| 81 Bristol Road (Gloucester) Limited | Households | 2005 |
| A R Hutson Property Limited | Real estate | 2022 |
| A R Hutson Services Ltd | Professional & scientific | 2017 |
| AA Flowers Limited | Wholesale & retail | 2018 |
| Ampney Park Trading Company Limited | Agriculture & fishing | 1977 |
| AP Cotswold Fencing Ltd | Construction | 2024 |
| AP Tutoring Limited | Education | 2023 |
| Arboreal Tree Care Ltd | Agriculture & fishing | 2024 |
| Archer Carpentry Services Limited | Manufacturing | 2015 |
| Ashben Enterprise Ltd | Professional & scientific | 2025 |
| B Double E Creative & Brand Ltd | Administrative & support | 2018 |
| BAD Records Limited | Manufacturing | 2024 |
| Bare Food Mafia Ltd | Hospitality | 2025 |
| Baxters Farm Barns Management Company Limited | Households | 1997 |
| Beanstalk Limited | Professional & scientific | 2009 |
| Beatrice Knight Property Limited | Real estate | 2024 |
| Black Squid Studios Ltd | Information & communication | 2023 |
| Blink Automotive Events Limited | Other services | 2015 |
| Blue & Boheme Event Floristry Ltd | Wholesale & retail | 2022 |
| Blue & Boheme Florist Limited | Wholesale & retail | 2020 |
| Bohm Homes Limited | Real estate | 2017 |
| Bowmoor Sailing Club | Arts & recreation | 2001 |
| Bridge House Campsite Cotswolds Ltd | Hospitality | 2025 |
| Bruno Wehrle Agriculture Limited | Agriculture & fishing | 2003 |
| C&R Legal Limited | Professional & scientific | 2013 |
| CAP Three Limited | Information & communication | 1997 |
| Capital Consulting Global Ltd | Information & communication | 2015 |
| Captis Intelligence Ltd. | Information & communication | 2017 |
| Carbis Bay Holiday Limited | Hospitality | 2024 |
| Cegg Foods Ltd | Manufacturing | 2025 |
| Chapco Leisure Ltd | Hospitality | 2023 |
| Ciren Limited | Professional & scientific | 2019 |
| Codima Technologies Ltd | Information & communication | 2002 |
| Cohort Fencing Contractors Limited | Construction | 2016 |
| Collett Crane Hire Limited | Wholesale & retail | 1967 |
| Corina Floyd Ltd | Information & communication | 2015 |
| Cotswold Canoe Hire Ltd | Administrative & support | 2019 |
| Cotswold Car Storage Ltd | Transport & logistics | 2024 |
| Cotswold Christmas Shop Limited | Wholesale & retail | 2020 |
| Cotswold Property Limited | Construction | 2017 |
| Cotswold Restorations Ltd | Construction | 2008 |
| Cotswolds Curated Limited | Hospitality | 2025 |
| Cover Construction Company Limited | Construction | 1964 |
| Cover Property Holdings Limited | Construction | 2018 |
| Crown Oak Living Ltd | Hospitality | 2025 |
| Cruk Group Limited | Financial services | 2024 |
| CS Developments Kiwi Ltd | Construction | 2017 |
| Curio Research & Insight Ltd | Professional & scientific | 2025 |
| Daisy Villas Enterprises Limited | Real estate | 2026 |
| Datos Solutions Ltd | Financial services | 2016 |
| Dawes Close Management Company Limited | Households | 1987 |
| Daycall Investments Limited | Administrative & support | 1994 |
| Dentartwork Dental Care Ltd | Health & social care | 2011 |
| Dobson Lyle Consulting Limited | Professional & scientific | 2019 |
| Drone Tech Solutions Ltd | Professional & scientific | 2019 |
| Dwell Architecture UK Limited | Professional & scientific | 2020 |
| Dynamic Goalkeeping Limited | Education | 2025 |
| Edward Roofing Ltd | Construction | 2021 |
| EL Pepito Ltd | Wholesale & retail | 2020 |
| ELF Education Ltd | Professional & scientific | 2018 |
| Elidas Consulting Ltd | Professional & scientific | 2017 |
| Eliza Dewbury Designs Ltd | Professional & scientific | 2024 |
| Farriers Green (Management) Company Limited | Households | 1998 |
| Ferrers Park Management Company (Lechlade) Limited | Real estate | 2015 |
| Fifth Force Ltd | Professional & scientific | 2025 |
| Filkins Hall Residents Company Limited | Households | 1988 |
| Flux Dynamics Ltd | Professional & scientific | 2022 |
| Gale Gardner & Co Limited | Professional & scientific | 2000 |
| Gale Gardner Partners LLP | | 2025 |
| Getdecido Ltd | Professional & scientific | 2026 |
| Goose Catering Ltd | Hospitality | 2025 |
| Gordon T. Chambers (Holdings) Limited | Professional & scientific | 1979 |
| Gore-Grimes Productions Limited | Information & communication | 2026 |
| H R Management Solutions Limited | Professional & scientific | 2006 |
| H V Construction Services Limited | | 1993 |
| H2KDESIGNS Ltd | Manufacturing | 2005 |
| Harris Safety Solutions Ltd | Professional & scientific | 2014 |
| HB Online Solutions Ltd. | Wholesale & retail | 2025 |
| Heiten Limited | Administrative & support | 2025 |
| Hibbert Ralph Animation Limited | Arts & recreation | 1982 |
| Horse and Dolphin Limited | Construction | 1983 |
| Hubb Furniture Ltd | Wholesale & retail | 2025 |
| Hunt Risk Solutions Limited | Professional & scientific | 2018 |
| Inkpot Publishing Ltd | Information & communication | 2013 |
| International AG Solutions Ltd | Professional & scientific | 2021 |
| J.A. Cover Limited | Construction | 1986 |
| J.P.J. Communications Limited | Arts & recreation | 1981 |
| JBG Energy Ltd | Construction | 2026 |
| JCW Property Holdings LLP | | 2022 |
| Jghr Limited | Financial services | 2023 |
| Josser Enterprises Ltd | Hospitality | 2023 |
| Karen Todner Limited | Professional & scientific | 2018 |
| Kencot Hill Farm LLP | | 2022 |
| Kent Foundation Laboratories Limited | Arts & recreation | 1984 |
| Kingswick Limited | Wholesale & retail | 2014 |
| Kngm Sportswear Ltd | Manufacturing | 2025 |
| KS Leisure Services Ltd | Wholesale & retail | 2023 |
| Lanes Property Holdings Ltd | Financial services | 2025 |
| Langford Cricket Club Limited | Arts & recreation | 2021 |
| Langford Private Capital Ltd | Financial services | 2025 |