport & logistics | 2013 |
| JOE Huddlestone (Wetherby) Limited | Arts & recreation | 2004 |
| JOE Ling Catering Limited | Hospitality | 2023 |
| JOE Lovery Golf Coaching Limited | Education | 2022 |
| JOE Malloney Car Valeting & Detailing Ltd | Administrative & support | 2022 |
| JOE Simons Design Limited | Professional & scientific | 2014 |
| JOE Sykes Properties Ltd | Real estate | 2021 |
| Joegardiner Ltd | Information & communication | 2025 |
| Joel Brooks Solutions Limited | Administrative & support | 2025 |
| Joes Multimedia . Ltd | Information & communication | 2018 |
| Joeth World Limited | Health & social care | 2017 |
| Joey Sports Massage Therapy Ltd | Other services | 2019 |
| JOF Gynaecology Limited | Health & social care | 2024 |
| Johal Collections Ltd | Wholesale & retail | 2025 |
| Johdina Sweets Ltd | Wholesale & retail | 2022 |
| John a Dobbins Limited | Real estate | 1980 |
| John Beaumont Limited | Manufacturing | 1990 |
| John Buxton Services Ltd | Professional & scientific | 2013 |
| John C M Limited | Real estate | 2013 |
| John Carrington Design Ltd | Professional & scientific | 2022 |
| John Cooper Properties Ltd | Real estate | 2020 |
| John Curtis & SON Limited | Water & waste | 1898 |
| John Ennis Plumbing & Heating Limited | Construction | 2014 |
| John F Daly Associates Limited | Professional & scientific | 2010 |
| John F Felton and Co Limited | Professional & scientific | 2003 |
| John Grant Properties Limited | Construction | 2026 |
| John Hall Carpets Limited | Wholesale & retail | 2019 |
| John Hart Homes Limited | Real estate | 2016 |
| John Howe & Co Solicitors Limited | Administrative & support | 1998 |
| John King Chains Limited | Manufacturing | 1988 |
| John Lawson Surveys Ltd | Professional & scientific | 2015 |
| John M Lewis & Co Limited | Professional & scientific | 2004 |
| John Maguire (Sports) Ltd | Manufacturing | 2015 |
| John Matthews Properties Limited | Professional & scientific | 1970 |
| John Messenger Haulage Ltd | Transport & logistics | 2011 |
| John Mojsa Coaching & Consulting Ltd | Education | 2021 |
| John Nathan Ltd | Real estate | 2025 |
| John Robinson (Building Services) Limited | Construction | 2002 |
| John Rodriguez Plastering Ltd | Construction | 2007 |
| John Stabler (Textiles) Limited | Wholesale & retail | 1983 |
| John Street Business Centre Limited | Real estate | 2013 |
| John Sullivan Limited | Real estate | 2007 |
| John Tasker Landscapes Limited | Administrative & support | 2005 |
| John Taylor Consulting Limited | Information & communication | 2025 |
| John Taylor Holdings Limited | Professional & scientific | 1987 |
| John Thompson (Gilders) Ltd | Manufacturing | 2021 |
| John Threlfall Limited | Construction | 2004 |
| John W. & S. Dorin Limited | Wholesale & retail | 1981 |
| John Walker Landscape Design Limited | Professional & scientific | 2016 |
| John White & Sons (Curriers) Limited | Manufacturing | 2005 |
| John White & Sons (Holdings) Limited | Manufacturing | 2005 |
| John Wood (Estates) Limited | Real estate | 1978 |
| John Wyatt Limited | Wholesale & retail | 1934 |
| John Yorke Story Limited | Administrative & support | 2016 |
| Johnlo Willben Limited | Health & social care | 2020 |
| Johnny Fontane's Limited | Hospitality | 2011 |
| Johnshmuel Ltd | Professional & scientific | 2026 |
| Johnson & Hopkins Developments Ltd | Real estate | 2021 |
| Johnson Flooring Ltd | Construction | 2020 |
| Johnson Mowat Holdings Limited | Financial services | 2020 |
| Johnson Mowat Planning Limited | Administrative & support | 2018 |
| Johnson Packaging Services Limited | Other services | 2018 |
| Johnson Resolutions Ltd | Financial services | 2018 |
| Johnson's Property Holdings Ltd | Real estate | 2022 |
| Johnston Design Ltd. | Information & communication | 2017 |
| Join Africa UK Limited | Financial services | 2024 |
| Join Energy Ltd | Professional & scientific | 2023 |
| Joined UP Marketing Partners Ltd | Professional & scientific | 2010 |
| Joint Heritage Invest Ltd | Real estate | 2020 |
| Joint Motion Healthcare Limited | Health & social care | 2019 |
| Joint Rising Limited | Real estate | 2023 |
| Joint Working Ltd | Health & social care | 2020 |
| Joissy Ltd | Health & social care | 2023 |
| Jojo Logistics Ltd | Wholesale & retail | 2019 |
| JOJODIVA16 Ltd | Health & social care | 2018 |
| Jojo’S Boutique and Bridal Ltd | Wholesale & retail | 2026 |
| Jolex Travel Ltd | Administrative & support | 2024 |
| Joli Homes Ltd | Real estate | 2025 |
| Jolly Giraffes Day Nursery Limited | Education | 2004 |
| Jolly ME Ltd | Wholesale & retail | 2025 |
| Jolly Panda Ltd | Hospitality | 2025 |
| Jolly Soul Ltd | Information & communication | 2021 |
| Jolo Entertainment Ltd | Arts & recreation | 2026 |
| Jolon 26 Ltd | Information & communication | 2026 |
| Jome's Health Care Limited | Public administration | 2026 |
| JON Conroy Orthopaedics Limited | Health & social care | 2016 |
| JON Kinsey Hairdressing Ltd | Other services | 2017 |
| JON Kinsey Ltd | Other services | 2010 |
| JON Swarbrigg Ltd | Health & social care | 2020 |
| JON Wray Health and Wellbeing Limited | Arts & recreation | 2018 |